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郑从义

长期从事病毒感染细胞生物学和低温生物学的研究

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姓名：郑从义
目前身份：
担任导师情况：
学位：
学术头衔：

博士生导师
职称：-
学科领域：

微生物学
研究兴趣：长期从事病毒感染细胞生物学和低温生物学的研究

个人简介

　郑从义，教授、博士生导师，现任武汉大学中国典型培养物保藏中心主任。工作经历：1.1977年毕业于武汉大学生物系留校任教；2.1988年赴美国ATCC(American Type Culture Collection)作访问学者；3.1992年10月应WFCC邀请，作为专家在国际培养物保藏技术培训班上指导动物细胞保藏实验；4.1993年，赴日本参加国际低温生物学学术年会。
　　长期从事病毒感染细胞生物学和低温生物学的研究。主持中国专利细胞培养物保藏研究工作，收集、保藏来自12个国家和地区的专利细胞培养物300余株，非专利动物细胞系300余株。并长期从事微生物学、低温生物学、病毒感染细胞生物学的教学和科研。主讲微生物学、医学微生物学、低温生物学、免疫学基础、高级微生物学专题等本科生、研究生课程。近年来先后承担国家"973"、"863"项目，国家科技部、教育部重点资助项目，企业合作项目20余项。2003年建立了感染、分离SARS病毒的细胞模型，并从湖北SARS患者病料中分离到SARS病毒（WHU株），被评为全国抗击非典型肺炎优秀科技工作者。在国内外刊物上发表科学论文60余篇，出版论著2部，参编教材2部，获得国家发明专利1项。

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2009-03-17

【期刊论文】Short communication The difference of energy metabolism between persistent and cytocidal virus infections

郑从义， Liu Yuwena， Zhang Henga， Wang Cunxin a， Li Jiea， Li Huitin a， Li Zhaoyang b， Wu Jibin b， Zheng Congyi b， ∗

Thermochimica Acta 407(2003)113-116，-0001，（）：

-1年11月30日

摘要

In this study, microcalorimetric measurements were carried out on PK-15 cell line persistently infected by cholera swine fever virus (CSFV) and BHK-21 cell line infected by foot and mouth disease virus (FMDV). The aim of this investigation was to investigate the difference of energy metabolism between the different kinds of virus infections-persistent infection and cytocidal infection. The thermogenesis curves determined by using a LKB 2277 Bioactivity Monitor were significantly different. From these thermogenic curves, the maximum heat production rate Pm and total heat output Q are obtained. The results show that energy metabolism is different between persistent infection and cytocidal infection.

Virus， Microcalorimetry， Metabolism， Persistent infection， Cytocidal infection

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2009-03-17

【期刊论文】G1-phase specific apoptosis in liver carcinoma cell line induced by copper-1,10-phenanthroline

郑从义， Hui Zhoua， Congyi Zheng a， Guolin Zoua， ∗， Deding Tao b， Jianping Gongb

The International Journal of Biochemistry & Cell Biology 34(2002)678-684，-0001，（）：

-1年11月30日

摘要

Reactive oxygen species play an important role in the mediation of cell killing. But the mechanistic links between reactive oxygen species (ROS) and cell death remains unclear. There was a speculation that ROS, especially hydroxyl radicals can induce necrosis but not apoptosis in cells treated with copper-1,10-phenanthroline, IICu(OP)2. In this paper, liver carcinoma cell line (Bel-7402) was treated with IICu(OP)2 and its effect was examined by several means. Cells were found to undergo changes characteristic of apoptosis. Hoechst staining showed apoptotic body appeared in the cells induced by IICu(OP)2. When DNA extracted from the cells treated with IICu(OP)2 was analyzed by agarose gel electrophoresis it generated 'ladder' pattern of discontinuousDNAfragments. Sub-G1 peakwas detected in treated cells. Furthermore, two different flowcytometric methods were used, each allowing us to relate the apoptotic cells to the position the cell-cycle position. Apoptosis induced by IICu(OP)2 was limited to G1-phase cells. Using cyclin analysis, the expression of cyclin E in G1 was blocked. Thus, it was concluded that IICu(OP)2 can induceG1-phase specific apoptosis in Bel-7402.

Copper-1,， 10-phenanthroline， Apoptosis， Reactive oxygen species， Flow cytometry

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2009-03-17

【期刊论文】EXTREMELY LOW FREQUENCY (ELF) PULSED-GRADIENT MAGNETIC FIELDS INHIBIT MALIGNANT TUMOUR GROWTH AT DIFFERENT BIOLOGICAL LEVELS

郑从义， XINCHEN ZHANG， HUSHENG ZHANG*， CONGYI ZHENG， CHAOYANG LI， XINSONG ZHANG and WEI XIONG

Cell Biology International 2002, Vol. 26, No.7, 599-603，-0001，（）：

-1年11月30日

摘要

Extremely low frequency (ELF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100T • M1, pulse width of 20-200 ms and frequency of 0.16-1.34Hz treatment of mice can inhibit murine malignant tumour growth, as seen from analyses at different hierarchical levels, from organism, organ, to tissue, and down to cell and macromolecules. Such magnetic fields induce apoptosis of cancer cells, and arrest neoangiogenesis, preventing a supply developing to the tumour. The growth of sarcomas might be amenable to such new method of treatment.

extremely low frequency (， ELF)， pulsed-gradient magnetic field， malignant tumour， inhibitory， biological levels.，

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2009-03-17

【期刊论文】Microcalorimetric study of the metabolism of U-937 cells undergoing apoptosis induced by the combined treatment of hyperthermia and chemotherapy

郑从义， Liu Yuwena， Wang Cunxina， Zheng Congyib， *， Wu Haixiangb， Wang Zhiyonga， Qu Songshenga

Journal of Thermal Biology 27(2002)129-135，-0001，（）：

-1年11月30日

摘要

Hyperthermia is a useful adjunct in cancer therapy, as it can increase the effectiveness and decrease the toxicity of currently available cancer treatments such as chemotherapy and radiation. In this study we determined the power-time curves of U-937 cell line treated by the combination of hyperthermia and Carmofur by using an LKB 2277 Bioactivity Monitor. The maximal thermal power and the heat production were used to evaluate the antitumor effect. Our results show that the combined treatment of hyperthermia and Carmofur had a synergistic antitumor effect, which is consistent with the apoptosis ratio obtained by TUNEL assay. The results also indicate that the metabolic activity of apoptotic cells is lower than that of normal cells. Thus microcalorimetry is a powerful tool in fields of hyperthermia.

Microcalorimetry， Hyperthermia， Metabolism， Apoptosis， TUNEL

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2009-03-17

【期刊论文】Copper Complex of Hydroxyl-Substituted Triazamacrocyclic Ligand and Its Antitumor Activity

郑从义， Feng Liang， a Chengtai Wu， a， * Huakuan Lin， b Tao Li， c Dongzhao Gao， b Zhaoyang Li， d Jun Wei， d Congyi Zhengd and Mengxiang Sund

Bioorganic & Medicinal Chemistry Letters 13(2003)2469-2472，-0001，（）：

-1年11月30日

摘要

The protonation constants and the stability constants for the formation of copper (II) complex of the ligand [1,4,7] Triazecan-9-ol (L) were presented. Antitumor activity of CuL complex was reported. Preliminary pharmacological tests showed that it had antitumor activity against HXO-RB44 and BEL-7402 cell lines in vitro. Nuclei of [CuL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to BEL-7402 cell lines cycle and further studies showed that it could cleave supercoiled DNA (pBR 322) to nicked and linear DNA.

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2009-03-17

【期刊论文】Synthesis and preliminary evaluation of gadolinium complexes containing sulfonamide groups as potential MRI contrast agents

郑从义， Guo-Ping Yan， Ph.D， Professor* Cong-Yi Zheng， Professory Wei Cao， M.D.z Wei Li， M.S.* Li-Yun Li， Professor

Radiography (2003) 9, 35-41，-0001，（）：

-1年11月30日

摘要

Four DTPA and DOTA derivative ligands containing sulfonamide groups and their gadolinium complexes were prepared and characterized. Relaxivity studies showed that these gadolinium complexes possessed higher relaxation effectiveness than those of the corresponding ionic gadolinium complex Gd-DTPA and Gd-DOTA, respectively. In vitro cytotoxicity assay demonstrated that these gadolinium complexes have low cytotoxicity to the human liver cells (L-02). Magnetic resonance imaging (MRI) and experimental data of biodistribution in mice indicated that these gadolinium complexes containing sulfonamide groups could be used as the potential MRI contrast agents for Hepatoma (H22) and Ehrlich ascites carcinoma (EAC) in mice and specific organs such as the liver and could be mostly excreted by the kidneys.

magnetic resonance imaging (， MRI)，， gadolinium complexes， sulfanilamide， sulfadiazine， relaxivity， target-specific.，

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2009-03-17

【期刊论文】构建猪瘟病毒致细胞病变的PK-15细胞模型

郑从义，吴海祥，张楚瑜*，王家富，潘兹书，李蕾，曹晟，伊光辉

科学通报，2004，48（8）：822～826，-0001，（）：

-1年11月30日

摘要

猪瘟病毒在离体细胞培养条件下不引起宿主细胞病变，病毒与宿主细胞之间相互作用的研究一直没有合适的模型以猪瘟病毒中国标准强毒石门株为材料，通过基因工程的手段，在构建全基因组感染性克隆的基础上，对全基因组进行部分缺失，获得了7.5kh的亚基因组.以携带野生型猪瘟病毒的PK-15细胞为宿主，用亚基因组进行转染，获得到了能够诱导PK-15细胞产生CPE的亚基因组病毒，检测显示产生CPE的细胞培养物中野生型基因组和亚基因组共同存在猪瘟病毒致细胞病变模型的构建，为进一步研究其致病的分子机制开辟了新的方向。

猪瘟病毒，基因组感染性克隆，亚基因组，干扰缺损颗粒，致细胞病变效应

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2009-03-17

【期刊论文】Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors

郑从义， Jieqing Zhu， a， Gengfu Xiao， b， Yanhui Xu， c， Fang Yuan， d Congyi Zheng， b Yueyong Liu， a Huimin Yan， b David K. Cole， d John I. Bell， d Zihe Rao， c Po Tien， * and George F. Gaoa， d， *

Biochemical and Biophysical Research Communications 319(2004)283-288，-0001，（）：

-1年11月30日

摘要

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GSTfusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.

SARS-CoV， Coronavirus， Spike(， S)， protein， Fusion core， Heptad repeat (， HR1 and HR2)，， Inhibition

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2009-03-17

【期刊论文】丙肝病毒全基因组cDNA克隆侵染细胞培养体系的建立

郑从义，姚相杰①，郭佳①，郑从义①*，方呈祥①，屈三甫①，陈震球②，李中红②，李信墙②*，李卫云②

科学通报，2004，49（10）：965～970，-0001，（）：

-1年11月30日

摘要

以含有丙肝病毒全基因组 cDNA的重组质粒（pHCV）为材料, 通过基因转染、重组痘病毒辅助使之在HeLa细胞中表达HCV病毒体，建立了一种新的HCV体外细胞培养系统。采用RT-PCR，荧光定量RT-PCR，链特异性RT-PCR，免疫印迹，免疫电子显微镜和电子显微镜负染技术跟踪检测HCV的增殖效率，结果显示，该培养体系中有HCV正链RNA的合成和HCV结构蛋白、非结构蛋白的表达，并组装成直径约47nm的HCV病毒体，病毒体可被偶联有胶体金的抗HCV结构蛋白E2的单抗标记；该体系产生的HCV病毒体滴度达107基因拷贝/mL，远远高于病人血清中的HCV基因拷贝数，也高于迄今报道的任何一种HCV细胞培养体系的病毒滴度，检测结果证明，转染细胞裂解上清中的HCV病毒体可以感染肝癌细胞系Huh7，并在感染细胞中增殖和合成负链RNA中间体，病毒滴度达106基因拷贝/mL。

丙型肝炎病毒(， HCV)，，全基因组，侵染性cDNA，克隆，重组痘病毒，细胞培养体系

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2009-03-17

【期刊论文】Nickle(II) and cobalt(II) complexes of hydroxyl-substituted triazamacrocyclic ligand as potential antitumor agents

郑从义， Feng Liang， a Ping Wang， a Xiang Zhou， a Tao Li， b Zhaoyang Li， c Huakuan Lin， d， Dongzhao Gao， d Congyi Zhengc and Chengtai Wua， *

Bioorganic & Medicinal Chemistry Letters 14(2004)1901-1904，-0001，（）：

-1年11月30日

摘要

The stability constants for the formation of nickle(II) and cobalt(II) complexes of the ligand [1,4,7]triazecan-9-ol (L) were presented. Antitumor activity of two complexes was reported. Nuclei of [NiL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to cell cycle, and optimal induction of apoptosis was found by Flow-Cytometric analysis. But CoL complex did not exhibit introduction effects to BEL-7402 cells apoptosis; and could not perturb cell cycle. NiL and CuL complexes could cleave supercoiled DNA (pBR 322 DNA) to nicked and linear DNA, and DNA of cells treated with NiL or CuL complex was obviously damaged; while CoL complex only could cleave supercoiled DNA (pBR 322 DNA) to nicked DNA, and DNA of cells treated with CoL complex had no significant difference with control.

Macrocyclic polyamines， Metal complexes， Antitumor activity， DNA cleavage.，

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2009-03-17

【期刊论文】Synthesis and preliminary evaluation of gadolinium complexes containing sulfonamide groups as potential MRI contrast agents

郑从义， Guo-Ping Yan， Ph.D， Professor* Cong-Yi Zheng， Professory Wei Cao， M.D.z Wei Li， M.S.* Li-Yun Li， Professor

Radiography (2003) 9, 35-41，-0001，（）：

-1年11月30日

摘要

magnetic resonance imaging (， MRI)，， gadolinium complexes， sulfanilamide， sulfadiazine， relaxivity， target-specific.，

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2009-03-17

【期刊论文】Short communication The difference of energy metabolism between persistent and cytocidal virus infections

郑从义， Liu Yuwena， Zhang Henga， Wang Cunxin a， Li Jiea， Li Huitin a， Li Zhaoyang b， Wu Jibin b， Zheng Congyi b， ∗

Thermochimica Acta 407(2003)113-116，-0001，（）：

-1年11月30日

摘要

Virus， Microcalorimetry， Metabolism， Persistent infection， Cytocidal infection

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2009-03-17

【期刊论文】丙肝病毒全基因组cDNA克隆侵染细胞培养体系的建立

郑从义，姚相杰①，郭佳①，郑从义①*，方呈祥①，屈三甫①，陈震球②，李中红②，李信墙②*，李卫云②

科学通报，2004，49（10）：965～970，-0001，（）：

-1年11月30日

摘要

丙型肝炎病毒(， HCV)，，全基因组，侵染性cDNA，克隆，重组痘病毒，细胞培养体系

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2009-03-17

【期刊论文】Copper Complex of Hydroxyl-Substituted Triazamacrocyclic Ligand and Its Antitumor Activity

郑从义， Feng Liang， a Chengtai Wu， a， * Huakuan Lin， b Tao Li， c Dongzhao Gao， b Zhaoyang Li， d Jun Wei， d Congyi Zhengd and Mengxiang Sund

Bioorganic & Medicinal Chemistry Letters 13(2003)2469-2472，-0001，（）：

-1年11月30日

摘要

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2009-03-17

【期刊论文】G1-phase specific apoptosis in liver carcinoma cell line induced by copper-1,10-phenanthroline

郑从义， Hui Zhoua， Congyi Zheng a， Guolin Zoua， ∗， Deding Tao b， Jianping Gongb

The International Journal of Biochemistry & Cell Biology 34(2002)678-684，-0001，（）：

-1年11月30日

摘要

Copper-1,， 10-phenanthroline， Apoptosis， Reactive oxygen species， Flow cytometry

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2009-03-17

【期刊论文】Nickle(II) and cobalt(II) complexes of hydroxyl-substituted triazamacrocyclic ligand as potential antitumor agents

郑从义， Feng Liang， a Ping Wang， a Xiang Zhou， a Tao Li， b Zhaoyang Li， c Huakuan Lin， d， Dongzhao Gao， d Congyi Zhengc and Chengtai Wua， *

Bioorganic & Medicinal Chemistry Letters 14(2004)1901-1904，-0001，（）：

-1年11月30日

摘要

Macrocyclic polyamines， Metal complexes， Antitumor activity， DNA cleavage.，

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2009-03-17

【期刊论文】EXTREMELY LOW FREQUENCY (ELF) PULSED-GRADIENT MAGNETIC FIELDS INHIBIT MALIGNANT TUMOUR GROWTH AT DIFFERENT BIOLOGICAL LEVELS

郑从义， XINCHEN ZHANG， HUSHENG ZHANG*， CONGYI ZHENG， CHAOYANG LI， XINSONG ZHANG and WEI XIONG

Cell Biology International 2002, Vol. 26, No.7, 599-603，-0001，（）：

-1年11月30日

摘要

extremely low frequency (， ELF)， pulsed-gradient magnetic field， malignant tumour， inhibitory， biological levels.，

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2009-03-17

【期刊论文】Microcalorimetric study of the metabolism of U-937 cells undergoing apoptosis induced by the combined treatment of hyperthermia and chemotherapy

郑从义， Liu Yuwena， Wang Cunxina， Zheng Congyib， *， Wu Haixiangb， Wang Zhiyonga， Qu Songshenga

Journal of Thermal Biology 27(2002)129-135，-0001，（）：

-1年11月30日

摘要

Microcalorimetry， Hyperthermia， Metabolism， Apoptosis， TUNEL

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2009-03-17

【期刊论文】构建猪瘟病毒致细胞病变的PK-15细胞模型

郑从义，吴海祥，张楚瑜*，王家富，潘兹书，李蕾，曹晟，伊光辉

科学通报，2004，48（8）：822～826，-0001，（）：

-1年11月30日

摘要

猪瘟病毒，基因组感染性克隆，亚基因组，干扰缺损颗粒，致细胞病变效应

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2009-03-17

Biochemical and Biophysical Research Communications 319(2004)283-288，-0001，（）：

-1年11月30日

摘要

SARS-CoV， Coronavirus， Spike(， S)， protein， Fusion core， Heptad repeat (， HR1 and HR2)，， Inhibition

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