已为您找到该学者10条结果 成果回收站
徐群渊, Q. Xu, and G. Grant
THE JOURNAL OF COMPARATIVE NEUROLOGY 345:288-302(1994),-0001,():
-1年11月30日
The fiber couse of the spinocerebellar tracts in the ventral and lateral funiculi of the cat spinla cord were studied by a new approach making cordotomies at different spinal levels or lesions of the restiform body followed by injectionas of HRP or WGA-HRP into the anterior cerbellar olbe. The retrogradely labeled axons showed characteristic distribution patterns ralated to the level and extent of the lesions. The results show the following. 1) The dorsal spinocerbellar tract (DSCT) originationg ipsilaterally form the thoracic and upper lumbar segments ascends in the dorsolateral fasciculus. It underoes a dorsal shift during its rostral coures. The tract is topially arranged and passes through the restiform body. 2) The ventral spinocerebellar tract (VSCT) arising contralaterally from lower thoractic, lumbar, and more eaudal segments passes via the ventral funiculus and ascends in the ventrolateral fasciculus. This tract is also topically arranged. It maks a lateral and then a dorsal region enetrs via the restiform body. 3) The spnocerebellar fibers oriinationg ipsilaterally from the cervical enlafrgement ascend in the lateralmost part of the lateral funiculus in the area between the dorsolateral and ventrolateral fasciculi, There fibers form two groups, one passing through the dorsolateral and ventrolateral fasciculi. These form two groups, one passing through the restiform body, the other through the superior cerbellar peduncle. 4) The spinocerebellar fibers origination comtralaterally form the central cervical nucaleus pass through the ventral funiculus and ascend in the lateralmost part of the lateral funiculus, mainly inthe ventrolateral fasciculus. Most of the fibers seem to pass through the superior cerebellar peduncle.
ascending pathways,, cerebellum,, HRP,, WGA-HRP,, retrograde transport
-
136浏览
-
0点赞
-
0收藏
-
0分享
-
47下载
-
0
-
引用
【期刊论文】An experimental study on rat model of parkinsonizm by gene therapy
徐群渊, Xu Qunyuan, Tian Jingsheng, Zhang Jinlu, Yang Hui, Zheng Shaopeng, and Liu Yujun
Chinese Medical Journal 1998; 111(2):154-159,-0001,():
-1年11月30日
Objective To induce significant improvement of motor abnormalitie s and striatal dopamine (DA) levels in rat model of Parkinson's disea se (PD), by intrac2 erebral grafting of the genetically modified muscle cells expressing tyro sine hydroxyla se (TH). Me t h o ds Primary myobla st s and myotube s from the rat were prepared by cell culture and a plasmid, pCMVTH, containing TH gene and a promoter of cytomegalovirus (CMV) wa s constructed by DNA recombination technique. The primary muscle cells were transfected with newly constructed pCMVTH DNA vector, by using lipofection. The se genetically modified muscle cells were grafted into the caudate-putamen of 62OHDA2le sionedrats, repre senting PD models. Before and after grafting, the rotational behaviour and the striatal levels of DA and it s metabolitie s were te sted at different po stoperative survival time s. In addition, the immunocy to chemistry for showing TH activity wa s done both in vitro and in vivo. Results The newly constrcuted pla smid, pCMVTH wa s proved to contain TH gene and have correct direction of insertion. The cultured primary myobla st s and myotube s lipofected with pCMVTH were immunocytochemically shown to expre ss TH activity in vitro. After grafting, the se TH2expre ssing muscle cells showed to have a long2term survival cells in vivo and induced a marked decrea se in abnormal locomotion and a increa se in striatal DA levels for PD rat model. Conclusions In experimental gene therapy for PD, the pCMVTH is a useful vector for carrying TH gene. The lipofection is a practical technique for transferring a target gene into eukaryote s and primary cultured muscle cells should be a good vehicle for DNA transfer and intracerebral grafting.
-
98浏览
-
0点赞
-
0收藏
-
0分享
-
127下载
-
0
-
引用
【期刊论文】TH和GDNF基因工程细胞脑内移植治疗PD大鼠的实验研究
徐群渊, 张京钟, 杨慧, 段德义, 段春礼, 孙晓红, 赵春礼, 张进禄, 徐群渊*
解剖学报,2000,31:32~36,-0001,():
-1年11月30日
目的 观察酪氨酸羟化酶(TH)和胶质细胞系源性神经营养因子(GDNF)基因工程细胞联合移植到纹状体内对帕金森病(PD)大鼠的治疗作用。方法 重组携带TH基因的质粒表达载体pCMVTH及携带GDNF基因的质粒表达载体DCl。neo,分别转染原代培养的成纤维细胞。筛选阳性细胞植入大鼠帕金森病模型纹状体内,动态观察行为学变化,并用高效液相色谱于第4、8、12、16、20周测定多巴胺、3。4-二羟苯乙酸(DOPAc)及高香草酸(HVA)含量的变化。结果 联合移植TH及GDNF转基因细胞致PD大鼠纹状体内可明显减少模型单侧旋转行为,可降至移植前旋转圈数的44.3±5.2%(P<0.01),其效果优于单独移植TH(65.6±6.1%)或GDNF(64.8±5.8%)转基因细胞的PD模型(P<0.05);显著提高纹状体内多巴胺含量。恢复至移植前水平的21.4±3.47%(P<0.001),其程度高于单独移植TH(12.9=1.4%)或GDNF(14.5±2.2%)转基因细胞的PD模型(P<0.05)。结论 TH及GDNF联合基因治疗具有潜在的临床应用价值。
帕金森病, 基因治疗, 酪氨酸羟化酶(, TH), 胶质细胞系源性神经营养因子(, GDNF)
-
98浏览
-
0点赞
-
0收藏
-
0分享
-
109下载
-
0
-
引用
徐群渊, 陈立南, 徐群渊*
神经解剖学杂志,2000,16(3):217~221,-0001,():
-1年11月30日
用计算机三维重构技术建立了人及大鼠基底神经节的三维数字化模型,并对两者的形态及核团构成进行了比较解剖学研究。在微型计算机上,提取人脑及大鼠脑立体定位图谱中含有基底神经节的连续层面,利用通用的三维建模及动画软件3DStudio MAX,以三维放样的方法,在我国首次建立人及大鼠基底神经节的核团及纤维传导通路的三维数字模型,对人及大鼠的基底神经节进行了比较解剖学研究。并利用重构的三维模型建立虚拟现实文件,在神经解剖学研究中引入浏览器虚拟现实技术,实现了任意拆分、组装及旋转上述核团的功能。本研究显示,无论在形态上还是在毗邻关系上,人与大鼠的基底神经节的差异不大;由于人的直立而导致脑干吻尾轴旋转了一定角度,其内部核团的相互毗邻关系也随之发生变化,从而异于大鼠基底神经节内部核团的毗邻关系;基底神经节各个核团间的投射纤维以尽可能短的路径到达靶核团的相应区域,构成了拓扑投射关系的基础。
三维重构, 基底神经节, 比较解剖学, 虚拟现实, 人, 大鼠
-
84浏览
-
0点赞
-
0收藏
-
0分享
-
248下载
-
0
-
引用
徐群渊, S. Yu, J.Z. Zhang, C.L. Zhao, H.Y. Zhang & Q. Xu∗
Biotechnology Letters 26:1131-1136, 2004.,-0001,():
-1年11月30日
A fast and effective method to enrich large number of neural precursors from the ventricular zone of human fetus by magnetic affinity cell sorting (MACS) is reported. After incubation with phycoerythrin (PE)-conjugated anti CD133 antibodies and anti-PE magnetic beads followed by one cycle of MACS, CD133+ cells were harvested at 85% purity as confirmed by flow-cytometry and immunocytochemistry. In contrast to CD133-cells, these CD133+cells initiated primary and secondary neurospheres in culture, and the progeny of sorted cells could be differentiated into both neurons and glia, indicating that these highly enriched cells are capable of self-renewal and multi-lineage potential.
D133,, magnetic affinity cell sorting,, neural precursors,, stem cells
-
81浏览
-
0点赞
-
0收藏
-
0分享
-
100下载
-
0
-
引用