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程树培, CHENG Shupei, ZHONG Zhihui, LU Peng, CHEN Jun, ZHU Jianzhong, JUE Zilong
Journal of Envi ronmental Sciences Vol. 12, No.4, pp. 394-397, 2000,-0001,():
-1年11月30日
The fungi Phanerochaete chrysospori um (PC) and S accharomyces cerevisiae Y99 and the native bacterium YZ1 were the three parental strains for construction of hybrid cells through protoplast fusion to degrade terephthalic acid (TPA) wastewater. The results showed that the native bacterium YZ1 rotoplasm could integrate with that of PC to form the hybrid cell Fhh and the fungus Y99 protoplasm also could integrate with that of Fhh to form the hybrid cell Fhhh. The protoplasts of YZ1 and Y99 could change the morphology of PC spore and mycelium for two times. The hybrid cell Fhhh got the best growth and degradation abilities in the wastewater. It suggested that the hybrid strains obtained from the inter2kingdom protoplast fusion of the three parental strains could create potential for the purification of TPA wastewater.
Phanerochaete chrysospori um, YZ1 bacterium, Saccharomyces cerevisiae, protoplast fusion, terephthalic acid wastewater
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【期刊论文】Purified terephthalic acid wastewater biodegradation and toxicity
程树培, ZHANG Xu-xiang, WAN Yu-qiu, CHENG Shu-pei*, SUN Shi-lei, ZHU Cheng-jun, LI Wei-xin, ZHANG Xiao-chun, WANG Gui-lin, LU Jian-hua, LUO Xiang, GU Ji-dong
,-0001,():
-1年11月30日
The biodegradation and toxicity of the purified terephthalic acid (PTA) processing wastewater researched was at NJYZ pilot with the fusant strain Fhhh in the carrier activated sludge process (CASP). Sludge loading rate (SLR) for Fhhh to COD of the wastewater was 1.09d-1 and to PTA in the wastewater was 0.29d-1. The results of bioassay at the pilot and calculation with software Ebis3 showed that the 48h-LC50 to Daphnia magna for the PTA concentration in the wastewater was only 1/10 of that for the pure chemical PTA. There were 5 kinds of benzoate Pollutants and their toxicities existing in the wastewater at least. The toxicity parameter value of the pure chemical PTA cannot be used to predicate the PTA wastewater toxicity. The toxicity of the NJYZ PTA wastewater will be discussed in detail in this paper.
fusant strain, purified terephthalic acid, wastewater, biodegradation, toxicity, software
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程树培, S.P. Cheng, , Y.B. Liu, Y.B. Cui, S.R. Ding, Y.Z. Shi
Bull. Environ. Contam. Toxicol. (1996) 57: 972-978,-0001,():
-1年11月30日
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程树培, Cheng Shupei, Cui Yibin, Dai Lemei
Journal of Envi ronmental Sciences Vol. 10, No.3, pp. 365-371, 1998,-0001,():
-1年11月30日
The optimal pH and temperature, maximum specific degradation rate, half rate constant and flocculation rate for the hybrid cell Foaz were measured in the reaction for the degradation of soybean processing wastewater (SPW) in this study. The optimal pH and temperature for Foaz in SPW were the same as those of its parental strains S accharomyces cerevisiae Y9407 and Rhodobacter sphaeroi des P9479, but the flocculation rate, the maximum specific degradation rate and the half rate constant for Foaz were higher than those of its parental strains. The results suggest that the characteristics of the inter2kingdom fusant Foaz constructed from the protoplast fusion between the eukaryote cell yeast S. cerevisiae and the prokaryote cell photosynthetic bacteria R. sphaeroi des may favor degrading organic pollutant in SPW and removing biomass from the effluent.
Saccharomyces cerevisiae, Rhodobacter sphaeroi des, soybean processing, wastewater
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程树培, Z.T. Liu, , L.S. Wang, S.P. Chen, W. Li, H.X. Yu
Bull. Environ. Contam. Toxicol. (1996) 57: 421-425,-0001,():
-1年11月30日
The fundamental differentiation of toxicity is between reactive and nonreactive toxicity. Reactive toxicity is associated with a specific mechanism for the reaction with an enzyme or inhibition of a metabolic pathway, and nonreactive toxicity is related directly to the quantity of toxicant acting upon the cell. The quantitative structure-activity relationships (QSARs) have been successfully used in the nonreactive toxicity, such as prediction of the toxicity of nonreactive compounds based on their solubility in the lipids of organisms (Mackay 1988; Yoshioka et al. 1986). The elements of molecular structure that are most closely related to nonreactive toxicity are those that describe the partitioning of the toxicant into the organism (Blume and Speece 1990), while QSARs for the reactive toxicity are less common in the environmental toxicology literature. With the recent increase in the use of synthetic substituted benzenes as industrial chemicals, the accurate analysis of the effect of reactive toxic chemicals has become recognized with QSAR (Nendza et al. 1988). For this purpose, we selected the fish (Carassias auratus) as the test organism, measured the acute toxicity of 50% lethal concentration (LC50) of the chemicals and the adenosine triphosphate (ATP) content of the liver cells for the organism. These determined the relationships of the acute toxicity of some substituted benzenes with their physicochemical structural parameters. The effects on the ATP content was also compared to predict biological reactivities of the chemicals, so as to find some clues to explain the mode of mechanism of the toxicity.
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