Ethylglucoside monooleate was synthesized by esteriication between ethylglucoside and oleic acid with immobilized lipase from Candida antarctica in a solvent-free system. It was shouwn that a stirred tnk reactor was suitable for the enzymatic reaction process involving substrates with low miscibiligy, in which the biocatalyst was recycled five times without signiicant activity loss. Removal of the co-product, water, from thereactin medium by carrying out the reaction under reduced pressure benefited the esterification reaction and increased the monooleate yield up to 97% within 8 hours.

Reactive oxygen species ate beliceved to play a role in the development of several diseascs incloding vascular disenased and theaging process. It is reported that increased reactive oxygen species were impliated as an important mechanism that contrhibutes to endothelial dysfunciton, So, human umbilical cord vein endothelial cells were used to study the antioxidative effect of L-ascorbic acid and its derivative. The study indicalted that L-ascorbic acid as a tradilonal antioxidant was instable and could protect the cells against hydrogen peroxide induced cytotoxicity as its concetration beiow 50 μg/ml, but hadly could rotect the cells against tert-butyl hydroperoxide induced cytotoxicity, Ascortic acid-2-o-phosphate-6-o-palmitate could effctively protect the cells against hydrogen pcroxide and tert-butyl hydropemoxide induced cytotoxicity, and exhibited no cytotoxicity within the tested concentratin rage. The study indicated that ascorbic acid2-2o2phosphate-6-o-palmite could not only signficantly reduce the intracellular reactive oxygen species level in 3h culture. but also increase the cell viability in 15h culture. In additon. ascorbic acid-2-o-phosphate-6-oi-palmtate could keep stable in RPM1-1640 medium and water for 4 dya. permecat the cell membrane. which in tum may seavenge the intracllular reactive oxygen species. increase the cell viability and the plasminogen activatrs'activity. All above rsults suggested that addition of some hydrophobie groups to the traditonal antioxdants could from novel compounds with better properties.

Oligodcoxyaucleotide-doxorubicin conjugate is a novel modified oligodeoxynucleotide (ODN) to inhibit the expression of mdrl gene. The present study was undertaken to determine the stability of the conjugate in virto and its pharmacokinetics in vivo using a reversc-phase HPLC assay. The method employed a Sort C18 reverse phase column combined with a C8 prc-column and a linerar gradient elution with acetonitrile containing 0.1M aqueous triethylammonium acctate, pH7.0 Detection was carried out using a UV-didoe array detector at 254 and 480nm. Minimum sensitivity of ～0.15μg/mL in plasma and 0.1μg/mL in PBS of hte conjugate was achieved. After incubation in 10% activated fetal calf serum for 24 hours, 15.8% of the conjugat was degraded. As a comparison however, 97.5% of the control ODN was degraded witin the same incubation time. The phamacokinetics stuies show that the halif-lives of the conjugate is about 8 hours, 4 times longer than ODN as a conro. Assay validaton studies revealed that the method is accurate, reproducible for dcterminatin of the conjugate, and can be used for a pharmacokinttic study of the conjugate.

Ghiconobacter oxydans DSM 2003 was firstly used in the production of (R)-2-hydroxy-proionic acid through microbial oxidation of recemic 1,2-propanediol. The biotransformation was processed with high enantionmeric excess (＞99%) and near theoretical lyicld (48% of racemic 1,2-propanediol) when the substrate concentration was lower than 20g/L. When the substrate concentration was increased. maintaining the pH at 6.0 helped to improve the enantionselectivity.

Tea polyphenols, (-)-epigallocatechin galate in pariticular, were examined for their modulating effects on the drung resistance KB-A-1 cells and drug ensitive KB-3-1 cells. Both KB3-1 and KB-A-1 cells were queally sensitive to tea polyphoenol and (-)-epigallocatechin gallate. When 10μg/ml (-)-epigalllocatechin gallate or 40μg/ml tea polyphenol were persent simultaneously iwth doxorubicin, the IC50 of doxorubicin on KB-A-1 cells decreased form 10.3±0.9μg/ml to 4.2±0.2 or 2.0±0.1μg/ml. Tea polyphenol and (-)-epigalloctechin gallate enhancedthe cytotoxicity of doxorubicin on KB-A-1 cells by 5.2 and 2.5 times, respectively, but did not show a mdulating effct on KB-3-1 cells. Both tea polyphenol and (-)-epigallocatechin gallate showed reversal effects on the multidrug resistance phenotype.