目的 研究实验动物恒河猴在经受抛物线型高+Gx过载后，大脑顶叶皮层和海马区在光镜和电镜下的病理形态学变化，以期深入了解不同高+Gx与脑组织的病理性损伤程度的关系。方法 实验选用健康恒河猴于清醒状态下，在动物离心机上经受相应峰值（实验组：+15Gx/200s、+18Gx/165s、+21Gx/140s和对照组+1Gx/200s）的抛物线型过载作用后，取材、进行病理形态学研究。结果1） 在光镜下，可见大脑顶叶皮层和海马区锥体细胞均表现为不同程度的缺血性萎缩，而星形胶质细胞核周出现空晕，这种病变随着+Gx值的增加而逐渐加重，此外，海马区较大脑顶叶皮层的病变表现的更为明显；2） 电镜下随着+Gx值的增加，可见锥体细胞核染色质聚集形成团块状、染色质边集，核膜皱缩，最后形成凋亡小体；胞浆内线粒体由肿胀、嵴模糊不清最后到空泡化；内质网池逐渐扩张。结论 +15～+21G的高+Gx值可以导致恒河猴脑组织出现急性损伤性病理变化，而且损伤的程度随+Gx的增加而加重。

A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the ex2 plant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell morphology and dynamic growth, analysis of karyotype and isoenzymes of lactate dehydrogenase and malate dehydrogenase were carried out. The expression of recombinant green fluorescence protein in the cells were also undertaken. The results showed that the population doubling time (PDT) of the cells was 24h; the frequency of cell chromosome number to be 2n=64 was 9219%; the banding patterns of the isozymes of the two enzymes had significant difference between the Yunnan pony ear marginal fibroblast cell line and the fibroblast cell lines of PEM 2/2, MSHEM 2/2 and BLCHE 2/2 derived from the Picdmont bovine ear, Mongolian ovine ear and Beijing local chicken embryo respectively. Tests for the contamination from bacteria, fungi or mycoplasma were negative; the transfection efficiency for the recombinant plasmid was 3213%. This newly established cell line make the Yunnan pony breed, a national important genetic resource preserved at cell level, as well as will provide an effective experimental material for genetic studies on the Yunnan pony

The open reading frame of peacock and parakeet prion protein (PrP) genes was cloned and sequenced. The peacock and parakeet PrP genes consisted of 833 and 866 nucleotides encoding 266 and 277 amino acids, respectively (GenBank Accession numbers AY365065 and AY365066). Sequence analysis showed that the peacock and parakeet PrP genes had 93.67% homology to each other, 94.04% and 99.64% homology to the chicken PrP gene and 46.0% and 42.1% similarity to the mammalian PrP genes, respectively. The structural features of all known mammalian and avian PrPs, including N-terminal signal peptides, tandem repeats, conserved hydrophobic region, disulfide bridges and glycoinositol phospholipids anchor, were also found in peacock and parakeet PrPs. The parakeet and peacock PrPs, however, differed in the hexarepeat region, with the peacock having six and the parakeet having seven hexarepeats. The phylogenetic analysis showed that the PrP genes in 52 species were clustered into 2 distinct lineages, the avian and the mammalian. The peacock and parakeet PrP genes belonged to the same lineage but the peacock PrP was sub-classed with the pigeon PrP and the parakeet PrP was sub-classed with the duck and chicken PrPs. The present work added two more species data to the collection of the PrP genes and supported the previous findings that the PrP genes are highly conserved across species.

为了评价生物敷料对烧伤创面的治疗效果，将SPF猪皮细化处理后制成软膏制剂，以其作为生物敷料对大鼠深Ⅱ度烧伤进行了治疗试验，对照组采用普遍油纱布敷料处理，观察烧伤大鼠创面愈合程度，记录愈合时间，并测定了大鼠在烧伤前后的血液学指标。结果显示，试验组比对照组创面愈合时间短，愈合率高，血液学指标恢复较快。