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【期刊论文】A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori)
王俊
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-1年11月30日
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【期刊论文】RePS: A Sequence Assembler That Masks Exact Repeats Identified from the Shotgun Data
王俊, Jun Wang, , Gane Ka-Shu Wong, Peixiang Ni, Yujun Han, Xiangang Huang, Jianguo Zhang, Chen Ye, Yong Zhang, Jianfei Hu, Kunlin Zhang, Xin Xu, Lijuan Cong, Hong Lu, Xide Ren, Xiaoyu Ren, Jun He, Lin Tao, Douglas A. Passey, Jian Wang, Huanming Yang, Jun Yu, and Songgang Li
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-1年11月30日
We describe a sequence assembler, RePS (repeat-masked Phrap with scaffolding), that explicitly identifies exact 20mer repeats from the shotgun data and removes them prior to the assembly. The established software Phrap is used to compute meaningful error probabilities for each base. Clone-end-pairing information is used to construct scaffolds that order and orient the contigs. We show with real data for human and rice that reasonable assemblies are possible even at coverages of only 4
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【期刊论文】Neutral evolution of 'non-coding' complementary DNAs
王俊, Jun Wang*†, Jianguo Zhang†, Hongkun Zheng†, Jun Li†, Dongyuan Liu†, Heng Li†, Ram Samudrala‡, Jun Yu*†, Gane Ka-Shu Wong*†§
Y. Okazaki et al. Nature 420, 563-573 (2002),-0001,():
-1年11月30日
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王俊, Le Kang*†, XiangYong Chen*, Yan Zhou‡, BoWan Liu*, Wei Zheng*, RuiQiang Li‡, Jun Wang‡, and Jun Yu‡
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-1年11月30日
The migratory locust is one of the most notorious agricultural pests that undergo a well known reversible, density-dependent phase transition from the solitary to the gregarious. To demonstrate the underlying molecular mechanisms of the phase change, we generated 76,012 ESTs from the whole body and dissected organs in the two phases. Comparing 12,161 unigene clusters, we identified 532 genes as phase-related (P<0.01). Comprehensive assessment of the phase-related expression revealed that, whereas most of the genes in various categories from hind legs and the midgut are down-regulated in the gregarious phase, several gene classes in the head are impressively up-regulated, including those with peptidase, receptor, and oxygen-binding activities and those related to development, cell growth, and responses to external stimuli. Among them, a superfamily of proteins, the JHPH superfamily, which includes juvenile hormone-binding protein, hexamerins, prophenoloxidase, and hemocyanins, were highly expressed in the heads of the gregarious hoppers and hind legs of the solitary hoppers. Quantitative PCR experiments confirmed in part the EST results. These differentially regulated genes have strong functional implications that numerous molecular activities are involved in phase plasticity. This study provides ample molecular markers and genomic information on hemimetabolous insects and insights into the genetic and molecular mechanisms of phase changes in locusts.
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【期刊论文】Relationship between dynamic resonance frequency and egg physical properties
王俊, J. Wang*, R.S. Jiang, Y. Yu
Food Research International 37(2004)45-50,-0001,():
-1年11月30日
An experimental system was set up to generate the impact force, measure the response wave signal, and analyse the frequency spectrum for physical property detection of biological product. The egg dynamic resonance frequency was obtained through the analysis of the dynamically measured frequency response of an egg excited by pendulum. The effects of excitation point, detected point, impact intensity, excitation material, egg mass, egg density, shell stiffness and shell crack on the dominant frequency were analysed. The results showed that the dominant frequency was significantly affected by the shell crack, shell stiffness, egg mass and egg density, and not significantly affected by excitation point, detected point, excitation velocity and impacting material. The dominant frequency increased with increasing of shell stiffness or egg density, and decreased with increase of the egg mass. The coefficient of determination (R2) of the quadratic relationship between the dominant frequency and the three egg physical properties was 0.62-0.68.
Egg, Physical property, Dynamic, Resonance frequency
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