赵德明
动物疾病分子病理学
个性化签名
- 姓名:赵德明
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学术头衔:
博士生导师
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学科领域:
畜牧科学、动物医学
- 研究兴趣:动物疾病分子病理学
赵德明,男,46岁,中国农业大学动物医学院副院长;国家动物海绵状脑病检测实验室主任;中国农业大学P3实验室主任;实验动物研究所副所长、教授,博士研究生导师,专业为动物病理学,侧重于动物疾病分子病理学。学术团体任职包括中国畜牧兽医学会理事;华北兽医病理学分会理事长;中国兽医病理学分会副理事长;北京市实验动物管理委员会专家委员;北京市政府顾问;第三届中国兽药典委员会委员;“Journal of Veterinary Science” 杂志编委会委员; 中国实验动物学报副主编; 全国兽医专业学位教育质量分析与跟踪调查委员会委员等。先后赴日本、美国、英国等国家和地区访问和合作研究。主持完成的项目包括农业部专项课题(农财200123)、国家教委课题(回国人员启动经费)、国家自然基金课题(39570554)、科技部疾病防治专项(2002BA518A07)、国家自然基金(30371062)。目前主持在研的项目包括:北京市科委(Z0004089040231)、教育部博士点基金(20020019006)、科技基础专项(2003DIB7J075)、北京市科委(H030730250190)。获奖情况:2003年度星火计划一等奖(主要完成人)、1997年度中国农业大学科技进步一等奖(第一完成人)、1999年教育部科技进步三等奖(第一完成人)、2000年北京市实验动物科技新星奖、第三届全国畜牧兽医科技工作者学术交流讨论会优秀论文奖(1996年)、北京市实验动物学会十五周年学术交流优秀论文奖(1998年)、北京市第五届青年优秀科技论文奖(1999年),获得专利“猪皮生物敷料的研制(专利号03108933.X, 2003)”。
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18
【期刊论文】Single nucleotide polymorphisms of the prion protein gene (PRNP) in Chinese pig breeds
赵德明, Liping Meng, Deming Zhao, , Hongxiang Liu, Jianmin Yang and Zhangyong Ning
Xenotransplantation 2005: 12: 324-326,-0001,():
-1年11月30日
Prion diseases (transmissible spongiform encephalopathies, TSE), as a group of fatal neurodegenerative diseases, have affected humans and a variety of other mammals. Although no natural TSE have been documented in pigs, appropriate precautions need to be taken to prevent the iatrogenic spread of prion disease through pig-to-human xenotransplantation. Polymorphisms within the open reading frame (ORF) of the single-copy gene of prion protein (PRNP) are associated with susceptibility to scrapie in sheep and variant Creutzfeldt-Jacob disease in humans. We screened polymorphisms in the PRNP gene of 64 China Experimental Minipigs and Beijing Large White pigs. Our findings suggest that the porcine PRNP gene is highly homogenous. The amino acid sequences of the mature prion protein of all samples tested were identical. Four single nucleotide polymorphisms (G11A, G615C, G684A, T726G) in the ORF of the porcine PRNP gene were found, and the G fi C nucleotide substitution resulted in a serine to asparaginate amino acid substitution at codon 4. We conclude that pigs raised under specific pathogen-free conditions, with the exclusion of rendered mammalian material for at least two generations, will have little risk of being infected with a TSE, and even less possibility of transmitting prion disease to humans through xenotransplantation.
bovine spongiform encephalopathy-pigs-prions-transmissible spongiform encephalopathies-xenotransplantation
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【期刊论文】云南矮马耳缘组织成纤维细胞系的建立及其生物学特性3
赵德明, 周向梅, , 马月辉, 关伟军
动物学报,50 (5): 863-868, 2004,-0001,():
-1年11月30日
A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the ex2 plant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell morphology and dynamic growth, analysis of karyotype and isoenzymes of lactate dehydrogenase and malate dehydrogenase were carried out. The expression of recombinant green fluorescence protein in the cells were also undertaken. The results showed that the population doubling time (PDT) of the cells was 24h; the frequency of cell chromosome number to be 2n=64 was 9219%; the banding patterns of the isozymes of the two enzymes had significant difference between the Yunnan pony ear marginal fibroblast cell line and the fibroblast cell lines of PEM 2/2, MSHEM 2/2 and BLCHE 2/2 derived from the Picdmont bovine ear, Mongolian ovine ear and Beijing local chicken embryo respectively. Tests for the contamination from bacteria, fungi or mycoplasma were negative; the transfection efficiency for the recombinant plasmid was 3213%. This newly established cell line make the Yunnan pony breed, a national important genetic resource preserved at cell level, as well as will provide an effective experimental material for genetic studies on the Yunnan pony
云南矮马, 耳缘组织, 成纤维细胞系, 生物学特性
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赵德明, Yali Cui a, b, Deming Zhao a, *, Hongxiang Liu c, Zhangyong Ning a, Jianmin Yang a, Xiuhui Qing a, Shumin Yu a, Changde Wu a
Maturitas 50(2005)337-343,-0001,():
-1年11月30日
Background: After menopause women are more susceptible to coronary heart disease due to increased risk of atherosclerosis. Tibolone (Livial) is an innovative synthetic steroid analogue for the treatment of postmenopausal climacteric symptoms including atherosclerosis, but the mechanisms of its effect are still unclear. The present study investigated the effect of tibolone and simvastatin on atherosclerosis and the expression of both estrogen receptor A (ERA) and LDL receptor (LDLR) mRNA in ovariectomized cholesterol-fed rabbits. Methods: Fifty New Zealand white rabbits were included for the study. Of them, 40 underwent bilateral ovariectomy and the other 10 were sham-operated. The sham-operated group only received atherogenic diet (group SC) and the ovariectomized rabbits were divided into 4 groups of 10 each, with group N received normal diet, group C received atherogenic diet, group T received atherogenic diet and tibolone (2.5mg/day) and group SI received atherogenic diet and simvastatin (20mg/day). After 12 weeks of the treatments, the animals were euthanized and the extent of thoracic aortic atherosclerosis was measured morphologically and the level of ERA and LDLR mRNA in heart and liver was determined by real-time RT-PCR. Results: The extent of atherosclerosis in the thoracic aorta was 0.75
Tibolone, Simvastatin, Atherosclerosis, Rabbit, Real-time RT-PCR, ERA, LDLR
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【期刊论文】Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line*
赵德明, X. M. Zhou, , Y. H. Ma, **, W. J. Guan and D. M. Zhao
,-0001,():
-1年11月30日
The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9h and 48h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.
Debao Pony,, Ear Marginal Tissue,, Fibroblast Cell Line,, Primary Explant Technique,, Collagenase Technique
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赵德明, JIAN-MIN YANG, l DE-MING ZHAO, l, * HONG-XIANG LIU, NING LI, YONG-XIN HAO, ZHANG-YONG NING & XIU-HUI QINl
Virus Genes 30:2, 193-196, 2005,-0001,():
-1年11月30日
The open reading frame of peacock and parakeet prion protein (PrP) genes was cloned and sequenced. The peacock and parakeet PrP genes consisted of 833 and 866 nucleotides encoding 266 and 277 amino acids, respectively (GenBank Accession numbers AY365065 and AY365066). Sequence analysis showed that the peacock and parakeet PrP genes had 93.67% homology to each other, 94.04% and 99.64% homology to the chicken PrP gene and 46.0% and 42.1% similarity to the mammalian PrP genes, respectively. The structural features of all known mammalian and avian PrPs, including N-terminal signal peptides, tandem repeats, conserved hydrophobic region, disulfide bridges and glycoinositol phospholipids anchor, were also found in peacock and parakeet PrPs. The parakeet and peacock PrPs, however, differed in the hexarepeat region, with the peacock having six and the parakeet having seven hexarepeats. The phylogenetic analysis showed that the PrP genes in 52 species were clustered into 2 distinct lineages, the avian and the mammalian. The peacock and parakeet PrP genes belonged to the same lineage but the peacock PrP was sub-classed with the pigeon PrP and the parakeet PrP was sub-classed with the duck and chicken PrPs. The present work added two more species data to the collection of the PrP genes and supported the previous findings that the PrP genes are highly conserved across species.
parakeet,, peacock,, phylogeny,, prion gene,, sequence analysis
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赵德明, L. P. Meng*, D. M. Zhao*, H. X. Liu†, J. M. Yang*, Z. Y. Ning*, C. D. Wu* and C. X. Han*
2005 International Society for Animal Genetics, Animal Genetics, 36, 258-286,-0001,():
-1年11月30日
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赵德明, Zhang-Yong Ning, De-Ming Zhao*, Jian-Min Yang, Ya-Li Cui, Li-Ping Meng, and Chang-De Wu, Hong-Xiang Liu
Animal Biotechmology, 16: 55-65, 2005,-0001,():
-1年11月30日
Determination of tissue-specific expression of cellular prion protein (prpc) is essential for understanding its poorly explained role in organisms. Herein we report on quantification of prp Mrna in golden hamsters,a popular experimental model for studying mechanisms of transmissible spongiform encephalopahies (TSE), by real-time RT-PCR.Total RNA was isolated from four different regions of the brain and six peripheral organs of eight golden hamsters Prp Mrna copy number were determined using absolute standard curve method with real-time quantitative PCR instrument.I was found that high Mrna levels were present in all four regions of the brain examined,ingunal lymph node showed high level of the expression similar to that in overall brain; spleen, heart, liver, and lung showed moder-ate levels of the expression; and kidney showed the lowest expression. Our result is consist-ent with the potential involveoment of different organs in prion diseases and offers essentialdata for further study of TSE mechanism in this animalmodel.
Prion, Golden hamster, Mrna expression, Real-time RT-PCR
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赵德明, 杨建民②, 赵德明③, 李宁, 宁章勇, 郝永新, 秦秀慧
高技术通讯,2004,13,29~32,-0001,():
-1年11月30日
根据已报道的哺乳动物朊病毒基因序列设计引物对,采用PCR方法扩增了大熊猫的朊病毒基因,将其克隆到T2Easy载体,序列测定及分析表明所克隆的大熊猫PRNP基因(GeneBank收录号为AF327449)片段为795bp,编码264个氨基酸的前体蛋白,推测其分子量约28.5ku。与已报道的牛(GeneBank收录号为AF455119)、绵羊(GeneBank 收录号为AF367623)的相应序列作比较分析,核苷酸序列同源性分别为99%和83%,其编码的氨基酸同源性均为100%。在所克隆的大熊猫PRNP基因中未发现与朊病毒敏感性连锁的氨基酸多态性位点。
大熊猫, 朊病毒, Prion 基因, 序列分析
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【期刊论文】疯牛病和羊痒病WESTERN BLOT检测方法的建立*
赵德明, 王辉暖, 赵德明**, 宁章勇, 杨建民, 吴常德, 郝俊峰, 白玉, 王传武, 孟丽平
,-0001,():
-1年11月30日
以朊蛋白单抗AH6和碱性磷酸酶标记的酶标马抗鼠二抗建立了疯牛病和羊痒病的Western Blot检测方法。对Western Blot各种反应条件进行摸索,并确定了最佳工作条件,结果表明:当匀浆缓冲液为RIPA时最佳反应条件为浓缩胶内电泳电压为恒压90V,分离胶电泳电压为恒压160V,转印的最佳电压和时间为恒压100V 1.5小时;封闭液为3%BSA时,封闭15分钟,封闭效果最好;AH6的最佳稀释浓度为1:4000,4℃下孵育过夜,马抗鼠二抗的最佳稀释浓度1:1000,室温下孵育30 分钟。采用已确立的反应条件对样品进行检测并与Prionics®-Check WESTERN进口试剂盒的检测结果比较,发现其敏感性为100%,特异性为99.4%,而进口试剂盒分别为100%,100%,与进口试剂盒无显著差异,这为在该基础上建立国产试剂盒提供了条件
疯牛病, 羊痒病, Western Blot
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赵德明, 宁章勇, 赵德明, 杨建民, 崔亚利, 孟丽平, 吴长德, 秦秀慧, 马李颖
,-0001,():
-1年11月30日
金黄地鼠是研究动物传染性海绵状脑病的理想模型动物之一,我们利用实时荧光定量RT-PCR技术,构建标准重组质粒制备标准曲线,对中枢神经系统的4个不同部位及外周6个组织提取总RNA,反转录后进行PrP基因表达的定量。结果发现,脑的四个检测部位都呈现高的表达量;在外周器官中,淋巴结的表达量和全脑相当,脾脏、心脏、肝脏和肺脏呈现中等程度的表达,肾脏的表达量最低。本研究的结果对于探讨朊蛋白的基本功能和不同组织在传染性海绵状病理发生中的作用,提供了基础数据。
金黄地鼠, 朊蛋白, 基因表达
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