采用体外培养技术，模拟体内肿瘤转移过程，研究共轭亚油酸（conjugated linoliec acid, CLA）对鼠黑色素瘤Bl6-MB高转移细胞系的转移特性，从而探讨CLA预防肿瘤转移的可能途径。研究结果表明，剂量为lOOUmol/L、200Umol/L的CLA可抑制肿瘤细胞增殖、恢复肿瘤细胞间通讯功能、抑制肿瘤细胞向细胞外基质成分[层粘连蛋白（fibronectin, FN）和纤维粘连蛋白（laminin，I.N）]的黏附。

AIM:To explore the inhibition of conjugated linoleic acidisomers in different purity (75% purity c9, t11-, 98% purityc9,t11- and 98% purity t10, c12-CLA) on the formation offorestomach neoplasm and cheopreventive mechanisms. METHODS: Forestomach neoplasm model induced by B(a)P in KunMing mice was established. The numbers of tumorand diameter of each tumor in forestomach were counted;the mice plasma malondialdehyde (MDA) were measuredby TBARS assay; TUNEL assay was used to analyze theapoptosis in forestomach neoplasia and the expression ofMEK-1, ERK-1, MKP-1 protein in forestomach neoplasm werestudied by Western Blotting assay.RESULTS: The incidence of neoplasm in B(a)Pgroup, 75%purity c9, t11-CLA group, 98% purity c9,t11-CLA groupand 98% purity t10, c12-CLA group was 100%, 75.0 %(P>0.05), 69.2%(P<0.05) and 53.8%(P<0.05) respectivelyand the effect of two CLA isomers in 98% purity onforestomach neoplasia was significant; CLA showed noinfluence on the average tumor numbers in tumor-bearingmouse, but significantly decreased the tumor size, the tumor average diameter of mice in 75% purity c9,t11-CLA group,98% purity c9,t11-CLA group and 98% purity t10, c12-CLAgroup was 0.157

目的 研究c9t11-共轭亚油酸（CLA）对人胃腺癌细胞（SGC-7901）侵袭能力的影响，探讨其抑制肿瘤转移的可能机制。方法 用重组基底膜侵袭实验评价癌细胞侵袭能力；用逆转录聚合酶链反应（RT-PCR）检测SGC-7901 细胞中组织基质金属蛋白酶抑制剂（TIMP）-1、TIMP-2 和nm23-H1mRNA 的表达。结果 在200、100和50μmol/L浓度时，c9t11-CLA 对SGC-7901细胞侵袭重组基底膜的抑制率分别为53.7%、40.9%和29.3%。c9，t11-CLA可诱导SGC-7901细胞中TIMP-1、TIMP-2和nm23-H1 mRNA 的表达。结论 c9t11-CLA 抑制SGC-7901细胞侵袭重组基底膜。c9t11-CLA 的抗侵袭活性与诱导肿瘤细胞中TIMP-1、TIMP-2 和nm23-H1 mRNA的表达等有关。

AIM: To determine the effect of apoptosis on gastric cancercells (SGC-7901) induced by cis-9, trans-11-conjugatedlinoleic acid (c9, t11-CLA) and its possible mechanism in theinhibition of cancer cells growth. METHODS: Using cell culture, flow cytometery andimmunocytochemical techniques, we examined the cellgrowth, frequency of apoptosis and distribution of cell cycle,expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cellswhich were treated with various c9, t11-CLA concentrations(25,50,100 and 200 mmol