The polysaccharide and flavonoid concentrations of two-, three-, and four-year-old Aloe vera were determined, and their antioxidant activities were evaluated compared to BHT and R-tocopherol by the DPPH radical scavenging method and the linoleic acid system at 100

Five compounds oxidizing canine erythrocytes were isolated from an aqueous ethanol garlic extract by sillica gel column chromatograpjhy and preparative thin-layer chromatography. On the basis of nuclear magnetic resonance, infraed spectroscopy, and mass spectrometry, they were identified as three known compounds: bis-2-propenyl trisulfide (1), bis-2-propentl tetrasulifide (2), and bis-2-propenyl pentasulfide (3) as well as two novel compounds, bis-2-propenyl tetrasulifide (4) and transsulfunic acid alyl ester 3-allyisulfanyl-allyl ester (5)-A mixture of compounds 1-3 and compounds 4 and 5 induced methemoglobin formation in canine erythrocyte suspension in vitro resulting in the oxidation of canine erythrocytes. These groups of characteristic organosulfur compounds contained in gralic probably contrbute to oxidations in blodd. The constituents of garlic have the potential to oxidize erythrocytes and hemoglobin, suggesting that foods containing quantities of garlic have the potential to oxidize erythrocytes and hemoglobin, suggesting that foodds containing quantities of garlic should be avoiede for feeding dogs.

activities of extracts derived from sesame seed by supercritical carbon dioxide (SC-CO2) extraction and by n-hexane were determined using R,R-diphenyl-â-picylhydrazyl (DPPH) radical scavenging and linoleic acid system methods. The highest extracted yield was given at 35℃, 40MPa, and a CO2 flow rate of 2.5mL min-1 by an orthogonal experiment. The yields of extracts increased with increasing pressure, and yields at 40 and 30MPa were higher than that by solvent extraction at 46.50%. Results from the linoleic acid system showed that the antioxidant activity follows the order: extract at 3℃, 20MPa>BHT>extract at 55℃, 40MPa>extract at 55℃, 30MPa>Trolox>solvent extraction>R-tocopherol. The SC-CO2 extracts exhibited significantly higher antioxidant activities comparable to that by n-hexane extraction. The extracts at 30MPa presented the highest antioxidant activities assessed in the DPPH method. At 20MPa, the EC50 increased with temperature, which indicated that the antioxidant activity was decreased in a temperature-dependent manner. The significant differences of antioxidant activities were found between the extracts by SCCO2 extraction and n-hexane. However, no significant differences were exhibited among the extracts by SC-CO2 extraction. The vitamin E concentrations were also significantly higher in SC-CO2 extracts than in n-hexane extracts, and its concentrations in extracts corresponded with the antioxidant activity of extracts.

Two experiments were conducted to study the effect of the application of selenium on the selenium content of soybean and its products in two counties with selenium-deficient soil. Selenium-enriched soybean was produced by the application of sodium selenite and Se-enriched fertilizer. The selenium contents of soybeans, soybean protein and okra were determined by hydride-generation atomic fluorescence spectrometry. The results showed that the selenium contents of soybean, soybean protein, and okra were significantly increased by the application of sodium selenite and selenium-enriched fertilizer. Foliar application of selenium provided a higher efficiency for increasing the selenium content of soybean than soil application. Significant differences were found in that soybean cultivars exhibited different accumulation of selenium. There was no remarkable difference in soybean yield, soybean protein, and lipid between selenium and control. The selenium-enriched protein derived from selenium-enriched soybean could be used as a functional ingredient and soybean okra as a selenium-enriched feed for animals for increasing selenium intake.