王小菁
1)植物生长发育的光与激素信号转导;2)花卉生长调控
个性化签名
- 姓名:王小菁
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
植物学
- 研究兴趣:1)植物生长发育的光与激素信号转导;2)花卉生长调控
王小菁教授,1981年陕西师范大学获学士学位,1984年西北大学获硕士学位,1990年华南师范大学获博士学位,曾在加拿大多伦多大学植物系做访问学者,在日本大阪市立大学理学部植物园做JSPS博士后研究。担任植物学专业博士生导师、广东省植物发育生物工程实验室主任;中国植物生理学会理事、分子生物学及生物技术专业委员会副主任委员;广东省第三届、第四届学位委员会委员;国家自然科学基金委员会第十、第十一届生命科学部专家评审组成员;教育部高等学校生物科学与工程教学指导委员会生物科学专业教学指导分委员会委员;《植物学报》副主编。
长期从事植物生长发育的调控机理研究,目前研究方向:1)植物生长发育的光与激素信号转导;2)花卉生长调控。曾多次主持和参加国家自然科学基金重点项目和面上项目、教育部回国人员科研启动基金、教育部资助优秀年轻教师基金、教育部骨干教师基金、广东省自然科学基金团队项目等的研究,研究成果曾获得广东省自然科学三等奖(2000)。至今发表论文80余篇,其中在“Plant Physiology”,“Plant Cell Physiology”“植物学报”等国际国内权威刊物发表论文20余篇。近年来,在研究花卉生长发育的细胞和分子机理和调控机制方面发表论文10余篇,获批专利3项。2003年获得全国留学回国人员成就奖,2005年获得国务院政府特殊津贴。参与本科生《生物科学研究方法》《发育生物学》、研究生《植物发育的分子生物学》《细胞信号转导》课程教学工作。
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7
【期刊论文】钙在IAA诱导绿豆下胚轴原生质体膨大过程中的作用*
王小菁, 李德红**, 潘瑞炽
实验生物学报,1999,32(1):55~61,-0001,():
-1年11月30日
含钙培养液(对照)和位用IAA处理的原生质体的体积和45Ca2+放射性强度均无变化。IAA处理含钙培养液中的原生质体。5min后45Ca2+积累明显增多,体积开始膨大。处理30min时45Ca2+积累最多,此时原生质体的膨大效应最好,随后45Ca2+积累和膨大效应逐渐下降。K+、2n2+、Ba2+、Mg2+等也可在一定程度上代替Ca2+使原生质体体积膨大。原生质体的吸水在膨大中起着一定作用。EGTA、LaCl3和verapamil均抑制IAA诱导的原生质体45Ca2+积累和体积膨大。说明Ca2+可能在6BA诱导原生质体嘭大的过程中起着重要作用。
IAA,, 钙 绿豆,, 下胚轴,, 原生质体膨大
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王小菁, 钟康荣, 黄丹, 朱文峰, 高卫东
化学研究,2009,20(2):65~68,-0001,():
-1年11月30日
研究了超声技术促进蔗糖的酯化反应,考察了反应温度、时问、催化剂等对反应的影响。当蔗糖与辛酸乙酯的摩尔比为2∶1,反应温度为70℃,催化剂K2CO3占辛酸乙酯质量分数的11%和反应压力为11kPa的条件下,超声反应2h,能高产率地获得辛酸蔗糖酯,且产物的单酯化物含量达92%。产物结构用红外和核磁表征,产物易溶于极性溶剂,热稳定性好,亲水亲油平衡值(HLB)为10.8。
辛酸蔗糖酯, 廉糖, 辛酸乙酯, 酯交换反应, 超声辐射, HLB
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【期刊论文】蓝光对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响
王小菁, 王晓明*, 王小菁*, 潘瑞炽**
植物生理学报,1999,25(4):321~326,-0001,():
-1年11月30日
蓝光,白光和黑暗叶绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响不同。培养后3-18d,蓝光处理材料的可溶性蛋白质含量明显高于白光处理,更高于黑暗培养的材料。蓝光和白光明显促进3H-亮氨酸掺入蛋白质,而蓝光和白光处理后游离氨基酸含量与黑暗对照相比,下降时间早,幅度太。在培养过程中,蛋白酶活性的变化与游离氨基酸相似。蛋白质合成抑制荆环己酰亚胺(CHM)抑制愈伤组织生长,其中以蓝光最大,白光次之,黑暗最小。在培养基中加入CHM愈早,抑制程度愈大。实验表明,CHM抑制愈伤组织蛋白质合成,也是以蓝光最甚。由此可见,蓝光促进绿豆下胚轴愈伤组织的形成、生长和蛋白质合成,低蛋白质含量,同时抑制细胞脱分化。此外,在绿豆子叶愈伤组织形成过程中,代表蛋白质合成能力的多聚核糖体相对量在子叶切段离体培养的第5天和第7天增加到原来的3倍多,表明蛋白质合成能力迅速增强(谭保才等1993)。然而,这些组织培养研究均未涉及光质的影响。本实验以不同光质处理绿豆下胚轴切段,研究愈伤组织形成和生长过程中蛋白质含量、蛋白质酶活性及蛋白质合成的动态变化,以此了解蓝光影响其蛋白质代谢的特征。
绿豆,, 蓝光,, 愈伤组织,, 蛋白质
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【期刊论文】单克隆抗体ELISA测定UV-B诱导的DNA损伤*
王小菁, 李韵山a, 王艳, 宾金华b, 王小菁b, 刘颂豪
激光生物学报,1999,8(1):26~29,-0001,():
-1年11月30日
本文采用单克隆抗体酶联免疫吸附分析法测定了UV-B诱导DNA产生的CPD和6-4PP。经0.5mW/cm2UV-B处理15min的小牛胸腺和鲱鱼精DNA,CPD和6-4PP含量显著增加,而未经UV-B处理的对照DNA则没有二聚体形成。
UV-B辐射, DNA损伤, 环丁烷嘧啶二聚体(, CPD), , 6,, 4-光产物(, 6-4PP), , 酶联免疫吸附分析法(, ELISA),
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王小菁, Xiaojing Wang and Moritoshi Iino*
Plant Physiol.(1998) 117: 1265-1279,-0001,():
-1年11月30日
Protoplasts isolated from red-light-adapted Arabidopsis hypocotyls and incubated under red light exhibited rapid and transient shrinking within a period of 20 min in response to a blue-light pulse and following the onset of continuous blue light. Long-persisting shrinkage was also observed during continuous stimulation. Protoplasts from a hy4 mutant and the phytochrome-deficient phyA/phyB double mutant of Arabidopsis showed little response, whereas those from phyA and phyB mutants showed a partial response. It is concluded that the shrinking response itself is mediated by the HY4 gene product, cryptochrome 1, whereas the blue-light responsiveness is strictly controlled by phytochromes A and B, with a greater contribution by phytochrome B. It is shown further that the far-redabsorbing form of phytochrome (Pfr) was not required during or after, but was required before blue-light perception. Furthermore, a component that directly determines the blue-light responsiveness was generated by Pfr after a lag of 15 min over a 15-min period and decayed with similar kinetics after removal of Pfr by far-red light. The anion-channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid prevented the shrinking response. This result, together with those in the literature and the kinetic features of shrinking, suggests that anion channels are activated first, and outward-rectifying cation channels are subsequently activated, resulting in continued net effluxes of Cl2 and K1. The postshrinking volume recovery is achieved by K1 and Cl2 influxes, with contribution by the proton motive force. External Ca21 has no role in shrinking and the recovery. The gradual swelling of protoplasts that prevails under background red light is shown to be a phytochrome-mediated response in which phytochrome A contributes more than phytochrome B.
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【期刊论文】钙在6-BA诱导黄化绿豆幼苗下胚轴原生质体体积膨大中的作用*
王小菁, 李德红**王小菁, 潘瑞炽
实验生物学报,1998,31(2):187~193,-0001,():
-1年11月30日
This paper studied on the role of calcium in 6-BA-induced swelling of protoplasts isolated from hypocotyl in etiolated mung bean(Phaseolus radiatus L.) seedlig. Protoplasts incubated in CaCl2-bearing medium without hormone maintained a constant volume. To treat with 6-BA, they began to swell after 15 minutes, and continually swelled to the maximum volume 30 minutes later when they were treated with 10-1 mol/L 6-BA(Fig.2). However, the protoplasts could not swell when 6-BA was added into the medium without CaCl2(Fig.1). It was suggested that Ca2+ was necessary for 6-BA to induce protoplast swelling. 6-BA enabled the protoplasts to swell in less extent when K-. Zn2+, Ba2+or Mg2-instedad of Ca2-(Fig.3). Radioisotope experiments showed that K+influx incrased when Ca2+ as replaced by K+(Fig.4). Ca2+ accumulation in protoplasis treated by 6-BA was munch higher than that of control, and the time course of Ca2+ accumu-lation was similar to protoplasts swelling (Fig5). 45Ca2+level and the swelling of protoplasis were sharply declined when EGTA, verapamil or LaCcl3 was added into the medium(Table 1,2and3). These results indiated that Ca2+ may play an important role in 6-BA-induced swelling.
6-BA., Calcium., Mung bean seedling., Hypocotyl., Protoplast welling.,
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王小菁, Xiaojing, Wang and Moritoshi lino*
Plant Physiol.(1997) 114: 1009-1020,-0001,():
-1年11月30日
Protoplasts isolated from red-light-grown maize (Zea mays L.) coleoptiles shrank transiently upon brief exposure (e.g. 30 s) to blue light under background irradiation with red light. The maximal volume reduction (about 4% at a saturating fluence) occurred about 5 min after blue-light stimulation. The response was prevented by the anion-channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid. Red light and far-red light did not induce any comparable response. Protoplasts of different sizes and those isolated from different coleoptile positions showed similar responses. After treatment with a saturating blue-light pulse, the protoplasts became responsive to a second pulse and gained full responsiveness within 5 min, suggesting that the photoreceptor system involves a dark-reversible component. The response to continuous blue light was also found to be transient. The protoplast volume was reduced during about 6 to 9 min of irradiation and returned within the next 30 min to the control level. The response to continuous blue light was saturated at 30 pmol m-2s-1. However, when the fluence rate was enhanced 10-fold after a period of irradiation at 30 pmol m-2s-1, the protoplasts showed another shrinking response. These and other kinetic results indicate that the photoreceptor system undergoes a photosensory adaptation. Crowth in different zones of the coleoptile was inhibited by blue light transiently after pulse stimulation, as well as during continuous stimulation. It was concluded that the observed protoplast shrinking is related to the blue-light-induced inhibition of coleoptile growth.
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