曹谊林
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- 姓名:曹谊林
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学术头衔:
博士生导师, 国家杰出青年科学基金获得者
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无线电物理
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暂无
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4186
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712
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成果数
14
【期刊论文】Recent advances in tissue engineering of cartilage, bone, and tendon
曹谊林, Wei Liu, Lei Cui and Yilin Cao
Curr Opin Orthop 15: 364-368,-0001,():
-1年11月30日
tissue engineering,, cartilage,, bone,, tendon
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曹谊林, W. Liu*, D.R. Wang and Y.L. Cao
Current Gene Therapy, 2004, 4, 123-136,-0001,():
-1年11月30日
Hypertrophic scar and keloid are common and difficult to treat diseases in plastic surgery. Results of wound healing research over the past decades have demonstrated that transforming growth factor-β (IGF-β) plays an essential role in cutaneous scar formation In contrast, fetal wounds, which heal without scarring, contain a lower level of TGF-β than adult wounds. How to translate the discovery of basic scientific research into the clinical treatment of wound scarring has become an important issue to both clinicians and basic researchers. The development of gene therapy techniques offers the potential to generically modify adult wound healing to a healing process similar to fetal wounds, and thus reduces wound scarring. This article intends to review the roles of TGF-β in the formation of wound scarring, the possible strategies of antagonizing wound TGF-β, and our preliminary results of scar gene therapy, which show that wound searring can be significantly reduced by targeting wound TGF-β.
Wound scarring,, TGF-β,, gene therapy,, adenovirus,, gene transfer
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曹谊林, GAN SHEN*, HSIAO CHIEN TSUNG*, CHUN FANG WU, XIAO YIN LIU, XIAOYUN WANG, WEI LIU, LEI CUI, YI LIN CAO**
Cell research (2003); 13 (5): 335-341,-0001,():
-1年11月30日
Endothelial ceils (TEC3 cells) derived from mouse embryonic stem (ES) cells were used as seed cens to construct blood vessels. Tissue engineered blood vessels were made by seeding 8×106 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the ceI1-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6~8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6~8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelinm lining in tissue engineered blood vessels.
tissue engineering,, embryonic stem cell,, blood vessel,, differentiation,, endothelial cell.,
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曹谊林, WEI LIU, M.D., Ph.D., LEI CUI. M.D., and YILIN CAO, M.D.. Ph.D.
TISSUE ENGINEERING Volume 9, Suppl. 1, 2003,-0001,():
-1年11月30日
Tissue engineering started in late 1980s and is now well established and progressing rapidly in Western developed countries. However, the development of tissue-engineering research in China remains relatively unknown to the international society of tissue engineering. Although involved in all areas of tissue-engineering research, including the creation of new scaffold materials, in vitro studies of seed cells, application of growth factors, and modification of seed cells and scaffold materials, China has put special emphasis on tissue construction in large mammalian animals in order to establish a solid scientific basis for clinical application of engineered tissues. To provide a closer view of tissueengineering research in China. this article reviews our experience in tissue construction and tissue repair using immunocompetent animals such as sheep, pig, and dog as well as hen and rabbit. The engineered tissues include bone, cartilage, tendon, skin, blood vessels, cornea, and peripheral nerves.
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【期刊论文】Application of Stem Cells in Tissue Engineering
曹谊林, Cao Yilin*, Liu Wei
Stem Cell and Cellular Therapy. Vol. 1, No.1, Mar. 2003,-0001,():
-1年11月30日
Tissue engineering Bone marrow stromal cell Embryonic stem cells Manus, c, r, i, p, t, information
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曹谊林, LIU Wei, CHUA Chek-Hau, SHANG Qing-xin, QIAN Yun-liang, CAO Yi-lin
Journal of Shanghai Second Medical University 2003 Vol. 15 No.1,-0001,():
-1年11月30日
Objective To determine over-expression of a truncated type Ⅱ TGF-β receptor in doum-regulating TGF-β1 autoproduction in normal dermal fibroblasts. Methods In vitro cultured dermal fibro-blasts were treated with rhTGF-β1 (5ng/ml) or recombinant adenovirus containing a truncated type Ⅱ TGF-β receptor gene (50 pfu/cell). Their effects on regulating gene expression TGF-β1 were observed with. Northern Blot. Results rhTGF-β1 up-regulated the gene expression of TGF-β1 (34%~150%) and type I procollagen (13%~190%). Over-expression of a truncated receptor Ⅱ decreased the gene expression of TGF-β1 (53%~66%). Conclusion Over-expression of the truncated TGF-β receptor Ⅱ down-regulated TGF-β1 auto production via blocking signal transduction of TGF-β. This study may provide a new strategy for scar gene therapy.
TGF-β1 autoproduction signal transduction gene therapy
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【期刊论文】Bridging Tendon Defects Using Autologous Tenocyte Engineered Tendon in a Hen Model
曹谊林, Yilin Cao, M.D., Ph.D., Yongtao Liu, Wei Liu, Qingxin Shan, Samuel D. Buonocore, B.S., and Lei Cui, Ph.D
,-0001,():
-1年11月30日
Tendon detects remaln a realol concern in plastic surgery because of the limlted availalailicv of tertdon autografts. Whereas immune rejection prohibits the use of tendon allografts, most prosthetic replacements also fail to achieve a sausfactory long-term result of tendon repair. The tissue engineering technique however.can generare differet tissues using autologous cells and thus provide an optimal approach to address this concern. The Purpose of this study was to test the feasibility of engineering tendon tissues with autologous tenocytes to bridge a tendon defect in either atendon sheath open model or a partial open model in the hen. In a total of 40 Leghorn hens, flexor tendons were harvested from the left feet and were digested with 0.25% type II collagenase. The isolated tenocytes were expanded in vitro and mixed with unwoven polyglycolic acid fibers to form a cell-caffold construct in the shape of a tendon. The constructs were wrapped with intestinal submucosa and then cultured in Dulbeecco's Modified Eagle Medium plus 10% fetal bovine serum for 1 week before in vivo transplantation. On the feet. a defect of 3 to 4cm was created at tile second flexor digitorum profundus tendon by resetting a tendon fragment. The defects were bridged either with a cell-scaffold, construce in the experlmental group (n=20) or with scaffold material alone in the control group (n=20). Specimens were harvested at 8, 12, and 14 weeks postrepair for gross and histologic examinaltion and for biomechanical analysis. In the experimental group, a cordlike tissue hridging the rendon defect was formed at 8 weeks postrepair. At 14 weeks, the engineered tendons resembled the natural tendons grossly in both color and texture. Histologic examination at 8 weeks showed that the neo-tendon contained abundant tenocytes and collagen; most collagen bundles were randomly arranged. The undegraded polyglycolic acid tibers surrounded by inflammatory cells were also observed At 12 weeks, tenocytes and collagen fibers became longitudinally aligned, with good interface healing to normal tendon. At 14 weeks, the engineered tendons di■ played a typical tendon structure hardly distinguishab■ from that of normal tendons. Biomecfianical analysis den■ onstrated increased breaking strength of the engineere■ tendons with dine, which reached 83 percent of norm■ tendon strength at 14 weeks. In the control group, pol■ glycollc acid constructs were mostly degraded at 8 wee■ and disappeared at 14 weeks. However the breakir■ strength of the scaffold materials accounted for only ■ percent of normal tendon strength.The results of this study indicated that tendon tisst■ could be engineered in vivo to bridge a tendon dcfect. T■ engineered tendoms resembled natural tendons not on■ in gross appearance and histologic structure but also ■ biomechanical properties.
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曹谊林, YANCHUN LIU, M.D., , FUGUO CHEN, WEI LIU, Ph.D, LEI CUI, Ph.D., QINGXIN SHANG, WANGYAO XIA, JIAN WANG, YIMIN CUI, GUANGHUI YANG, DELI LIU, JUANJUAN WU, B.S., RONG XU, SAMUEL D. BUONOCORE, and YILIN CAO
TISSUE ENGINEERING Volume 8, Number 4. 2002,-0001,():
-1年11月30日
Large full-thickness defects of articular cartilage remain a major challenge to orthopedic surgeons because of unsatisfactory results of current therapy. Many methods, such as chondrectomy, drilling, cartilage scraping, arthroplasty, transplantation of chondrocytes, periosteum, perichondrium, as well as cartilage and bone, have been tried to repair articular cartilage defects. However, the results are far from satisfactory. In this study, we applied a tissue-englneering approach to the repair of articular cartilage defects of knee joints in a porcine model. Using isolated autologous chondroeytes, polygiycolic acid (PGA), and Plurouic, we have successfully in vivo-engineered hyaline carnage and repaired articular cartilage defects. The surface of the repaired defects appeared smooth at 24 weeks postrepair. Histological examination demonstrated a typical hyaline cartilage structure with ideal interface healing between the engineered cartilage and the adjacent normal cartilage and underlying cancellous hone. In addition, giycosaminoglyean (GAG) levels in the engineered cartilage reached 80% of that found in native cartilage at 24 weeks postrepair. Biomeehanical analysis at 24 weeks demonstrated that the hiomeehanical properties of the tissueengineered cartilage were improved compared with those at an earlier stage. Thus, the resuits of this study may provide insight into the clinical repair of articular cartilage defects.
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曹谊林, LIU Wei*, CAI Zebao, WANG Dabru, WU Xiaoli, CUI Lei, SHANG Qingxin, QLAN Yunliang and CAO Yilin
Chinese Journal of Traumatology (English Edition) 2002; 5 (2): 77-81,-0001,():
-1年11月30日
Objective: To study transforming growth factor-β1 (TGF-β1) autoproduetfan in keloid fibrobLasts and the regulatian effect of blocking TGF-β intracellular signaling on rhTGF-β1 autoproduction. Methods: Keloid fibroblasts ctltured in vitro were treated with either rhTGF-β1(5ng/ml) or recombinant adenovirus containing a truncated type II TGF-β receptor gener (50 pfu/cell). Their effects of regulating gene expression of TGF-β1 and its receptor Ⅰ and Ⅱ were observed with Northern blot. Results: rhTGF-β1 up-regulated the gene expression of TGF-β1 and receptor I. but not receptor Ⅱ. Overexpression of the truncated receptor II down-regulated the gene expression of TGF-β1 and its receptor Ⅰ. but not receptor Ⅱ. Conclusions: TGF-β1 autoproductiou was observed in keloid fibroblasts. Over-expression of the truncated TGF-β receptor Ⅱ deceased TGF-β1 autoproduction via blocking TGF-β receptor signaaling.
Receptor,, transforming growth factor beta, Signal transduction, Keloid
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