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2005年03月08日

【期刊论文】超高产杂交稻灌浆期光合功能与产量的关系*

万建民, 翟虎渠**, 曹树青, 陆巍, 张荣铣**李良璧, 匡廷云, 闵绍楷, 朱德峰, 程式华

中国科学(C辑)2002,32(3):211~217,-0001,():

-1年11月30日

摘要

以杂交水稻协优63为对照,对亩产800kg以上的超高产杂交水稻组合协优9308抽穗前、后的物质生产特性及剑叶光合作用与穗部物质积累的关系进行研究。结果表明:(i)与协优63相比,协优9308抽穗前、后的物质生产能力无论生物量还是日干物质生产量,均极显著高于协优63,尤以抽穗后更为明显。(ii)协优9308剑叶的光合碳同化能力(叶源量)极显著高于协优63,且能够切合籽粒灌浆需求,在单株水平上其净同化产物基本满足籽粒灌浆的需求,而协优63抽穗后20d左右剑叶光合功能快速衰退,单株净同化产物不能满足籽粒灌浆的需求。结果证明,灌浆后期仍能保持高效光合功能、并且能够切合籽粒灌浆需求是实现水稻超高产的关键环节。

水稻,, 超高产,, 光合作用,, 籽粒灌浆

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2005年03月08日

【期刊论文】利用回交重组自交群体检测水稻抗褐飞虱数量性状基因座

万建民, 苏昌潮, 程遐年, 翟虎渠, 万建民①

遗传学报,29 (4): 332~338, 2002,-0001,():

-1年11月30日

摘要

利用由98个家系组成的Nipponbare/Kasalath∥Nipponbare回交重组自交系(Backcross Inbred lines,BILs)作图群体(BC1F9),进行水稻抗褐飞虱数量性状基因座(Quantative Trait Locus, QTL)的检测和遗传效应分析。采用苗期集团鉴定方法,并以死苗率作为抗褐飞虱表型值,分析亲本和98个BILs的抗褐飞虱表现。共检测到3个苗期抗褐飞虱QTL,分别位于2、10和12染色体上。各QTL的LOD值为2101~2133,贡献率为1014%~1616%,可解释群体总表型变异的3910%。这3个数量性状基因座对褐飞虱的抗性均来自抗虫亲本Kasalath。与这些数量性状基因座连锁的分子标记可望应用于聚合多个抗性基因,培育对褐飞虱具有水平抗性水稻新品种的育种实践中。

水稻, 抗褐飞虱, 回交重组自交(, BIL), 群体, 数量性状基因座(, QTL), 分析

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2005年03月08日

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2005年03月08日

【期刊论文】Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.)

万建民, J. Wan. Y. Yamaguehi

Theor Appl Genet (1996) 92: 183~190,-0001,():

-1年11月30日

摘要

Female gamete abortion in Indica-Japonica crosses of rice was earlier identified to be due to an allelic interaction at the S-5 locus on chromosome 6. Recently. in other crosses of rice, similar afielic interactions were found at loci designated as S-7 and S-8, located on chromosomes 7 and 6 respectively. All of them are independent of each other. At the S-5 locus, Indica and Japonica rice have S-5i and S-Y alleles respectively and Javanieas, such as Ketan Nangka, have a neutral allele S-5". The S-5i/S-5j genotype is senti-sterile due to partial abortion of female gametes carrying S-U, hul both the S 5"/S-5i and S-5"/S-5J genotypes are fertile. The S-5" allele is thus a "wide-compatibility gene" (WCG), and parents homozygous for this allele are called wide-com patible varieties (WCV), Such parents when crossed with Indica or Japonica varieties do not show F1 hybrid sterility. Wide compatible parents have been used to overcome sterility barriers in crosses between Indica and Japonica rice, However, a Javanica variety, Ketan Nangka (WCV), showed typical hybrid sterility when crossed to the Indian varieties N22 and Jaya. Further. Dular+ another WCV from India, showed typical hybrid sterility when crossed to an I RRI line, IR2061-628 1-6-4-3(IR2061-628). By genetic analyses using isozyme markers, a new locus causing hybrid stetihty in crosses between Ketan Nangka and the Indicas was located near isozyme loci Est-I and Mal-I on chromosome 4, and w as designated as S-9. An other new locus for hybrid sterility in the crosses between Du]ar and the IR2061 628 was identified and was found lin ked to four isozyme loci, Sdh-l, Pox-g, Aep-1 and Acp-2. on chromosome 12 It was designated as S-15. On the basis of allelic interac tlons causing female-gamete abortion, two alleles were found at S-9, S-9kn in Ketan Nangka and S-9iin N22 and Jaya. In the heterozygote, S-9kn/S-9i, which was semisterile, female gametes carrying S-9kn" were aborted. The hybrid of Dular and IR2061-628, with a genetic constitution of S-15Du/S-I5i, was semi-sterile and the female gametes carrying S-I 5Du were aborted. A Japonica tester variety, Akihikari, and an Indica variety, IR36, were found to have neutral alleles, S-9n and S-15n, at these loci, in addition to S-7" and at S-7. The accumulation of three neutral alleles into a breeding line should help solve the hybrid sterility problem in wide crosses office.

Oryza sativa

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2005年03月08日

【期刊论文】Identification of Marker Loci for Seed Dormancy in Rice (Oryza saliva L.)

万建民, I Wan, T. Nakazaki, * K. Kawaura, and H. Ikehashi

Reprinted from Crop Science Vol. 37. No.6,-0001,():

-1年11月30日

摘要

In rice breeding, genes for seed dormanoy as well as the markers for each of them are required of an incorporation of an adequate seed domancy into japonica cultivrs To screen genetic Ioci for seed dormancy in rice (Orvza saliva L.), two kinds of populations were tested: (i)an Fz population of a hybrid Milyang 23 I Todorokiwase. and (fi) Fl populations from a three-way cross, in which an indica donor of strong dormancy, IR 36, was crossed with non-dormant japonica cultivars, Nekken 2 and Miyukimochi, in the form of IR 36/ Nekken 2// Miyukimochi. The seeds predated on the F2 plants from the single cross and those on FI plants from the three-way cross were tested at three times: immediately after maturation, after 60 d storage at 4C, and after breaking dormancy (7 d at 50C). Of 24 marker Ioci assayed, at least fore loci linked to seed dormancy were detected. The strong seed dormancy of Milyang 23 was linked with three isozyme genes: Pgi-I1 on chrompsome 3, Amp-32 on chromosome 6, and Est92 on chrmosome 7, while the strong seed dormancy of IR 36 was linked with C+ (apiculus color) on chromosome 6, Est-92 on Chromosome 7, and Acp-21 an chromosome 12. The high level of seed dorloci. Differential responses of inch Iocl to a dormacy-breaking treatment were detected. An allele from IR 36 at a locus on chromosome 6 seemed to be responsible for strong seed dormany after maturation and to be easliy broken during storage. This allele may be incorporated into japonica ries by means of marker C for apiculus color.

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    南京农业大学,江苏

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