本实验采用无水乙醇灌胃粘膜损伤后，分别电针足阳明经“四白”、“梁门”、“足三里”三个不同段代表穴，及“足三里”外2cm的对照点，观察其对胃粘膜损伤后的细胞保护作用，以证实足阳明经与胃的相关性。结果发现，分别电针“四白”、“梁门”、“足三里”7日后，均能使胃粘膜损伤指数显著降低。电针“足三里”后，胃液及胃粘膜PGE2与其他各组比较均显著增高（p<0.05或0.01），血清NO与模型组、电针“足三里”外2cm组比较亦见明显增高（p<0.05）；电针“四白”、“梁门”、“足三里|、以及空白组胃粘膜EGF与未经电针的模型组比较均有显著差异（p<0.05或0.01）。提示，电针家兔足阳明经不同节段的腧穴均对胃粘膜损伤细胞有保护作用，其中以”足三里“最满意，说明足阳明经与胃具有相关性，与此同时，同一经脉的穴位对相关脏腑的作用亦有着相对的特异性。

目的：观察针刺后血清对胃溃疡大鼠胃黏膜细胞c-myc 基因表达的影响。方法：60 只大鼠随机分为5 组，利用链霉蛋白酶消化法分离胃黏膜细胞，分别用表皮生因子受体（EGFR）抑制剂PD153035 和血清孵育胃黏膜细胞，采用逆转录聚合酶链反应法检测胃黏膜细胞c-myc 基因的表达水平。结果:正常血清组大鼠胃黏膜细胞c-myc mRNA 有微弱表达；胃经血清组和对照血清组大鼠胃黏膜细胞c-myc mRNA 呈现较强上增性表达,其中胃经血清组大鼠上增性表达更加明显，两组相比较有显著性差异(P<0.01)；胃经血清+抑制组和对照血清+抑制组大鼠胃黏膜细胞c-myc mRNA 可见少量表达，分别与胃经血清组和对照点血清组两两相比较有显著性差异 (P<0.01)。结论：针刺血清能刺激胃黏膜损伤修复信号转导通路中c-myc 基因的活化，进而诱导细胞增生，并且存在经脉-脏腑的特异性联系。

Acupuncture has been widely applied to treat gastrointestinal diseases in the clinic. However, its precise mechanism remains unknown. In the present study, effects of electroacupuncture (EA) at Zusanli acupoints (ST36) on gastric myoelectric activity and substance P (SP) in the dorsal vagal complex (DVC) were observed. EA at ST36 significantly increased the frequency of slow waves and the frequency of the spike potentials superimposed on the slow waves, which persisted for 90 min after cessation of EA and was completely abolished after bilateral vagotomy. Meanwhile, the mean optical density of SP immunoreactive fibers in the DVC increased following EA. The results suggested that EA at ST36 promoted the gastric myoelectric activity, which was regulated by the vagus, and SP in the DVC may be involved in the excitatory effects. Furthermore, EA had a relative long-term post-effect. We presumed that DVC may be a supraspinal center in the effect of EA on gastric function. q 2003 Elsevier Ireland Ltd. All rights reserved.

Clinical practice has shown that acupuncture at facial acupoints has curative effects onsome visceral diseases (especially gastrointestinal diseases). However, the physiological basis has not been clarified yet. In the present study, expression of c-fos in the nucleus of the solitary tract (NTS) of rats following gastric distension and electroacupuncture (EA) at Yangbai (GB14) and Sibai (ST2) as well as Jiache (ST6) acupoints was observed by using immunohistochemistry technique. After EA at the three facial acupoints, c-fos immunoreactive (c-fos-IR) neurons were mainly distributed in the medial (mNTS) and intermediate subnucleus of the NTS, and a few were scatteredly distributed in the dorsalmedial and commissural subnucleus of the NTS. Furthermore, there is difference in the number of c-fos-IR neurons in the mNTS following EA at the three facial acupoints. The number in the EA at ST2 and GB14 group is the highest and the lowest, respectively. Gastric distension induces obviously the expression of c-fos, which is mainly confined in the mNTS. The results suggest that the noxious visceral and somatic afferent information from the stomach and face may converge in the mNTS, which may be involved in the effect of EA at facial acupoints on the gastrointestinal pain.