AIM: To determine the effect of apoptosis on gastric cancercells (SGC-7901) induced by cis-9, trans-11-conjugatedlinoleic acid (c9, t11-CLA) and its possible mechanism in theinhibition of cancer cells growth. METHODS: Using cell culture, flow cytometery andimmunocytochemical techniques, we examined the cellgrowth, frequency of apoptosis and distribution of cell cycle,expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cellswhich were treated with various c9, t11-CLA concentrations(25,50,100 and 200 mmol

AIM:To investigate the effect of c9,t11-conjugatedlinoleic acid (c9,t11-CLA) on the invasion of human gastriccarcinoma cell line and its possible mechanism of preventing metastasis. METHODS: Using reconstituted basement membrane invasion, chemotaxis, adhesion, PAGE substrate zymography and RT-PCR assays, we analyzed the abilities of invasion, direct migration, adhesion of intracellular matrix, as well as the activity of type IV collagenase and expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 mRNA in SGC-7901 cells which were treated with gradually increased concentrations (25, 50, 100 and 200 mmol/L) of c9, t11-CLA for 24h. RESULTS: At the concentrations of 200mmol/L, 100mmol/L and 50 mmol/L, c9,t11-CLA suppressed the invasion of SGC-7901 cells into the reconstituted basement membrane by53.7%, 40.9% and 29.3%, respectively, in comparison with the negative control. Only in the 200 mmol/L c9,t11-CLA group, the chemotaxis of SGC-7901 cells was inhibitedby 16.0% in comparision with the negative control. C9, t11-CLA also could inhibit the adhesion of SGC-7901 cells tolaminin, fibronectin and Matrigel, increase the expressionof TIMP-1 and TIMP-2 mRNA, and reduce type IV collagenaseactivities in the serum-free medium supernatant of SGC-7901 cells.CONCLUSION: c9, t11-CLA can inhibit the invasion of SGC-7901 cells at multiple procedures in tumor metastasiscascade, which may be associated with the induction ofTIMP-1 and TIMP-2 mRNA expression.Chen BQ, Yang YM, Gao YH, Liu JR, Xue YB, Wang XL, ZhengYM, Zhang JS, Liu RH. Inhibitory effects of c9,t11-conjugatedlinoleic acid on invasion of human gastric carcinoma cell lineSGC-7901. World J Gastroenterol 2003;9(9):1909-1914http://www.wjgnet.com/1007-9327/9/1909.asp