Objective To determine over-expression of a truncated type Ⅱ TGF-β receptor in doum-regulating TGF-β1 autoproduction in normal dermal fibroblasts. Methods In vitro cultured dermal fibro-blasts were treated with rhTGF-β1 (5ng/ml) or recombinant adenovirus containing a truncated type Ⅱ TGF-β receptor gene (50 pfu/cell). Their effects on regulating gene expression TGF-β1 were observed with. Northern Blot. Results rhTGF-β1 up-regulated the gene expression of TGF-β1 (34%～150%) and type I procollagen (13%～190%). Over-expression of a truncated receptor Ⅱ decreased the gene expression of TGF-β1 (53%～66%). Conclusion Over-expression of the truncated TGF-β receptor Ⅱ down-regulated TGF-β1 auto production via blocking signal transduction of TGF-β. This study may provide a new strategy for scar gene therapy.