毕阳
从事果蔬采后生物学与技术以及食品芳香物质方面的科研工作
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- 姓名:毕阳
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学术头衔:
博士生导师
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食品科学技术
- 研究兴趣:从事果蔬采后生物学与技术以及食品芳香物质方面的科研工作
毕阳,男,汉族,1962年生,河北省井陉县人。甘肃农业大学食品科学与工程学院院长、教授、博士研究生导师。《甘肃农业大学学报》副主编、编委,《中国农业科学》编委,河西走廊“星火”产业带专家顾问组副组长,教育部高等学校高职高专食品类专业教学指导委员会副主任委员,甘肃省政协委员。
1982年毕业于甘肃农业大学园艺系,获学士学位;1989年毕业于新疆农业大学农产品贮藏加工专业,获硕士学位;2006年毕业于兰州大学生命科学院植物学专业,获博士学位。分别于2002年和2003年以高级访问学者的身份赴法国国家农业研究院(INRA)农产品质量与安全研究所、植物细菌研究所和中国科学院植物研究所进行采后诱导抗病性合作研究。2007年在瑞士西北应用科技大学进行培训和有机食品方面的调研。曾赴澳大利亚、泰国讲学并参加国际学术研讨会。
甘肃省“333”科技人才工程第一、二层次人选。曾获甘肃省中青年教师成才奖。为食品科学与工程、食品质量与安全专业的本科生讲授《采后生物学与技术》和《农产品贮运学》,为农产品贮藏与加工、食品科学和果树学专业的硕士研究生讲授《采后生物学与技术》、《采后病理学》和《农产品贮藏保鲜技术》。主持完成的《果蔬贮运学实验教学的改革与实践》获甘肃省教学成果二等奖。
担任植物病理专业博士研究生导师,农产品贮藏与加工、食品科学和果树学专业的硕士研究生导师,主要指导研究生进行果蔬采后病理、采后生理、贮运技术以及食品芳香物质等方面的研究工作。已培养硕士毕业生15名。目前指导在读硕士研究生15人。主要从事果蔬采后生物学与技术以及食品芳香物质方面的科研工作。尤其专长于采后抗病性的诱导和安全防腐。已完成《哈密瓜防腐技术的研究与推广》、《苹果梨黑皮病发病机理及控制》等省部级科研项目8项,获省部级科技进步三等奖3项,地厅级科技进步二等奖1项,申请专利2项。
现主持国家自然科学基金项目1项(《采后可溶性硅处理对厚皮甜瓜果实抗病性的诱导机理研究》、国家科技支撑计划子项目3项(《梨果实采后主要生理病害的控制技术研究》、《李果实冷害的综合控制技术优化与示范》、《枸杞、葡萄、啤酒花产业提升关键技术研究与示范》),国家“星火”科技项目1项(《河西走廊啤酒麦芽加工企业关键生产工艺技术的提升与完善》),甘肃省科技支撑和“星火”科技项目各1项。主编专著《果蔬采后病害与控制》(中国农业出版社)、《蔬菜制品加工工艺与配方》(化工出版社),副主编统编教材《果蔬贮藏与加工》(中国农业科技出版社)和《果蔬采后生理与生物技术》(中国农业出版社)。
在《Postharvest Biology and Technology》、《Plant Disease》、《Stewart Postharvest Review》、《Acta Horticulturae》、《Journal of Phytopathology》、《Agricultural Sciences in China》、《食品科学》、《食品工业科技》、《农业工程学报》、《植物病理学报》、《植物保护学报》、《园艺学报》、等国内外学术刊物及国际会议论文集上发表论文80余篇。代表性论文有:①Bi Yang, Ge Yonghong, Li Yongcai and Wang Junjie, Mao Xiaoying and Li Xuewen. Postharvest acibenzolar-S-methyl treatment suppress decay and induces resistance in Hami melon. Acta Horticulturae. 2006, 1: 393-399;②Bi Yang, Tian Shiping, Guo Yurong, Ge Yonghong, and Qin Guozheng. Sodium silicate reduces postharvest decay on Hami melons: induced resistance and fungistatic effects. Plant Disease. 2006, 90(3): 279-283;③Bi Yang, Tian Shiping, Zhao Jie, Ge Yonghong. Harpin induces local and systemic resistance against Trichothecium roseum in harvested Hami melons. Postharvst Biology and Technology. 2005, 38: 183-187等。
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毕阳, Yurong Guo, Lei Liu, Jian Zhao* & Yang Bi
International Journal of Food Science and Technology 2007, 1012 42, 1012-1018,-0001,():
-1年11月30日
Silicon oxide and sodium silicate were investigated as potential agents for the control of postharvest pink rot in Chinese cantaloupe (cultivar Yujingxing) caused by Trichothecium roseum. In vitro tests showed that sodium silicate, when added to potato dextrose agar, was effective in suppressing the radial growth of the pathogen on the medium, whereas silicon oxide was ineffective. The effectiveness of sodium silicate increased with concentration, and the growth of the fungus was completely inhibited at 100 mm. When melons were dipped in the solutions, both silicon oxide and sodium silicate significantly (P < 0.01) reduced the severity of pink rot of the cantaloupe with lesion diameters reduced by up to fivefold when compared with the controls. Scanning electron microscopy-energy dispersive X-ray analysis showed that silicon (Si)-treated melons had a smoother surface feature and higher Si levels in the epidermis, especially at the stomata and along the junction between the exocarp and mesocarp. Enhanced peroxidase and phenylalanine ammonia lyase activities were observed in sodium-silicate-treated melons but not in those treated with silicon oxide. The results indicate that different mechanisms might be involved in sodium silicate and silicon-oxide-initiated reduction of postharvest pink rot in Chinese cantaloupe.
Cantaloupe, peroxidase, phenylalanine ammonia lyase, pink rot, scanning electron microscopy-energy dispersive X-ray, silicon oxide, sodium silicate, Trichothecium roseum.,
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【期刊论文】Effect of temperature on chilling injury, decay and quality of Hami melon during storage
毕阳, Bi Yang a, b, Tian Shiping b, *, Lui Hongxia a, Zhao Jie a, Cao Jiankang a, Li Yongcai a, Zhang Weiyi c
Postharvest Biology and Technology 29(2003)229-232,-0001,():
-1年11月30日
Among three Hami melon (Cucumis melo L. var. inodorus Jacq.) cultivars (Kalakusai, 8601 and New Queen), there were clear differences in sensitivity to chilling injury (CI) during storage at 22 and 1℃. After 10 weeks storage and 1 week shelf life at 1℃, CI was high in Kalakusai fruit. At 3℃, 8601 fruit developed CI after 7 weeks storage and 1 week shelf life. At 5℃, CI was observed in New Queen fruit after 3 weeks storage and 1 week shelf life. Storage for 10 weeks at 3℃, 7 weeks at 5℃, and 3 weeks at 7℃ resulted in the lowest incidence of decay and the highest percentage of marketable fruit for Kalakusai, 8601 and New Queen melons, respectively. Principal fungi isolated from decayed fruit were Fusarium spp., Alternaria spp., Rhizopus spp., Mucor mucedo and Trichothecium roseum. The fruit held at lower temperature had better appearance, less browning and were firmer.
Hami melon, Chilling injury (, CI), , Decay, Quality, Storage temperature
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毕阳, Hongxia Liua, Weibo Jianga, *, Yang Bib, Yunbo Luoa
Postharvest Biology and Technology 35(2005)263-269,-0001,():
-1年11月30日
To investigate how benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) may affect disease resistance of peach fruit (Prunus persica L. cv. Jiubao), harvested fruit were treated with 200 mg/L BTH solution for 5 min immediately after harvest, incubated at 22 ?C and 85-95% RH for 60 h, and then inoculated with Penicillium expansum at a concentration of 1.2×104 conidia per mL. The lesion area and disease-incidence of the BTH-treated fruit were 64.1 and 49.5%, respectively, lower than that of the fruit without BTH-treatment on the 7th day after the inoculation. In the inoculated fruit, the BTH-treatment enhanced the activities of phenylalanine ammonialyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) as well as the levels of total phenolic compounds and hydrogen peroxide (H2O2), which are considered to play important roles in plant disease resistance. Superoxide dismutase (SOD) activity and ascorbic acid (Vc) level in the inoculated fruit were also enhanced by the BTH treatment during the middle and later periods of the inoculation. The results showed that BTH treatment could significantly enhance the disease resistance of peach fruit after harvest and suggest that postharvest treatment with BTH holds promise as a new technology, substituting for chemical fungicide control of postharvest diseases in fruit.
Peach, Disease resistance, BTH, Systemic acquired resistance, Postharvest
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毕阳, Bi Yang a, b, Tian Shiping b, *, Zhao Jie c, Ge Yonghong c
Postharvest Biology and Technology 38(2005)183-187,-0001,():
-1年11月30日
Postharvest application of harpin to induce resistance was studied in two cultivars of Hami melon (Cucumis melo L. var. inodorus Jacq.) inoculated with Trichothecium roseum. Harpin significantly reduced lesion diameter in inoculated fruit. A greater level of decay control was observed in long-term storage cultivars (cv. 8601). The treatment at 90 mg/L was the most effective concentration and higher concentrations over 90 mg/L failed to promote resistance and did not cause phytotoxicity to melons. Harpin did not demonstrate any fungicide effect in vitro, but suppressed lesion diameter in treated and untreated halves of the same fruit, suggesting induction of local and systemic resistance. Efficacy of suppression lasted 8 and 6 days for '8601' and 5 and 3 days for 'New Queen' cultivars in harpin-treated and untreated halves, respectively. The protection by harpin was associated with the activation of peroxidase (POD) and chitinase (CHT).
Harpin, Hami melons (, Cucumis melo L., var., inodorus Jacq., ), , Trichothecium roseum, Induced resistance
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毕阳, WANG Yi*, LI Xuanl, BI Yang, GE Yong-hong, LI Yong-cai and XIE Fang
Agricultural Sciences in China 2008, 7 (2): 217-223,-0001,():
-1年11月30日
Induced resistance was studied in muskmelons (cv. Yindi) inoculated Trichothecium roseurn with postharvest 1,2,3-benzothiadiazole-7-carbothioica cid S-methyl ester (ASM) (100 mg L-1) or harpin (50 mg L-1) treatment. Both ASM and harpin significantly reduced lesion diameter in inoculated fruit. Lesion diameter was limited in the treated and untreated halves of the same fruit, indicating that the local and systemic resistance was induced. Inhibiting efficacy of elicitors lasted 7 and 5 days in the treated and untreated halves. The resistance increased by the chemicals was associated with the activation of peroxidase (POD) and chitinase (CHT). The elicitors induced a significantly and progressively increasing activity of POD and CHT in the treated and untreated halves, and the activation lasted at least 5 days. The activities of POD isoenzymes increased in the treated fruit. However, no new enzyme band was found in the treated and untreated halves.
muskmelon, elicitor, decay, induced resistance
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【期刊论文】采前苯丙噻重氮处理抑制厚皮甜瓜采后挥发性物质的释放
毕阳, 蒋玉梅, 李轩, 毕阳*, 周小平, 周围, 葛永红
农业工程学报,2007,23(3):243-247,-0001,():
-1年11月30日
用50mg/L苯丙噻重氮(BTH)于采前1周喷洒“银帝”甜瓜植株和果实,采收后采用固相微萃取(SPME)结合气-质联用(GC-MS)技术分析果实常温贮藏期间挥发性物质的释放变化。结果表明:BTH处理可明显抑制果实挥发性物质的释放,处理者挥发性物质的释放随贮藏时间的延长而逐渐增加,而对照果实挥发性物质的释放呈单峰形变化,至采后第7d达到高峰。BTH处理者挥发性物质的释放总量也明显低于对照。此外,BTH处理对10种甜瓜特征成分的释放也存在不同程度的影响。
甜瓜, 诱抗剂, 挥发性物质
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【期刊论文】苯丙塞重氮结合枯草芽孢杆菌B1处理对甜瓜 采后主要病害的抑制效果
毕阳, 葛永红, 毕阳*
食品科学,2008,29(6):428-432,-0001,():
-1年11月30日
研究了枯草芽孢杆菌B1(Bacillas subtilis)结合苯丙塞重氮(BTH)处理或它们单独使用对甜瓜采后主要病害的生物防治效果。结果表明:所有处理都降低了Alternaria alternata、Rhizopus stolonifer、Fusarium semitectum的发病率。对于Thrichothecium roseum,B1 结合BTH 和B1 单独处理明显降低了病斑直径;对于A.alternata,BTH 单独处理和结合使用的效果较好;对于R.stolonifer,B1 结合BTH 和B1 单独处理的效果较好;对F.semitectum, 结合使用的效果较好。研究结果还显示,B T H、B 1及结合处理均能诱导甜瓜的过氧化物酶(POD)活性和几丁质酶 (CHT)活性的升高,并且可以持续8d。
采后病害, 生物防治, POD, CHT
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毕阳, Bi, Y., Tian, S. P., Guo, Y. R., Ge, Y. H., and Qin, G. Z.
Plant Disease/March 2006 279,-0001,():
-1年11月30日
The effect of sodium silicate (Si) for control of decay was tested in Hami melons (Cucumis melo L. var. inodorus Jacq.). Si significantly inhibited mycelial growth of Alternaria alternata, Fusarium semitectum, and Trichothecium roseum in vitro. Si at 100 mM was more effective than Si at 25 or 50 mM at controlling the diseases caused by the three pathogens, whereas Si at 200 mM was phytotoxic. Si treatments applied at 100 mM pre-inoculation with T. roseum had lower decay incidence and severity than treatments applied post-inoculation. The protection of Si was correlated with the activation of two families of defense-related enzymes, peroxidase and chitinase. Accumulation of both enzymes was induced in fruit treated with Si and challenged by T. roseum 24 h later, and was sustained for at least 9 days in 'New Queen' and 10 days in '8601' at room temperature. It appeared that induced resistance was an important mechanism of disease control in Hami melons treated with Si.
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【期刊论文】采后苹果酸处理对Penicillium expansum损伤接种梨果实抗病性及活性氧代谢的影响
毕阳, 高晓辉, *, 张怀予, 李小华, 张海霞
食品工业科技,2009,30(7):283-286,-0001,():
-1年11月30日
研究了采后苹果醛浸泡处理对梨果实损伤接种扩展青霉(Penicillium epansum)的抑制以及活性氧代谢和抗氧保护体系的影响。结果表明:离体条件下,50、100、200mmol/L苹果醛处理对P.expansum菌落的生长均有明显的抑制作用,抑苗牢达到59%-64%,但3个处理浓度之间无显著差异。同样,上述3个浓度处理也明显抑制了处理后果实损伤接种P.expansum的病斑直径扩展,表明苹果醛处理诱导了果实体内的抗病陛。苹果醛处理对果实抗病陛的诱导与其影响细胞膜脂过氧化和活性氧代谢有关,处理果实丙二醛(MDA)含量、过氧化氢(H2O2)、超氧阴离子(O2-)和羟基自由基(.OH)水平被明显抑制。同时,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性被明显诱导,谷胱甘肽(GSH)含量也显著上升。P.expansum损伤接种可进一步提高处理果实的H2O2O-2和OH水平,增强SOD和POD活性,降低NDA含量。
苹果酸, 梨, 青霉病, 活性氧代谢, 诱导抗性
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【期刊论文】诱抗剂处理对“银帝”甜瓜采后粉霉病和黑斑病的抑制效果
毕阳, 葛永, 毕阳*, 杨冬梅
食品科学,2006,27(1):246-249,-0001,():
-1年11月30日
采前1d苯丙塞重氮(BTH)或康壮素(Harpin)处理,采后水杨酸(SA)处理可明显降低甜瓜果实采后损伤接种粉红单端孢(Trichothecium roseum)和交链孢(Alternaria alternata)的病斑直径,并且随着诱抗剂浓度的升高其效果越好,其中以100mg/L BTH 或60mg/L Harpin,0.4mg/L SA 处理的效果最佳。
厚皮甜瓜, 苯丙塞重氮, 康壮素, 水杨酸
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