杨新林
富勒烯衍生物的合成与生物学研究;生物材料组织工程研究;灵芝抗肿瘤生物活性研究。
个性化签名
- 姓名:杨新林
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
光学
- 研究兴趣:富勒烯衍生物的合成与生物学研究;生物材料组织工程研究;灵芝抗肿瘤生物活性研究。
杨新林,博士、教授、博士生导师。1983年9月考入北京师范大学生物系,1987年获理学学士学位,1992年7月获理学博士学位,9月进入中国科学院动物研究所博士后流动站工作。1994年9月分配到北京理工大学材料科学研究中心,被聘为副研究员并担任生物材料与生物技术研究室主任。1999年7月成为北京理工大学教授。1999年起一直受聘为国家自然科学基金项目同行评议专家。2002年7月至2004年3月任北京理工大学生命科学与技术学院副院长。2002年5月任北京生物医学工程会组织工程专业委员会理事。2002年12月被授予博士生导师资格。2004年3月―2005年3月在美国MDANDERSON癌症中心做访问教授。
主要科研工作有:富勒烯衍生物的合成与生物学研究;生物材料组织工程研究;灵芝抗肿瘤生物活性研究。近年来负责或参加了包括国家重点基础研究项目973项目,国家自然科学基金项目,国家重点科技(攻关)项目,教育部高校博士基金在内的多项课题研究。至今在国内外重要刊物上发表论文100多篇,其中SCI收录19篇,EI收录19篇,ISTP收录3篇;发表国内外会议论文24篇;获得国家发明专利3项(均为第一作者),作为主要编写人员编写著作2部。
同时还坚持研究生课程教学及毕业论文指导工作。近5年培养硕士生20人,博士生10人。指导的学生作品获2003年第八届“挑战杯”全国大学生课外学术科技作品竞赛三等奖,作为副导师协助指导的博士生论文获2004年全国优秀博士论文提名奖。
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14
【期刊论文】单加成环丙烷富勒烯膦酸酯衍生物的合成与电化学性能
杨新林, YANG, Xin-Lin, QIAO, Xin-Ge, ZHU, Zhang-Guang, CHENG, Fu-Yong, He-Sun, FAN, Lou-Zhen
有机化学2006年第26卷第11期, 1557-1561/Chinese Journal of Organic Chemistry Vol. 26, 2006 No. 11, 1557-1561,-0001,():
-1年11月30日
在Mn(OAc)3•2H2O 催化下, C60分别和亚甲基二膦酸四乙酯、氰基亚甲基膦酸二乙酯或乙氧羰基亚甲基膦酸二乙酯在氯苯中回流, 生成3个单加成环丙烷富勒烯膦酸衍生物C60C(R)PO(OEt)2 [1, R=PO(OEt)2; 2, R=COOEt; 3, R=CN]. 与以前报道的Bingel反应法相比, 该方法副产物少并且缩短了反应时间. 采用循环伏安法发现1, 2的还原电位相对于C60发生负移, 而3的还原电位相对于C60却正移40 mV, 表明引入象氰基一样具有很强吸电子能力的取代基团, 可以改善富勒烯球的电化学性能, 合成电子接受能力较强的富勒烯衍生物.
单加成环丙烷富勒烯膦酸衍生物, 合成, 电化学性能, 循环伏安法
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【期刊论文】Preparation of bioelectret collagen and its influence on cell culture in vitro
杨新林, X. L. Yang, J. W. Gu, H. S. Zhu
J Mater Sci: Mater Med (2006)17: 767-771,-0001,():
-1年11月30日
Collagen extracted from pigskin was coronacharged negatively by a specifically designed device. Different charging voltages, temperatures and times were applied to prepare collagen bioelectret. The decline of the surface potential of the bioelectret under different treatment was then determined. The data showed that the surface potential was markedly varied with the charging conditions. The optimal values of three parameters for charging collagen coatings were defined as follows: voltage, 8 KV; temperature, 40◦C; time, 25 min. Treatment of the bioelectret with distillated water, or saline solution (0.9%) or culture medium induced a sharp decrease of the surface potential. In addition, we investigated the effects of the charged collagen on cell growth and intracellular calcium level of three types of cultured mammalian cell lines, including Chinese hamster ovary CHO cells, human cervix uteri tumor HeLa cells and human promyelocytic HL-60 cells. Cell growth and the intracellular calcium level were determined by MTT reagent-based assay and a fluorescent probe Fura-2, respectively. The results showed that negatively charged collagen stimulated the growth of CHO or HL-60 cell line but inhibited the growth of HeLa cell line. Furthermore, after attaching to the charged collagen, the intracellular calcium level of CHO cells increased, while that of HeLa cells decreased.
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【期刊论文】Inhibition of M-MuLV reverse transcriptase activity by fullerene derivatives
杨新林, MENG Xianmei, CHEN Zhe, LI Bo, ZHANG Yufei, ZHAO Dongxu, YANG Xinlin
Chinese Science Bulletin 2006 Vol. 51 No.20 2550-2552,-0001,():
-1年11月30日
Influence of fullerene (C60) derivatives on M-MuLV reverse transcriptase activity is investigated. Two water-soluble fullerene derivatives, fullerol (C60(OH)23-24) and trimalonic acid C60 (TMA C60, C63(COOH)6) are used in the experiments and their effects on in vitro reverse transcription-polymerase chain reaction (RT-PCR) of β-actin mRNA in HeLa cells are determined. PCR products are detected by agarose gel electrophoresis. It is found that the amounts of PCR products decrease with addition of either of two fullerene derivatives to RT reaction mixture. The inhibition of fullerene derivatives is dose-dependent, and IC50 values of fullerol and TMA C60 are 0.089 and 0.039 mmol/L, respectively. The amount of PCR products obtained by direct addition of fullerol or TMA C60 to PCR are greater than those obtained by addition of the equivalent amount of fullerol or TMA C60 to RT, indicating an inhibitory effect of fullerol or TMA C60 on RT. The compensative experiment of M-MuLV reverse transcriptase shows that increasing enzyme amounts can antagonize the activity of fullerol or TMA C60. These results imply that fullerenes can inhibit M-MuLV reverse transcriptase activity, with the inhibition of TMA C60 slightly stronger than fullerol, and that their potential in treatment of diseases induced by RNA viruses such as leukemia virus needs further investigation.
fullerene derivatives, M-MuLV reverse trans, c, r, i, p, t, ase, inhibition
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【期刊论文】Inhibition of DNA restrictive endonucleases and Taq DNA polymerase by trimalonic acid C60
杨新林, YANG XinLin, CHEN Zhe, MENG XianMei, LI Bo, TAN Xin
Chinese Science Bulletin July 2007 Vol. 52 No. 13 1802-1806,-0001,():
-1年11月30日
Activities of trimalonic acid fullerene (TMA C60) on DNA restrictive enzymatic reaction were investigated by using two restrictive endonucleases Hind III and EcoR I and plasmid pEGFP-N1 with single restrictive site for both enzymes. Meanwhile, TMA C60 was also tested to clarify its effects on polymerase chain reaction (PCR) with the catalyst of Taq DNA polymerase and the template of plasmid pEGFP-N1. The products from restrictive reactions or PCR were detected by agarose gel electrophoresis. It was found that the product amounts from restrictive reactions or PCR decreased significantly with addition of TMA C60. The inhibition by TMA C60 was dose-dependent and IC50 values for reactions of Hind III, EcoR I and PCR were 16.3, 6.0 and 6.0 μmol/L, respectively. Addition of two scavengers of reactive oxygen species (ROS), L-ascorbic acid-2-phosphate ester magnesium and sodium azide at the concentrations of 2―10 mmol/L did not antagonize the activities of TMA C60 against PCR and two restrictive reactions. However, increase of Taq DNA polymerase amounts in PCR system antagonized the activities of TMA C60. These data implied that TMA C60 was able to inhibit the activities of the three above-mentioned enzymes involved in DNA metabolism, and that this inhibition probably did not correlate to ROS.
trimalonic acid C60 (, TMA C60), , DNA restrictive endonucleases, Taq DNA polymerase, reactive oxygen species, inhibition
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杨新林, Xinlin Yang, Lin Chen, Xinge Qiao, Cuihong Fan
International Journal of Toxicology, 26: 197-201, 2007,-0001,():
-1年11月30日
The biological activities of fullerene derivatives have attracted much attention in the last decade. In this paper, effects of dimalonic acid C60 (DMA C60) on cytoplasmic membrane, intracellular calcium concentration ([Ca2+]i), and mitochondrial membrane in HeLa cells were studied by using laser scanning confocal microscopy together with fluorescent probes propidium iodide (PI), fluo-3 acetoxymethyl ester (fluo-3 AM), and tetramethyl rhodamine methyl ester (TMRM). The data showed that under laser irradiation produced by a Kr/Ar laser source with a low power less than 1 mW, DMA C60 might induce damages against both cytoplasmic and mitochondrial membranes in a time- and dose-dependent manner. Prior to leakage of cytoplasmic membrane, a transient increase in [Ca2+]i occurred due to influx of calcium from the culture medium. These data provided some novel clues to explain the mechanisms involved in the photo-induced cytotoxicity of fullerene derivatives.
Calcium Signal, Cytoplasmic Membrane, Dimalonic Acid C60, Laser Scanning Confocal Microscopy, Mitochondrial Membrane, Photo-Induced Damage
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杨新林, X.L. Yang , C. Huang, X.G. Qiao, L. Yao, D.X. Zhao, X. Tan
Toxicology in Vitro XXX(2007)XXX-XXX,-0001,():
-1年11月30日
Using human erythrocyte membranes (EMs) as a model system, we have examined photo-induced lipid peroxidation by a bis-methanophosphonate fullerene (BMPF) and four other fullerene derivatives including a mono-methanophosphonic acid fullerene (MMPF), a dimalonic acid C60 (DMA C60), a trimalonic acid C60 (TMA C60) and a polyhydroxylated fullerene (fullerol). Lipid peroxidation was assessed as the malondialdehyde (MDA) level measured by the thiobarbituric acid assay. It was observed that BMPF increased the MDA level of EMs after irradiation in both time- and dose-dependent manners. The photo-induced activity became very significant (p < 0.01) under the conditions of either the concentration of 10 μM and irradiation time of 30 min or the concentration of μ5 M and irradiation time of 60 min. Involvement of reactive oxygen species (ROS) in the activity was also examined by specific inhibitors of singlet oxygen, superoxide anions and hydroxyl radicals, respectively. While all three kinds were found responsible for the activity, the former two might play more important roles than the last one. Furthermore, the activity of BMPF was the strongest among all tested fullerene derivatives. These results indicated BMPF was a potential photosensitizer that would find application in photodynamic therapy.
Bis-methanophosphonate fullerene, Human erythrocyte membranes, Irradiation, Lipid peroxidation, Reactive oxygen species
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【期刊论文】Inhibition of a thermophilic deoxyribonucleic acid polymerase by fullerene derivatives
杨新林, XIANMEI MENG, BO LI, ZHE CHEN, LU YAO, DONGXU ZHAO, XINLIN YANG, MIN HE, QUN YU
fournal of Enzyme Inhibition and Medicinal Chemistry, June 2007; 22(3): 293-296,-0001,():
-1年11月30日
Enzyme inhibition by fullerene derivatives has attracted much attention. In this communication, effects of two water-solube fullerene derivatives, fullerol and trimalonic acid C60 (TMA C60) on polymerase chain reaction (PCR) were investigated by using PCR of β-actin cDNA derived from HeLa cells as an experimental model. Both fullerol and TMA C60 were found to inhibit PCR in a dose-dependent manner. PCR was ultimately inhibited while the concentrations of each compound were not less than 0.01mM. In contrast, mannitol exerted no effects on PCR while its concentration increased up to 2mM. Compensation experiments with Thermus aquaticus (Taq) DNA polymerase revealed that both fullerol andTMAC60 inhibited the enzymatic activity of Taq DNA polymerase, and the inhibitory potency of TMA C60 was slightly greater than that of fullerol. Our data provides some novel aspects on the enzyme inhibiting activities of fullerene derivatives.
Fullerol, trimalonic acid C60, Taq DNA polymerase, inhibition
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杨新林, H.-S. Zhu, X.-L. Yang, L.-B.Wang, D.-X. Zhao and L. Chen
Cell Biology and Toxicology. 2000; 16: 201-206.,-0001,():
-1年11月30日
The e
calcium, cell cycle, cell growth, Ganoderma lucidum, spores
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杨新林, X. L. Yang*, C. H. Fan, H. S. Zhu
Toxicology in Vitro 16 (2002) 41-46,-0001,():
-1年11月30日
Photo-induced cytotoxicity of malonic acid [C60] fullerene derivatives and its mechanism X. L. Yang*, C. H. Fan, H. S. Zhu Research Center of Materials Science, Beijing Institute of Technology, PO Box 327, Beijing 100081, PR China Accepted 10 August 2001 The biological activities of fullerenes have attracted extensive attention in recent years. The aim of this paper is to study the relation of the photo-induced cytotoxicity of fullerene derivatives to their chemical structures as well as the possible cellular mechanism involved in the photocytotoxicity. Three C60 derivatives with two to four malonic acid groups (DMA C60, TMA C60 and QMA C60) were prepared and the cytotoxicity of these compounds against HeLa cells was determined by MTT. Cell cycle was measured by flow cytometry. The results showed that the cytotoxicity of the malonic acid C60 derivatives was irradiation-and dosedependent. The sequence of their photo-induced growth inhibition was DMA C60>TMA C60>QMA C60. Hydroxyl radical quencher mannitol (10mm) was not able to prevent cells from the damage induced by irradiated DMA C60. DMA C60,together with irradiation,was found to have an ability of inducing a decrease in the number of G1 cells from 63 to 42% and a rise in that of G2+M cells from 6 to 26%. These data indicated that the number of malonic acid molecules added to C60 played an important role in the phototoxicity and the blockage of cell cycle might be a mechanism of this activity.
Cell cycle, Cell growth, Malonic acid C60 derivatives, Photo-induced, Reactive oxygen species
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杨新林, Hongbo HU, Nam-Shik AHN, Xinlin YANG, Yong-Soon LEE and Kyung-Sun KANG*
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-1年11月30日
Although the pharmacology and clinical application of water extracts of Ganoderma lucidum have been extensively documented, little is known regarding its alcohol extract. In the present study, the anti-tumor effect of an alcohol extract of Ganoderma lucidumwas investigated using MCF-7 cells. We found that the alcohol extract of Ganoderma lucidum inhibited cell proliferation in a dose-and time-dependent manner, which might be mediated through up-regulation of p21/Waf1 and down-regulation of cyclin D1. Furthermore, this compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and not by the immune system. Our findings suggest that there are multiple mechanisms underlying the anti-tumor effects of Ganoderma lucidum.
ganoderma lucidum, breast cancer, cell cycle, apoptosis
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