Series batch experiments were made to investigate the influences of pH and temperature on the ac2 tivity of acidogenus and acidogenus in glucose-degrading bacteria cultured in an UASB (up2flow anaerobic sludge blanket) reactor for glucose fermentation and hydrogen production. The bacteria exhibited different ca2 pability to recover to produce hydrogen at different initial pH and temperature. Hydrogen production, VFA production, COD removal and COD balance were measured at different pH and 20, 37℃ respectively with the same glucose and VSS in vials. Results showed that there are different influences on the activity of acido2 genic bacteria at varied pH and result in a variety of amount of hydrogen production, specific hydrogen production and VFA production, etc. Through the present study, when nonmalized to the weight of VSS, a maximal biogas and hydrogen production of 171711ml/g and 87010ml/g were obtained when pH equals 9 at 37℃ and 679100ml/g of biogas, 246135ml/g of hydrogen were also got when pH equals 5 at 20℃ respective2ly. The maximal specific hydrogen production (SHA) was 116156 ml/h, garound 8 of pH value at 37℃ and 6146ml/h, garound 4 of pH value at 20℃, which were obtained by calculating the slope of the accumulated hydrogen gas via time. Butyric acid fermentation was important for hydrogen production. Large quantity of unknown COD was found in the vials when a small quantity of bio2gas was produced, but relative less un2known COD was determined when there was large quantity of hydrogen produced. This revealed a better engi2neering foreground for application of hydrogen bio2production.