已为您找到该学者9条结果 成果回收站
罗建平, Jian-Ping Luo*, Xue-Qiang Zha & Wei Shi
Plant Cell, Tissue and Organ Culture (2005) 82: 75-81,-0001,():
-1年11月30日
In vitro selection was carried out to obtain ethionine-resistant plants with increased contents of free methionine in the vegetative tissues of the forage legume Astragalus adsurgens Pall. Three-week-old cell colonies were derived from protoplasts mutagenized with N-methyl-N-nitrosoguanidine from embryogenic callus and were selected with 0.6 mM ethionine. Four colony lines were isolated and their resistance to ethionine was 7-8 times that of the wild-type callus. No plant regeneration occurred on these colony lines in the differentiation medium containing ethionine. Only one colony line (R-1) regenerated plants through somatic embryogenesis in the absence of ethionine. Stem and leaf segments from the regenerated plants showed the same potential to produce callus in the presence of ethionine as in the absence of ethionine. The formed callus kept continuously growing in ethionine-containing medium. Free amino acid analysis revealed that colony line R-1, its regenerated plants and callus from the regenerated plants accumulated methionine at levels at 5-9 times higher than in wild-type. These results suggested that ethionine resistance and methionine over-accumulation were also expressed at plant level. Thus, the obtained resistant colony line that could regenerate plants with over-accumulation of methionine might provide an alternative approach to improve the nutritional quality of this forage.
Astragalus adsurgens Pall,, ethionine-resistance,, methionine,, plant regeneration,, protoplasts
-
46浏览
-
0点赞
-
0收藏
-
0分享
-
74下载
-
0
-
引用
罗建平, Jian-Ping Luo*, Xue-Qiang Zha & Wei Shi
Plant Cell, Tissue and Organ Culture (2005)82: 75-81,-0001,():
-1年11月30日
In vitro selection was carried out to obtain ethionine-resistant plants with increased contents of free methionine in the vegetative tissues of the forage legume Astragalus adsurgens Pall. Three-week-old cell colonies were derived from protoplasts mutagenized with N-methyl-N-nitrosoguanidine from embryogenic callus and were selected with 0.6mM ethionine. Four colony lines were isolated and their resistance to ethionine was 7-8 times that of the wild-type callus. No plant regeneration occurred on these colony lines in the differentiation medium containing ethionine. Only one colony line (R-1) regenerated plants through somatic embryogenesis in the absence of ethionine. Stem and leaf segments from the regenerated plants showed the same potential to produce callus in the presence of ethionine as in the absence of ethionine. The formed callus kept continuously growing in ethionine-containing medium. Free amino acid analysis revealed that colony line R-1, its regenerated plants and callus from the regenerated plants accumulated methionine at levels at 5-9 times higher than in wild-type. These results suggested that ethionine resistance and methionine over-accumulation were also expressed at plant level. Thus, the obtained resistant colony line that could regenerate plants with over-accumulation of methionine might provide an alternative approach to improve the nutritional quality of this forage.
Astragalus adsurgens Pall,, ethionine-resistance,, methionine,, plant regeneration,, protoplasts
-
31浏览
-
0点赞
-
0收藏
-
0分享
-
98下载
-
0
-
引用
【期刊论文】Protoplast culture and paclitaxel production by Taxus yunnanensis
罗建平, Jian-Ping Luo, *, QingMu & Yue-Hua Gu
Plant Cell, Tissue and Organ Culture 59: 25-29, 1999.,-0001,():
-1年11月30日
Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0mg l-1 2,4-dichlorophenoxyacetic acid and 0.1 mg l-1 kinetin. The planting density was 2.5-3.0
Taxus yunnanensis,, protoplast-derived colonies,, paclitaxel
-
30浏览
-
0点赞
-
0收藏
-
0分享
-
93下载
-
0
-
引用
【期刊论文】Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall.
罗建平, J.-P. Luo • J.-F. Jia
Plant Cell Reports (1998)17: 313-317,-0001,():
-1年11月30日
A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens. Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5mg/l 2,4-D, 0.5mg/l BA and 0.5 Mglucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems showing a plating efficiency of 0.8
Astragalus adsurgens • Protoplast culture • Plant regeneration • Embryogenesis
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
44下载
-
0
-
引用
罗建平, Jian-Ping Luo a, *, Jing-Fen Jia b, Yue-Hua Gu a, Jing Liu a
J.-P. Luo et al./Plant Science 143(1999)93-99,-0001,():
-1年11月30日
Efficient plant regeneration through somatic embryogenesis was achieved in Astragalus adsurgens. Embryogenic callus was induced from hypocotyl, not root or cotyledon explants, and the maximum induction frequency (62%) was obtained on Murashige and Skoog (1962) basal medium (MS) containing 2.0 mg:l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5mg/l N6-benzylaminopurine (BA). Transfer of embryogenic calli to MS medium with 1-2mg/l BA and 0.1mg/l a-naphthaleneacetic acid (NAA) resulted in somatic embryogenesis at high frequencies (63-74%) with an average of 280 somatic embryos per gram of embryogenic callus. After transfer onto half-strength MS medium without growth regulators, approximately 80% of somatic embryos developed into complete plantlets. Chromosome count of root tips from regenerants revealed a normal diploid. The rooted plantlets were successfuly transferred to soil with 60-80% survival, and the plants showed normal morphological characteristics. On MS medium containing 1.0mg/l 2,4-D and 0.5mg/l BA, embryogenic calli have retained morphogenetic potential for 12 months.
Astragalus adsurgens, Embryogenic callus, Forage legume, Plant regeneration, Somatic embryogenesis
-
37浏览
-
0点赞
-
0收藏
-
0分享
-
147下载
-
0
-
引用