黄民
博士研究生 教授 博士生导师
中山大学 临床药理研究所
临床药理学;药代动力学
暂无
- 姓名:黄民
- 目前身份:在职研究人员
- 担任导师情况:博士生导师
- 学位:
-
学术头衔:
享受国务院特殊津贴专家
- 职称:高级-教授
-
学科领域:
细胞生物学
- 研究兴趣:临床药理学;药代动力学
黄民,男,1963年10月生,二级教授,博士生导师。中山大学临床药理研究所所长,享受国务院政府特殊津贴专家。教育经历:1979.9—1983.6 上海医科大学本科学习,获学士学位; 1985.9--1988.6 中山医科大学药理学研究生,获硕士学位; 1994.10—1998.8 香港大学获药理学博士研究生。工作经历:1983.6—1989.11 中山医科大学临床药理教研室助教; 1989.12—1994.11 中山医科大学临床药理教研室讲师; 1994.12—2000.11 中山医科大学临床药理教研室副教授; 2002.6—2016.08 中山大学药学院常务副院长、院长;2000.12 — 现在 中山大学临床药理研究所教授、所长。
研究方向:
临床药理学、药代动力学。近年来对相关药物代谢酶和转运蛋白及其上游调控基因的基因多态性及其与临床药物疗效及不良反应相关性有较为深入的研究;同时在体外细胞、分子水平,在体或离体器官灌流水平,计算机模拟水平发展了一系列的药代动力学技术模型,并在中药药物代谢相互作用及其调控机制、临床药物相互作用预测、临床合理用药方面有较深入的探讨。
近年来对相关药物代谢酶和转运蛋白及其上游调控基因的基因多态性及其与临床药物疗效及不良反应相关性有较为深入的研究;同时在体外细胞、分子水平,在体或离体器官灌流水平,计算机模拟水平发展了一系列的药代动力学技术模型,并在中药药物代谢相互作用及其调控机制、临床药物相互作用预测、临床合理用药方面有较深入的探讨。在国内外重要学术刊物,包括Curr Drug Metab, J Pharmacol Exp Ther, Drug Metab Dispos, Pharm Res, Br J Clin Pharmacol,Eur J Pharm Sci, Int Immunopharmacol, Clin Chim Acta, Clin Pharmacokinet, J Mass Spectrom, Rapid Commun Mass Spectrom, J Pharm Biomed Anal, J Chromatogr B, Xenobiotica和Invest New Drugs等发表论文100余篇(其中被SCI收录90余篇),主编或参与出版了有关药动学专著和教材9部;已承担国家“重大新药创制”重大专项课题、国家自然科学基金、教育部985重点建设专项、省部重大项目等20多项的研究课题;作为负责人已主持完成近百个药物(其中不少属于国家一类、二类、三类新药)的非临床、临床评价工作。
社会/学术任职:
担任国家药典委员、国务院学位委员会第七届药学学科评议组成员、中国药理学会常务理事、临床药理专业委员会主任委员、药物代谢专业委员会委员(理事)、International Society for the Study of Xenobiotics(ISSX)财经委员会委员、广东省科协常委、广东省药理学会名誉理事长、广东省药学会副理事长、广东省药物评价及合理用药专业委员会主任委员;“Drug Metabolism and Disposition ”、“中国药学英文版”、 “药学学报”、“中国临床药理学杂志”、“中国医药工业杂志”、“中药新药与临床药理” 、“中国临床药理学与治疗学杂志”、“中国药学杂志英文版”、“今日药学”杂志编委;国家食品药品监督管理总局药品审评专家库专家、广东省药品审评专家库专家。
-
主页访问
2868
-
关注数
0
-
成果阅读
922
-
成果数
12
黄民, MIN HUANG, *, †, JIPING LI, ‡, HWEE TEOH, and RICKY Y. K. MAN*
,-0001,():
-1年11月30日
Micromolar concentrations of estradiol are required to inhibit the oxidation of low-density lipoproteins (LDL) in vitro. Recent evidence suggests that estradiol must be modified before it can become an effective antioxidant at physiological levels. Our aim was to determine other possible conditions under which low concentrations of 17b-estradiol can reduce LDL oxidation. LDL susceptibility to oxidation was monitored by measurements of conjugated diene formation. High levels of 17b-estradiol reduced oxidative modification of LDL. Vitamin C and vitamin E also increased LDL resistance to Cu21-mediated oxidation. More importantly, 10 nM 17b-estradiol, which on its own had no effect, exhibited significant antioxidant actions in the presence of either vitamins C or E. In conclusion, supraphysiological concentrations of 17b-estradiol are required to exert antioxidant effects directly in vitro. However, in the presence of vitamins C and E, concentrations of 17b-estradiol close to physiological levels can also protect LDL from oxidation.
Estradiol,, Lipoproteins,, Antioxidants,, Vitamin C,, Vitamin E,, Lipid peroxidation,, Free radicals
-
64浏览
-
0点赞
-
0收藏
-
0分享
-
113下载
-
0评论
-
引用
黄民, Shi-long Zhonga, Shufeng Zhoub, *, Xiao Chenc, Min Huanga
Clinica Chimica Acta xx (2005) xxx-xxx,-0001,():
-1年11月30日
Background: The glutathione S-transferase (GST) superfamily comprises multiple isozymes with compelling evidence of functional polymorphisms in various ethnic groups. All these mutations, in particular those in class A, k and u GST, are likely to contribute to interindividual differences in responses to xenobiotics including response to chemotherapy and associated with altered disease. The frequency of common GST mutations in Uygur Chinese is unknown. We investigated the common mutations of GSTM1, GSTT1, and GSTP1 in Uygur (N =154) Chinese and compare with Han Chinese (N =196). Method: GSTM1 and GSTT1 polymorphisms were analyzed by multiplexed PCR, and GSTP1 polymorphism was detected by PCR-based restriction fragment length polymorphism (RFLP) analysis. Results: GSTM1 null genotype was found in 53.2% Uygur Chinese, which was close to that in Han Chinese (56.1%) ( P=0.592). A significantly lower frequency ( P <0.05) of GSTT1 null genotype in Uygur Chinese (26.6%) was observed compared with Han Chinese (50.0%). Uygur Chinese exhibited a GSTP1 genotype distribution of 51.3% I/I, 40.2% I/V and 8.4% V/V, which was different from that in Han Chinese (60.7% I/I, 35.2% I/V and 4.1% V/V). Conclusions: There is marked ethnic difference in the frequency of common GSTT1 and GSTP1 mutation, but not GSTM1 mutation, between Uygur and Han Chinese. D 2005 Elsevier B.V. All rights reserved.
Glutathione S-transferase, Mutation, PCR, Uygur Chinese, Han Chinese
-
103浏览
-
0点赞
-
0收藏
-
0分享
-
150下载
-
0评论
-
引用
【期刊论文】Prediction of Herb-Drug Metabolic Interactions: A Simulation Study
黄民, Shufeng Zhou*, Min Huang, Anlong Xu, Hongyuan Yang, Wei Duan and James W. Paxton
Phytother. Res. 19, 464-471 (2005),-0001,():
-1年11月30日
In vitro and in vivo studies have indicated that the induction or inhibition of cytochrome P450 (CYP) is one of the major mechanisms for some clinically important pharmacokinetic herb-drug interactions. An attempt was made to simulate the effects of herbal preparation with single or multiple CYP-inhibiting constituents on the area of the plasma concentration-time curve (AUC) of coadministered drug that was either a low clearance drug by intravenous (i.v.) injection or a high clearance drug by oral route. Our simulation studies indicated that the expected increase (Rc) in the AUC of the coadministered drug by inhibiting herbal constituent(s) was dependent on the route of administration. For low clearance drug by i.v. injection, Rc was generally determined by inhibition constant (Ki), unbound inhibitor concentration ([I]), hepatic fraction (fh), number of inhibitory herbal constituents (n) and metabolic pathway fraction in hepatic metabolism (fm), while Rc for a high clearance drug by oral route, Rc was determined by Ki, [I], n and fm. By varying these parameters, Rc changed accordingly. It appeared likely to predict a herb-drug metabolic interaction, if the inhibiting herbal constituents could be quantitatively determined. However, many herb- and drug-related factors may cause difficulties with the prediction, and thus in vivo animal and human studies are always necessary. Copyright
herb, metabolic inhibition, cytochrome P450, drug interactions.,
-
82浏览
-
0点赞
-
0收藏
-
0分享
-
200下载
-
0评论
-
引用
-
100浏览
-
0点赞
-
0收藏
-
0分享
-
529下载
-
0评论
-
引用
黄民, Hui-chang Bi†, Guo-ping Zhong†, Shufeng Zhou*, Xiao Chen, and Min Huang**
Rapid Commun. Mass Spectrom. 2005; 19: 2911-2917,-0001,():
-1年11月30日
A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated to determine the concentrations of adefovir [9-(2-phosphonylmethoxyethyl)adenine, PMEA] in human plasma. After one-step protein precipitation of plasma samples by methanol, adefovir was analyzed by LC/MS/MS using positive electrospray ionization. Chromatography was performed on a C18 column. The extraction recoveries of adefovir were found to be 85.1-89.3%. Adefovir was stable under routine laboratory conditions. A minimal matrix effect resulting in a slight ionization enhancement of adefovir (<10.9%) was observed, which did not markedly affect the behavior of the calibrations curves and accuracy and precision data. The method had a chromatographic run time of 7.8min and a linear calibration curve over the concentration range 1.5-90ng/mL for adefovir. The lower limit of quantification of the method was 1.5ng/mL. The intra-and inter-day precision was less than 8.4%. These results indicated that this LC/MS/MS method has high selectivity and efficiency, and acceptable accuracy, precision and sensitivity. The validated LC/MS/MS method has been successfully used in a pharmacokinetic study in healthy volunteers treated with oral adefovir dipivoxil at 10 and 20mg.
-
82浏览
-
0点赞
-
0收藏
-
0分享
-
291下载
-
0评论
-
引用
【期刊论文】St. John’s Wort Modulates the Toxicities and Pharmacokinetics of CPT-11 (Irinotecan) in Rats
黄民
,-0001,():
-1年11月30日
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
79下载
-
0评论
-
引用
【期刊论文】Human Multidrug Resistance Associated Protein 4 Confers Resistance to Camptothecins
黄民, Quan Tian, Jing Zhang, , Theresa May Chin Tan, Eli Chan, Wei Duan, Sui Yung Chan, Urs Alex Boelsterli, Paul Chi-Lui Ho, Hongyuan Yang, Jin-Song Bian, Min Huang, Yi-Zhun Zhu, Weiping Xiong, Xiaotian Li, and Shufeng Zhou
Pharmaceutical Research (2005),-0001,():
-1年11月30日
Purpose. The multidrug resistance associated protein (MRP) 4 is a member of the adenosine riphosphate (ATP)-binding cassette transporter family. Camptothecins (CPTs) have shown substantial anticancer activity against a broad spectrum of tumors by inhibiting DNA topoisomerase I, but tumor resistance is one of the major reasons for therapeutic failure. Pglycoprotein, breast cancer resistance protein, MRP1, and MRP2 have been implicated in resistance to various CPTs including CPT-11 (irinotecan), SN-38 (the active metabolite of CPT-11), and topotecan. In this study, we explored the resistance profiles and intracellular accumulation of a panel of CPTs including CPT, CPT-11, SN-38, rubitecan, and 10-hydroxy-CPT (10-OH-CPT) in HepG2 cells with stably overexpressed human MRP4. Other anticancer agents such as paclitaxel, cyclophosphamide, and carboplatin were also included. Methods. HepG2 cells were transfected with an empty vehicle plasmid (V/HepG2) or human MRP4 (MRP4/HepG2). The resistance profiles of test drugs in exponentially growing V/HepG2 and MRP4/HepG2 cells were examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazonium bromide (MTT) assay with 4 or 48 h exposure time of the test drug in the absence or presence of various MRP4 inhibitors. The accumulation of CPT-11, SN-38, and paclitaxel by V/HepG2 and MRP4/HepG2 cells was determined by validated high-performance liquid chromatography methods. Results. Based on the resistance folds from the MTT assay with 48 h exposure time of the test drug, MRP4 conferred resistance to CPTs tested in the order 10-OH-CPT (14.21) >SN-38 carboxylate (9.70) >rubitecan (9.06) >SN-38 lactone (8.91) >CPT lactone (7.33) >CPT-11 lactone (5.64) >CPT carboxylate (4.30) >CPT-11 carboxylate (2.68). Overall, overexpression of MRP4 increased the IC50 values 1.78-to 14.21-fold for various CPTs in lactone or carboxylate form. The resistance of MRP4 to various CPTs tested was significantly reversed in the presence of DL-buthionine-(S,R)-sulfoximine (BSO, a gglutamylcysteine synthetase inhibitor), MK571, celecoxib, or diclofenac (all MRP4 inhibitors). In addition, the accumulation of CPT-11 and SN-38 over 120 min in MRP4/HepG2 cells was significantly reduced compared to V/HepG2 cells, whereas the addition of celecoxib, MK571, or BSO significantly increased their accumulation in MRP4/HepG2 cells. There was no significant difference in the intracellular accumulation of paclitaxel in V/HepG2 and MRP4/HepG2 cells, indicating that Pglycoprotein was not involved in the observed resistance to CPTs in this study. MRP4 also conferred resistance to cyclophosphamide and this was partially reversed by BSO. However, MRP4 did not increase resistance to paclitaxel, carboplatin, etoposide (VP-16), 5-fluorouracil, and cyclosporine. Conclusions. Human MRP4 rendered significant resistance to cyclophosphamide, CPT, CPT-11, SN-38, rubitecan, and 10-OH-CPT. CPT-11 and SN-38 are substrates for MRP4. Further studies are needed to explore the role of MRP4 in resistance, toxicity, and pharmacokinetics of CPTs and cyclophosphamide.
camptothecin, cyclophosphamide, drug transporter, multidrug resistance.,
-
102浏览
-
0点赞
-
0收藏
-
0分享
-
151下载
-
0评论
-
引用
黄民, Jian-ping Zhang, , Yong-yuan Guan, Jue-heng Wu, An-Long Xu, Shufeng Zhou, & Min Huang
Br J Clin Pharmacol 58: 2, 163-168,-0001,():
-1年11月30日
thiopurine S-methyltransferase,, phenotype,, genotype,, TPMT*, 3C
-
77浏览
-
0点赞
-
0收藏
-
0分享
-
126下载
-
0评论
-
引用
【期刊论文】Gene mutation of thiopurine S-methyltransferase in Uygur Chinese
黄民, Jian-Ping Zhang, Yong-Yuan Guan, An-Long Xu, Shu-Feng Zhou, Jue-Heng Wu, Hong Wei, Min Huang
Eur J Clin Pharmacol (2004) 60: 1-3,-0001,():
-1年11月30日
Objective: This study was to investigate the gene mutation of thiopurine S-methyltransferase (TPMT) in Uygur Chinese. Methods: Polymerase chain reaction-based methods were used to analyze three commonly reported inactivating mutations-G238C, G460A and A719G. Results: One TPMT*3A heterozygote and five TPMT*3C heterozygotes were found in 160 Uygur Chinese subjects, and allele frequencies of TPMT*3A and TPMT*3C were 0.3% and 1.6%, respectively. Conclusion: TPMT*3C is a common mutant allele in Uygur Chinese, while TPMT*3A is a rare mutant allele in Uygur Chinese.
Thiopurine
-
74浏览
-
0点赞
-
0收藏
-
0分享
-
60下载
-
0评论
-
引用
黄民, Yihuai Gaoa, Shufeng Zhoub, *, Jianbo Wenc, Min Huangd, Anlong Xue
Life Sciences 72(2002)731-745,-0001,():
-1年11月30日
Many cytokines, in particular tumor necrosis factor (TNF)-a have been known to play an important role in the pathogenesis of gastric mucosal lesions caused by various factors such as drugs and Helicobacter pylori infection. Our previous studies have shown that the polysaccharide fractions isolated from the fruiting bodies of Ganoderma lucidum (GLPS) prevented indomethacin- and acetic acid-induced gastric mucosal lesions in the rat. However, the mechanisms remain unclear. This study aimed to investigate whether GLPS had a direct mucosal healing effect in the indomethacin-treated rat, and to explore the possible mechanisms by determining the gastric mucosal mRNA and protein levels of TNF-a and ornithine decarboxylase (ODC) activity. In addition, the effects of GLPS on the cellular proliferation, ODC and c-Myc protein expression and mucus synthesis in the rat gastric cell culture (RGM-1) were examined. The present study demonstrated that GLPS at 250 and 500mg/kg by intragastric input caused ulcer-healing effect in the rat; this was accompanied with a significant suppression of TNF-a gene expression, but with an increased ODC activity. In RGM-1 cells, GLPS at 0.05, 0.25 and 1.0mg/ml significantly enhanced [3H]thymidine incorporation and ODC activity in a concentration-dependent manner. However, these effects were abrogated by the addition of the ODC inhibitor, DL-a-difluoromethyl-ornithine (DFMO). GLPS at 0.25-1.0mg/ml also increased mucus synthesis, as indicated by the increased D-[6-3H]glucosamine incorporation in RGM-1
-
70浏览
-
0点赞
-
0收藏
-
0分享
-
85下载
-
0评论
-
引用