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【期刊论文】The Permeability and Cytotoxicity of Insulin-Mimetic Vanadium Compounds
杨晓达, Xiao-Gai Yang, Xiao-Da Yang, , Lan Yuan, Kui Wang, and Debbie C. Crans
Pharmaceutical Research, Vol. 21, No.6, June 2004,-0001,():
-1年11月30日
Purpose. The aim of this study was to investigate the mechanism of permeation and cytotoxicity of vanadium compounds, [VO(acac)2], [VO(ma)2], and vanadate. Methods. Absorptive transport were carried out in Caco-2 monolayers grown on transwell inserts. Vanadium was quantified using inductively coupled plasma atomic emission spectrometry (ICP-AES). The change of Caco-2 cells in the microvilli morphology and F-actin structure was visualized by transmission electron microscopy and confocal laser scanning microscopy. Results. The three vanadium compounds were taken up by Caco-2 cells via simple passive diffusion. [VO(acac)2] were mainly transcellularly transported and exhibited the highest apparent permeabilty coefficients (8.2 × 10−6 cm-1). The cell accumulation of [VO(acac)2] was found to be greater than that of [VO(ma)2], and vanadate caused much less accumulation than the other two compounds. Vanadium compounds induced intracellular reactive oxygen species, reduced the transepithelial electric resistance, caused morphological change in microvilli, and led to different perturbation of F-actin structure. Conclusions. The three compounds exhibited different permeability due to different diffusion process and cellular uptake. The toxicity of vanadium complexes on Caco-2 monolayer involved F-actin-related change of tight junction and impairment of microvilli. The toxicity was also related to elevated intracellular reactive oxygen species (ROS) and their cellular accumulation.
Caco-2 cells, confocal laser scanning microscopy, cytotoxicity, F-actin, vanadium compounds.,
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杨晓达, Xiaoda Yang, ‡ Dan Yin, ‡ Stanislaw F. Wnuk, § Morris J. Robins, | and Ronald T. Borchardt*, ‡
Biochemistry 2000, 39, 15234-15241,-0001,():
-1年11月30日
In an effort to design more specific and potent inhibitors of S-adenosylhomocysteine (AdoHcy) hydrolase, we investigated the mechanisms by which 5',5',6',6'-tetradehydro-6'-deoxy-6'-halohomoadenosines (X=C1, Br, I) inactivated this enzyme. The 6'-chloro (a) and 6'-bromo (b) acetylenic nucleoside analogues produced partial (50%) loss of enzyme activity with a concomitant (50%) reduction of E-NAD+ to E-NADH. In addition, Ade and halide ions were released from the inhibitors in amounts suggestive of a process involving enzyme catalysis. AdoHcy hydrolase, which was inactivated with compound a, was shown to contain 2 mol of the inhibitor covalently bound to Lys318 of two subunits of the homotetramer. These data suggest that the enzyme-mediated water addition at the 5'position of compound a or b produces an R-halomethyl ketone intermediate, which is then attacked by a proximal nucleophile (i.e., Lys318) to form the enzyme-inhibitor covalent adduct (lethal event); in a parallel pathway (nonlethal event), addition of water at the 6'position produces an acyl halide, which is released into solution and chemically degrades into Ade, halide ion, and sugar-derived products. In contrast, compound c completely inactivated AdoHcy hydrolase by converting 2 equiv of E-NAD+ to E-NADH and causing the release of 2 equiv of E-NAD+ into solution. Four moles of the inhibitor was shown to be tightly bound to the tetrameric enzyme. These data suggest that compound cinactivates AdoHcy hydrolase by a mechanism similar to the acetylenic analogue of Ado described previously by Parry et al. [(1991) Biochemistry 30, 9988-9997].
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【期刊论文】Binding of Cu2+ to S-adenosyl-L-homocysteine hydrolase
杨晓达, Yanjie Li a, Jiejin Chen a, Jing Liu a, Xiaoda Yang a, b, *, Kui Wang a
Journal of Inorganic Biochemistry 98(2004)977-983,-0001,():
-1年11月30日
S-Adenosylhomocysteine (AdoHcy) hydrolase regulates biomethylation and homocysteine metabolism. It has been proposed to be a copper binding protein playing an important role in copper transport and distribution. In the present work, the kinetics of binding and releasing of copper ions was studied using fluorescence method. The dissociation constant for copper ions with AdoHcy hydrolase was determined by fluorescence quenching titration and activity titration methods using ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), and glycine as competitive chelators. The experimental results showed that copper ions bind to AdoHcy hydrolase with a Kd of~10-11M. The association rate constant was determined to be 7×10-6 M-1 s-1. The releasing of copper ions from the enzyme was found to be biphasic with a k(1) of 2.8×10-3 s-1 and k(2) of 1.7×10-5 s-1. It is suggested that copper ions do not bind to the substrate binding sites because the addition of adenine substrate did not compete with the binding of copper to AdoHcy hydrolase. Interestingly, it was observed that EDTA could bind to AdoHcy hydrolase with a dissociation constant of K1=8.0×10-5M and result in an increased affinity (Kd=~10-17M) of binding of copper ions to the enzyme.
Copper, S-Adenosyl-L-homocysteine hydrolase, Kinetic
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杨晓达, Xiaoda Yang and Ronald T. Borchardt
Archives of Biochemistry and Biophysics Vol. 383, No.2, November 15, pp. 272-280, 2000,-0001,():
-1年11月30日
The gene encoding S-adenosylhomocysteine (AdoHcy) hydrolase in Leishmania donovani was subcloned into an expression vector (pPROK-1) and expressed in Escherichia coli. Recombinant L. donovani AdoHcy hydrolase was then purified from cellfree extracts of E. coli using three chromatographic steps (DEAE-cellulose chromatofocusing, Sephacryl S-300 gel filtration, and Q-Sepharose ion exchange). The purified recombinant L. donovani enzyme exists as a tetramer with a molecular weight of~48 kDa for each subunit. Unlike recombinant human AdoHcy hydrolase, the catalytic activity of the recombinant L. donovani enzyme was shown to be dependent on the concentration of NAD1 in the incubation medium. The dissociation constant (Kd) for NAD1 with the L. donovani enzyme was estimated to be 2.1±0.2μM. The Km values for the natural substrates of theenzyme, AdoHcy, Ado, and Hcy, were determined tobe 21±3, 8±2, and 82±5μM, respectively. Several nucleosides and carbocyclic nucleosides were tested for their inhibitory effects on this parasitic enzyme, and the results suggested that L. donovani AdoHcy hydrolase has structural requirements for binding inhibitors different than those of the human enzyme. Thus, it may be possible to eventually exploit these differences to design speci®c inhibitors of this parasitic enzyme as potential antiparasitic agents.
S-adenosylhomocysteine hydrolase, Leishmania donovani, parasite, enzyme inhibitor.,
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