Objectives: The effects of Chinese rhubarb on the experimental hyperlipoidemia were observed in rabbits. Methods: Thirty healthy male white rabbits were divided randomly into five groups: negative control group, rabbits were fed with common diet; experimental groups: the rabbits were fed with high lipid diet and administrated Introgastrically with different dosage of extracts of Chinese rhubarb (0.24, 0.7, 2.1 g/kg body weight); positive control group: the rabbits were fed with high lipid diet but no rhubarb extracts were administrated. Rabbits were fed for 10 weeks. Total cholesterol (TC), triglyceride (TG), high density poprotien cholesterol (HDL-C), and low density lipoprotein cholesterol (LDC-C) were determined in the beginning, the middle and the end of this experiment. Results” 1. TC concentration in experimental and positive groups were significantly higher than that of negative control group (P<0.01), which indicated the model was successfully made. Tc concentration in experimental groups were not significantly lower than that of the positive group except the high mubarb group in the middle period. (P<0.05). 2. TG concentration in negative control group was 1.11±0.20, 1.13±0.19; 1.12±0.23mmol/L at 0, 5 and 10 weeks, respectively. Ohterwise, the TG concentration at 10 weeks was 9.62±5.85m 4.37±1.54, 4.15±2.48, 4.27±1.60mmol/L in positive and experimental groups respectively. Compared with the positive group. TG concentration in experimental groups was significantly decreased in the same period especially at the 10 weeks after rhubarb administration (P<0.01). 3. At 10 weeks after rhubarb administration, LDL-C concentration was 1.05±0.20, 9.73±1.79m 7.16±0.88, 7.29±1.60, 6.35±0.69 in negative, positive and experimental group. Compared with the positive group, rhubarb could significantly reduced (P<0.01) the LDL-C concentration. 4. HDL-C concentration in the experimental groups was higher than that of positive group in the same period (P<0.01). Conclusion: This study indicated that Chinese rhubarb could decrease TG and LDL-C, and increase HDL-C in the atherosclerotic rabbits. The results above suggested that Chinese rhubarb has antiaherosclerotic effect. (Acknowledgements: This work is supported by Grant-in-Aids from the National Natural Science Fundation of China (No. 10572029).

根据犬瘟热病毒H 基因核苷酸序列，设计合成一对引物对贵州临床诊断为犬瘟热(CD) 病死犬的心、肝、脾、肾、粪便进行RT-PCR 检测。结果表明: 从心、脾、肾病料上清液中可扩增出760 bp 的特异性带，与预期扩增片段长度相同；粪便和肝脏上清液RT-PCR 结果为阴性，但粪便上清液接种Vero 细胞后连续传代3代，每代细胞培养液RT-PCR 结果均为阳性；在检测的22 条病死犬中，有19条病死犬检测到犬瘟热病毒H基因的特异性核酸片段，阳性率为8614 % (19/22) 。

将30只雄性健康新西兰白兔随机分为5组，每组6只。对照组给予基础饲料；模型组给予高脂饲料；三个大黄组给予高脂饲料同时分别用不同药量的大黄醇提液灌胃。于第10周末处死家兔，观察、测量和比较主动脉弓、胸主动脉、腹主动脉和髂动脉分支处血管脂质斑块的病理变化，计算斑块面积占胸主动脉的百分比；光镜下观察心脏冠状血管的病理改变，并计算冠状小动脉粥样硬化的发生率，研究大黄醇提液抑制家兔动脉粥样硬化形成的作用。结果显示：大黄醇提液能一定程度上抑制家兔主动脉及腹主动脉AS形成，减少AS斑块面积，降低动脉分支处AS的发生率，减轻心脏冠状动脉AS的病变。

目的：检验大黄醇提液抗家兔实验性高脂血症及脂肪肝的影响。方法：将30只雄性健康白兔随机分为5组(n=6)：对照组给予基础饲料；模型组给予高脂饲料；三个大黄组给予高脂饲料同时分剐灌胃不同药量的大黄醇提液。实验过程中进行一般性指标观测，检测不同阶段五组家兔血脂水平，检测脂肪肝病变程度。结果：大黄醇提液具有降低血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)，升高高密度脂蛋白胆固醇(FIDL-C)，降低肝细胞脂肪变性的作用。并且大黄醇提液的以上作用存在一定的量效关系。结论：大黄醇提液可降低动脉粥样硬化免模型的血脂水平、降低脂肪肝的发生发展。