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罗建平, Jian-Ping Luo*, Shao-Tong Jiang and Li-Jun Pan
Plant Growth Regulation 40: 171-177, 2003.,-0001,():
-1年11月30日
The inter-relationship between exogenous calcium (Ca2+) during cold pretreatment and cold-enhanced somatic embryogenesis was investigated using cell suspension cultures of Astragalus adsurgens Pall. Cell suspension was obtained from embryogenic callus and could be induced to form somatic embryos in the differentiation medium. Suspension cells, after cold-treatment at 8℃ for 2 to 3wk, displayed an enhanced capacity for somatic embryogenesis as compared to those without cold pretreatment. Longer cold pretreatment (>4wk) resulted in the inhibition of somatic embryogenesis. The enhanced embryogenic response of cells to cold pretreatment was dependent on the Ca2+ level in the pretreatment medium. Ca2+ levels below 1 mM suppressed the cold-enhanced response. Addition of lanthanum into the pretreatment medium completely abolished the cold induced enhancement of somatic embryogenesis. These results suggest that embryogenic cells require a minimal concentration of Ca2+ during pretreatment for the expression of this cold-enhanced capacity for somatic embryogenesis in A. adsurgens and the influx of exogenous Ca2+ during pretreatment might also be involved.
Astragalus adsurgens,, Calcium,, Cold pretreatment,, Somatic embryogenesis
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罗建平, Jian-Ping Luo*, Shao-Tong Jiang, Li-Jun Pan
J.-P. Luo et al./Plant Science 161(2001)125-132,-0001,():
-1年11月30日
Salicylic acid (SA), when added to the differentiation medium below 200mol/l, significantly enhanced somatic embryogenesis in callus culture of Astragalus adsurgens Pall. The highest frequency of somatic embryogenesis occurred at 150mol/l SA. Enhanced somatic embryogenesis by SA was accompanied by an increase in the endogenous H2O2 level as compared with controls. This increased endogenous H2O2 level was related to the inhibition of the activities of ascorbate peroxidase (APX) and catalase (CAT). Although the promoting effect of exogenous H2O2 was significantly lower than that of exogenous SA on the development of somatic embryos, the pre-treatment of callus with dimethylthiourea (a trap for H2O2) significantly inhibited somatic embryogenesis, even if callus was cultured on the differentiation medium supplemented with 150
Salicylic acid, Hydrogen peroxide (, H2O2), , Astragalus adsurgens Pall., , H2O2 metabolising enzymes, Somatic embryogenesis
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罗建平, Jian-Ping Luo a, *, Jing-Fen Jia b, Yue-Hua Gu a, Jing Liu a
J.-P. Luo et al./Plant Science 143(1999)93-99,-0001,():
-1年11月30日
Efficient plant regeneration through somatic embryogenesis was achieved in Astragalus adsurgens. Embryogenic callus was induced from hypocotyl, not root or cotyledon explants, and the maximum induction frequency (62%) was obtained on Murashige and Skoog (1962) basal medium (MS) containing 2.0 mg:l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5mg/l N6-benzylaminopurine (BA). Transfer of embryogenic calli to MS medium with 1-2mg/l BA and 0.1mg/l a-naphthaleneacetic acid (NAA) resulted in somatic embryogenesis at high frequencies (63-74%) with an average of 280 somatic embryos per gram of embryogenic callus. After transfer onto half-strength MS medium without growth regulators, approximately 80% of somatic embryos developed into complete plantlets. Chromosome count of root tips from regenerants revealed a normal diploid. The rooted plantlets were successfuly transferred to soil with 60-80% survival, and the plants showed normal morphological characteristics. On MS medium containing 1.0mg/l 2,4-D and 0.5mg/l BA, embryogenic calli have retained morphogenetic potential for 12 months.
Astragalus adsurgens, Embryogenic callus, Forage legume, Plant regeneration, Somatic embryogenesis
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【期刊论文】Protoplast culture and paclitaxel production by Taxus yunnanensis
罗建平, Jian-Ping Luo, *, QingMu & Yue-Hua Gu
Plant Cell, Tissue and Organ Culture 59: 25-29, 1999.,-0001,():
-1年11月30日
Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0mg l-1 2,4-dichlorophenoxyacetic acid and 0.1 mg l-1 kinetin. The planting density was 2.5-3.0
Taxus yunnanensis,, protoplast-derived colonies,, paclitaxel
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