目的 观察培养过程中日本血吸虫成虫细胞培养液中氨基酸（AA）、葡萄糖（Gluc）及甘油三酯（TG）含量的动态变化。方法 用氨基酸自动分析仪、自动生化分析仪分别测定培养液在培养0～6d过程中AA、Gluc 及TG含量变化。结果 精氨酸（Arg）、苏氨酸（Thr）、蛋氨酸（Met）、赖氨酸（Lys）及Gluc含量有不同程度下降；天门冬氨酸（Asp）、丙氨酸（Ala）及游离氨（Amm）有明显上升；TG变化不明显。结论 在日本血吸虫成虫细胞培养液中适当增加Arg、Thr、Met、Lys和Gluc，同时减少Asp和Ala的含量，及时更换培养液有利于维持血吸虫细胞生长。

报告日本血吸虫成虫培养细胞的超微结构。在透射电镜下日本血吸虫成虫培养细胞呈多角形、圆颗粒形、三角扇形和鞭毛形等多种形状，其中以多角形为主。细胞表面光滑，或有乳头状突起、微绒毛和微饮泡等。胞质内有不同数量的线粒体、内质网、核糖体和糖原颗粒等分布，高尔基复合体很少或无；其中线粒体超微结构的变化可以作为评判培养条件优劣的一个指标。核常呈圆形，核膜为一单位膜，核孔清晰；核内具较丰富的异染色质，核膜内缘常有块状异染色质分布；核仁圆形。不同来源的细胞具不同的内部特征，生殖细胞内核质比例较高，胞质内具许多大小不等的囊泡和不同类型的皮质颗粒；卵黄细胞的典型特征是胞质中存在由高电子密度颗粒组成的卵黄球、卵黄滴；焰细胞的胞质中具纤毛束；神经细胞内具丰富的圆形或椭圆形突触小泡。可识别来源的这些培养细胞中卵黄细胞的数量最多，神经细胞的数量最少。

目的 研究日本血吸虫成虫培养细胞琥珀酸脱氢酶（SDH）和乳酸脱氢酶（LDH）含量、分布及变化规律，了解日本血吸虫成虫培养细胞的能量代谢类型。方法 将虫龄26d的日本血吸虫成虫细胞接种于小盖玻片上，培养于RPMI-1640含20%小牛血清附加常量抗生素的培养基中，定时运用Pearson法进行SDH和LDH染色，用Olympus-BH2显微镜观察并拍照，用HPIAS-2000图像分析仪测量其含量，并作统计分析。结果 日本血吸虫成虫培养细胞均具有SDH 和LDH 活性，两者均分布在细胞质内。培养1d细胞的SDH和LDH 活性最强，随着培养时间延长，其活性逐渐减弱，其中SDH活性下降较快，培养5d大部分细胞SDH活性已极弱；而LDH活性下降则较缓，培养56d细胞仍具LDH活性。结论 体外培养的日本血吸虫成虫细胞的能量代谢类型与成虫相似，既存在三羧酸循环需氧型呼吸链，也具有无氧糖酵解，但以无氧糖酵解为主。

Objective To study the developing cercaria of Schistosoma Japonicum (S. japonicum) on ultrastructural level by transmission electron microscope (TEM) for analyzing the morphological dynamic changes of the tegument, glands and musculature. Methods Artificial infected Oncomelania hupensis were dissected under dissecting microscope and the daughter sporocysts picked up for studying the germinal cells stage (S1). The other embryonic cercaria were selected according to the modified parameter of Chen and Bier (1972). The specimens were prepared by conventional procedure of the laboratory of TEM and were observed by Hitachi H600. Results Besides the germinal cell stage (S1), this is the first chronological study on the morphological development of S. japonicum cercaria from S22S5 concerning the tegument and its elements (glycocalyx, sensory papilla, basal lamina and spine), head and acetabular gland and musculature of the body and tail. This article discusses the ultrastructural morphological differences from prior authors and emphasizes on the postacetalar gland and the pattern of tail musculature of the matured stage cercaria. Conclusions According to the cell division and differentiation, the process of development may be divided into 5 stages: S1, the single germinal cell stage; S2, the germinal ball stage; major multiplication and minor differentiation; S3, tail budding embryonic stage, both active in multiplying and differentiating; S4, the pre-matured stage, major differentiation and minor multiplication; and S5, the fully matured stage (completing the differentiation).