目的研究DPC4（deleted in pancreatic cancer locus 4）基因在胰腺癌中的改变。方法用多聚酶链反应2单链构象多态性分析（PCR2SSCP）银染技术及PCR产物直接测序法检测胰腺癌细胞系P1、P2、P3、P4、P7，11例新鲜冷冻胰腺癌组织中DPC4基因第1，2，3，4，8，11外显子的缺失和突变。结果5株人胰腺癌细胞系中，3株（P1、P2、P3）存在DPC4基因突变,DPC4 基因改变率为3/5。11例新鲜冷冻胰腺癌组织中，3例有DPC4基因的纯合性缺失，2例有基因突变，DPC4基因改变率为5/11(45.5%)。结论DPC4作为一种抑癌基因，其改变在胰腺癌的形成中可能起重要作用。

We report here the two-dimensional protein database of human pancreas. The proteins were analyzed by two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Totally, 302 proteins were identified, of which about 27% were enzymes with a broad range of catalytic activities. Several of these are specifically expressed in pancreas, such as pancreatic amylase, pancreatic stone protein, pancreatitis-associated protein, pancreatic lipase, pancreatic elastase, etc. Structural and cytoskeletal proteins are also strongly represented on the gels. Thus, the pancreatic proteome reflects the organ's function. This work paves the way for further studies on pancreatic protein expression in health and disease, such as diabetes and pancreatic cancer.