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期刊论文

Effect of 6-benzyladenine and casein hydrolysate on micropropagation of Amorpha fruticosa

郑国锠H.H. GAO W. LI* J. YANG Y. WANG G.Q. GUO and G.C. ZHENG

BIOLOGIA PLANTARUM 47 (1): 145-148, 2003/4,-0001,():

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摘要/描述

Using apical and axillary nodes as explants, a rapid and efficient method for propagation of Amorphafruticosa L. has been developed. When grown on Murashige and Skoog (MS) medium supplemented with 8mg dm-3 benzyladenine, 100% explants responded with 4.94 shoots per explant after 6-weeks culture, and explants taken from the in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same medium. The addition of casein hydrolysate (200mg dm"3) enhanced the number of shoots up to 8.77 per subculture, and coconut milk was found to promote the shoot elongation and make them grow more vigorously. 82.53% excised shoots were rooted on half-strength MS medium containing 2.0mg dm"3 indoleacetic acid after 3 weeks of incubation. After acclimatization, all of the rooted plantlets established in soil, exhibiting uniform morphological and growth characteristics.

版权说明:以下全部内容由郑国锠上传于   2005年02月25日 18时03分11秒,版权归本人所有。

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