This is the first report on the determination of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo (Cal-Red) by Rayleigh light scattering (RLS). At pH 4.07, the weak RLS of Cal-Red can be enhanced greatly by the addition of proteins. On this basis, the reaction of Cal-Red and proteins was studied. A new quantitative determination method for proteins has been developed. This method is very sensitive (0:45-36:9lgml 1 for bovine serum albumen (BSA)), rapid (<2 min), simple (one step), and tolerant of most interfering substances. The maximum binding number of Cal-Red to BSA was 143 and the binding constant was 4.1

A new method is proposed for the spectrophotometric determination of Pd(Ⅱ), based on the reaction of Pd(Ⅱ) with 2-(4-chloro-2-phosphonophenylazo)-7-(3-carboxyphenylazo)-1,8-dihydroxynaphthalene-3,6-disulfonic acid(CPA-mK) in sulfuric acid without heating. Beer's law is obeyed for 1.0-4.0 mg of Pd (Ⅱ) in 10 mL of solution. The calibration curve from 1.0 to 42.0mg in 10mL of solution is modeled successfully by artificial neural networks (ANNs). The maximum relative error between experimental values and the values predicted by ANNs is 1.5%. In comparison with some mathematical functions, ANNs show better ability for curve fitting, thus greatly extending the applicable range of the calibration curve of this new system. The method has been applied to determine Pd (Ⅱ) in ore and catalyst samples with a relative error of less than 4% and with a recovery range between 94% and 103%.

A new rapid, selective and sensitive on-line microwave flow injection-kinetic method was developed for spectrophotometric determination of micro amounts of Ir(Ⅲ), based on its catalytic effect on the m-acetylchlorophosphonazo (CPA-mA) and KIO4 reaction in NaOH media. An on-line microwave oven was employed to accelerate the reaction. The reaction was followed spectrophotometrically by measuring the decrease of the absorbance of CPA-mA at 580 nm. The effect of five variables for the determination of Ir(Ⅲ) was optimized by means of a multilayer artificial neural network using extended deltabar-delta (EDBD) algorithms. Under the optimum experimental conditions, Ir(Ⅲ) could be determined in the range 0.060-0.60 μgZZZ; mL-1 with detection limit of 0.02 μgZZZ;mL-1 and the sampling frequency of 34 h-1. The proposed method was applied to the determination of micro amounts of Ir(Ⅲ) in refined ore and secondary alloy with the recoveries from 91.4% to 109%.

A simple high-performance liquid chromatography (HPLC) method is described for the determination of matrine in rat plasma. The plasma was deproteinized with acetonitrile that contained an internal standard (phenacetin) and was separated from the aqueous layer by adding sodium chloride. Matrine was extracted into the acetonitrile layer with high yield, and determined by reversed-phase HPLC (column: YMC-pack ODS-A, 5mm, 150

For the first time, an organic dye, morin, and another bio-macromolecule, human serum albumen (HSA), were embedded in porous alumina (P-Al) fabricated by anodization in oxalic acid. The photoluminescent (PL) spectra of morin and morin-HSA immersed in the P-Al pores were measured and compared with those of liquid solutions. It was found that their PL positions are similar to those of dye-Al3+ in ethanol solution, and the PL of the embedded morin was greatly enhanced by the introduction of HSA. We have ascribed the appearance of PL bands to the formation of morin-Al complexes in the nanopores, with its inner wall being involved. A likely luminescence model was proposed to explain the observed PL enhancement due to the coexistence of morin and HSA in the nanopores, which has been proved by UV and Fourier transform infrared measurements. Moreover, the PL intensity increased with increasing pore diameter, which may be used to develop a luminescent array to predict the pore size of a P-Al layer.