The rice heat blast process is a novel technique for gelatinizing raw starch. It uses heated air to replace steam for processing rice under high temperature fluidization in a short time period. This is a new technique for making rice wine with the characteristics of easy storage and zero water pollution. This study focused on three major performance indexes (starch gelatinization ratio, total fat content, and amino nitrogen content in the roasted rice), which largely affect the performance of the rice heat blast process and the rice wine quality. The relationship between these performance indexes and the corresponding operation variables were modeled by an artificial neural network (ANN) via learning sets of experimental data. Based on the ANN models obtained, genetic algorithms were used to optimize the operating conditions of the rice heat blast process. The results showed the power in determination of optimal operating conditions by the combinational utilization of artificial neural network and genetic algorithms.

Using Saccharomyces cerevisiae, a novel, repeated fed-batch ethanol production system from corn flour by fully recycling fermented supernatants is demonstrated. With recovery of ethanol by evaporation coupled with consecutive removal of the insoluble and soluble inhibitory substances accumulated, either completely or partially by filtration, the concentrations of the soluble inhibitors in the system could be maintained at their equilibria. As a result, a sustained high concentration of ethanol (up to 15% v/v) and significant pollution control performance were obtained.

研究了酒精蒸馏废液全循环对玉米粉液化、糖化和发酵的影响，结果表明，当液化酶用量达到14U/g、糖化酶用量为120U/g（玉米粉）时，蒸馏废液对液化无影响，而对糖化有促进作用；蒸馏废液能一定程度地促进酵母的生长，提高酵母耗糖速率；蒸馏废液经过25次循环发酵，酒精平均浓度在15.07%，平均糖利用率91.7%。蒸馏废液全循环工艺，解决了发酵法酒精生产中的污染问题，实现了发酵法酒精清洁生产。

A general mathematical model for performance evaluation of acetone-butanol continuous flash extractive fermentation system was formulated in the terms of productivity, energy requirement (energy utilization efficiency) and product purity. The simulation results based on experimental data showed that the most pronounced performance improvement could be achieved by using highly concentrated substrate as feed and the increase in solvent dilution rate could only improve the total productivity at the expense of energy utilization efficiency. A two-vessel partial flash system, with the first vessel of two to three plates and the second vessel as a complete flash vessel, is required to ensure high product purity. This method of mathematical formulation and analysis could be used and extended as a general tool to other bioprocesses equipped with multiple in siturecovery systems.

近年来，1，3-甘油二酯（DAG）由于其广泛用途及健康作用日益受到人们的重视。报道了一种无溶剂条件下填充床反应器中连续酶促合成1，3-DAG的方法。研究了填充柱的长径比、进料体积流速、温度、底物摩尔比对酯化率和1，3-DAG产量的影响。结果表明固定化酶填充柱长径比7.8，亚油酸、甘油摩尔比1:2，进料速度1.2mLPmin，65℃条件下酯化反应可实现脂肪酸酯化率、1，3-DAG纯度及生产效率的统一。填充床反应器中固定化酶连续催化酯化反应的一个主要问题即体系水分清除困难。实验研究了采用过量甘油吸附脱水的可行性，亚油酸、甘油摩尔比为1:2时，可明显改善固定化酶的稳定性，增加LipozymeRMIM的使用寿命。连续运行10d，残余酶活仍保持在80%以上，而对照组则仅为52%。