童建
主要研究方向为分子毒理学和健康危险度评价
个性化签名
- 姓名:童建
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学术头衔:
博士生导师
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学科领域:
毒理学
- 研究兴趣:主要研究方向为分子毒理学和健康危险度评价
童建,男,教授、博士。1953年5月出生,籍贯江西清江。1988年毕业于上海医科大学,博士学位;1990-1992年赴美国纽约州立大学,从事博士后访问研究;1993年回国任放射医学系副主任,副教授,1997年任教授、博导;2000年任苏州大学核医学院副院长;2002年担任苏州大学核医学院院长;放射医学国家级重点学科学科带头人,江苏省“333工程”学术带头人,先后主持两项国家自然科学基金、国际原子能机构援助项目($18.8万)、国防预研重点项目(50万元)等。目前承担国际原子能机构、国家自然科学基金和部省级科研课题多项,主要研究方向为分子毒理学和健康危险度评价。研究成果已获省部级科技进步二等奖1项,三等奖3项。在国内外专业期刊发表学术论文70余篇,主编和参编专著5部。主要社会兼职包括国家原子能机构专家组成员、中国毒理学会和中国核学会理事等。担任《中华放射医学与防护杂志》等8种国内外期刊的常务编委或编委。
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主页访问
2049
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关注数
0
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成果阅读
285
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成果数
7
【期刊论文】Toxicokinetics of Fenvalerate Mixed with Phoxim in Mice
童建, Jian TONG, Zeng-Li ZHANG
JOccup Health 44(2002)103-104,-0001,():
-1年11月30日
Phoxim and fenvalerate are pesticides that are most widely used as commercial formulae in China. Fenvalerate belongs to the pyrethroid compounds [Benzeneacetic acid, 4-chloro-α-(1-methylethyl)-, cyano (3-phenoxyphenyl) methyl ester. CAS registry number: 51630-58-1], while phoxim is an organic phosphate (3,5- dioxa-6-aza-4-phosphaoct-6-ene-8-nitrile, 4-ethoxy-7- phenyl-, 4-sulfide. CAS registry number: 14816-18-3). The metabolism of fenvalerate has been studied in vivo1), but the metabolism and distribution of its mixture with phoxim have not been reported in literature. A recent epidemiological investigation showed that the incidence of poisoning caused by the two mixed pesticides was three times higher than that by either one alone under similar exposure conditions2). The purpose of this study was to determine the toxicokinetics of these pesticides in order to provide information for furthering the toxicity mechanism.
Toxicokinetics,, Fenvalerate,, Phoxim,, Mice
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童建, 洪承皎, 王静, 傅春玲
辐射研究与辐射工艺学报,2001,19(3):236~238,-0001,():
-1年11月30日
将大鼠暴露于10 、20 和30WLM(working level months)的氡浓度后,采用H-TdR掺入法和碱性单细胞凝胶电泳技术(SCGE),研究吸入氡及其子体后大鼠外周血淋巴细胞的DNA 损伤效应。结果表明,各暴露剂量组大鼠外周血淋巴细胞转化均显著低于对照组;淋巴细胞在碱性单细胞凝胶电泳条件下DNA 的迁移距离均显著高于对照组,且呈剂量- 效应关系。大鼠吸入氡及其子体可引起淋巴细胞DNA 合成抑制及淋巴细胞转化能力下降。
氡及其子体, 淋巴细胞, DNA损伤
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【期刊论文】Effect of 7-hydroxystaurosporine on glioblastoma cell invasion and migration1
童建, Qing-hui MENG, Li-xin ZHOU, Jia-lin LUO, Jian-ping CAO, Jian TONG, Sai-jun FAN,
Acta Pharmacologica Sinica 26 4(2005)492-499,-0001,():
-1年11月30日
Aim: To investigate the effect of 7-hydroxystaurosporine (UCN-01), a selective protein kinase C (PKC) inhibitor, on cell growth, migration, and invasion in invasive human glioblastoma U-87MG cells. Methods: PKC activity was determined based on the PKC-catalyzed transfer of the 32P-phosphate group from [g-32P]ATP into a PKC-specific peptide substrate. Cell viability was measured by MTT assay. Cell invasion and migration were evaluated by a Boyden chamber assay and scratch wound assay, respectively. Protein expression was analyzed using Western blot assay. The formation of 3-dimensional cellular aggregates was examined by a cell-cell aggregation assay. Results: UCN-01 treatment resulted in concentration-and time-dependent inhibition of U-87MG cell growth at higher doses (> 100 nmol/L), and reduced cell invasion and migration capability at less cytotoxic doses (<100 nmol/L). UCN-01 significantly repressed PKC activity. Consistent with this result, UCN-01 blocked cell invasion stimulated by phorbel 12-myristate- 13-acetate (PMA) and ethanol (EtOH), 2 PKC activators. Enforced expression of the tumor suppressor genes BRCA1 and PTEN increased the anti-invasion potential of UCN-01. Exposure to UCN-01 caused a dose-dependent increase in cell adhesion molecule E-cadherin. The effect of UCN-01 on the formation of cell-cell aggregation was significantly reduced by the addition of an anti-E-cadherin antibody. Conclusion: UCN-01 inhibits the invasion and migration of human glioma cells. Accordingly, UCN-01 can have potential clinical applications for the treatment of human glioma metastasis.
7-hydroxystaurosporine, protein kinase C, cell movement, cadherins, BRCA1 protein
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童建, Yu-zhen Du a, Sai-jun Fan a, b, Qing-hui Meng b, Guo-qing Wang a, Jian Tong a, *
Biochemical and Biophysical Research Communications 336(2005)1069-1073,-0001,():
-1年11月30日
In this paper, the circadian pattern of Clock and genes mediated by the Clock was investigated in peripheral lymphocytes of rats. Circadian rhythms of Clock are found under the regimes of constant darkness (DD) and 12-h light–12-h dark (LD12:12 h), with the peak phase at CT7 and ZT21, respectively. Ten differential cDNA fragments were identified to be mediated by the Clock, including three known genes (catalase, myelin proteolipid protein, and histone acetylase), four known expressed sequence tags (ESTs), and three novel ESTs. Experiment of the RNA interference revealed that these ESTs were down-regulated by the Clock gene and three of them were identified as clock-controlled genes. Understanding of clock-mediated genes may lead to a new direction in drug design for control of circadian rhythms.
Clock gene, Clock-controlled genes, Lymphocytes
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【期刊论文】大鼠松果体Clock基因和芳烷脘N-乙酞基转移酶基因的昼夜节律性表达及光照影响
童建, 王国卿, , 杜玉珍, 童建.*
生理学报,2005,57(1):97~102,-0001,():
-1年11月30日
摘要:探讨12h光照、12h黑暗交替(12 h-light:12 h-dark cycle,LD)及持续黑暗(constant darkness,DD)光制下松果体Clock基因和芳烷脘N-乙酰基转移酶基因(arylalkylamineⅣ-acetyltransferase gene,NAT)是否存在昼夜节律性表达及其光反应变化。Sprague-Dawley大鼠在LD和DD光制下分别被饲养4周(n=36)和8周n=36)后,在一昼夜内每隔4h采集一组松果体组织(n=6),提取总RNA,用竞争性定量RT-PCR测定不同昼夜时点样品中Clock及NAT基因的mRNA相对表达量,通过余弦法和Clock Lab软件获取节律参数,并经振幅检验是否存在昼夜节律。结果如下:(1)在DD或LD光制下,松果体Clock和NAT基因mRNA的表达均呈现夜高昼低的节律性振荡(P<0.05)。(2)与DD光制下比较,LD光制下松果体Clock和NAT基因的表达振幅及峰值相的mRNA水平均降低(P<0.05)。(3)在DD或LD光制下,Clock和NAT基因之间显示相似的节律性表达(P>0.05)。结果表明,Clock和NAT基因在松果体中存在同步的内源性昼夜节律表达,光照作用可使其表达下调。
Clock基因, NAT基因, 昼夜节律, 光照, 松果体
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童建, 王洁
中华放射医学与防护杂志,2005,25(2):205~207,-0001,():
-1年11月30日
蛋白质组(proteome) 一词由Wilkin's 等于1996 年首先提出,用来描述一个细胞、组织或有机体所表达的所有蛋白质[1-3 ] 。蛋白质组学(proteomics) 则是研究生物系统内特定时间或特定条件下蛋白质表达规律的科学[1 ,3 ] 。基因组是生命体遗传信息的载体,而蛋白质组是生命活动的执行体。不同细胞或生物体的基因组结构有许多相似的基本特征,并且相对稳定。而蛋白质组却是动态变化的,在细胞周期和细胞分化的不同阶段,以及各种不同的营养或环境条件下,蛋白质组都会表现出显著的差异。更重要的是,在基因和蛋白质之间并不存在完全对应的关系,即一个开放阅读框架并不一定预示存在一个相应的功能性蛋白;mRNA表达水平也并不等同于蛋白质表达水平。此外,体内蛋白质合成存在磷酸化/去磷酸化、甲基化/去甲基化等动态修饰和加工过程;蛋白质与蛋白质之间存在着相互作用;许多蛋白质需要和其他分子结合后才能显示出生物学功能。对于这些复杂的生命活动,必须通过蛋白质组学的研究才可能予以阐明。
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【期刊论文】Toxicokinetics of Fenvalerate Mixed with Phoxim in Mice
童建, Jian TONG, Zeng-Li ZHANG
J Occup Health 44(2002)103-104,-0001,():
-1年11月30日
Phoxim and fenvalerate are pesticides that are most widely used as commercial formulae in China. Fenvalerate belongs to the pyrethroid compounds [Benzeneacetic acid, 4-chloro-α-(1-methylethyl)-, cyano (3-phenoxyphenyl) methyl ester. CAS registry number: 51630-58-1], while phoxim is an organic phosphate (3,5- dioxa-6-aza-4-phosphaoct-6-ene-8-nitrile, 4-ethoxy-7- phenyl-, 4-sulfide. CAS registry number: 14816-18-3). The metabolism of fenvalerate has been studied in vivo1), but the metabolism and distribution of its mixture with phoxim have not been reported in literature. A recent epidemiological investigation showed that the incidence of poisoning caused by the two mixed pesticides was three times higher than that by either one alone under similar exposure conditions2). The purpose of this study was to determine the toxicokinetics of these pesticides in order to provide information for furthering the toxicity mechanism.
Toxicokinetics,, Fenvalerate,, Phoxim,, Mice
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