Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in wa-ter-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we devel-oped the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection effi-ciency, low cell toxicity and biodegradability.

不同生长素对盾叶薯蓣（Dioscorea zingiberensis C.H.Wright）愈伤组织细胞生长量的影响差异是NAA>IAA>2，4D，而对薯蓣皂甙元合成的影响是2，4 D>IAA>NAA。在有生长素存在的条件下，光照24h，另加05mg LKT促进愈伤组织细胞的生长，而附加05mg LBA促进薯蓣皂甙元的合成；相反，光照12h，另加05mg LBA促进生长，附加5mgLKT促进合成。愈伤组织生长越旺，薯蓣皂甙元积累越少。低浓度的生长素促进薯蓣皂甙元的合成。

用低能N+离子束注入拟南芥后获得的稳定突变体T80Ⅱ作为实验材料，对突变体植株进行了RAPD标记，并将T80Ⅱ和对照部分RAPD特异条带进行克隆测序和DNA序列分析。结果显示、在可分辨的总计397个RAPD条带中，T80Ⅱ株系中有52个条带表现出差异，包括条带的缺失和增加，条带变异率为13.1%；克隆的T80Ⅱ序列中，平均每16.8个碱基出现1个碱基变异位点，表现出较高频率的碱基突变。碱基突变类型包括碱基的颠换、转换、缺失、插入等。在检测到的275个碱基突变中，主要是单碱基置换（97.09%），碱基缺失或者插入的比例较小（2.91%）。在碱基置换中，转换的频率（66.55%）高于颠换的频率（30.55%）。此外，构成DNA的4种碱基均可以被离子束辐照诱发变异，而且每一种碱基都可以被其他3种碱基所替换，但是胸腺嘧啶（T）的辐射敏感性要高于其他3种碱基。通过分析突变碱基周边序列，对低能N+离子注入拟南芥突变体引发的碱基突变热点进行了讨论。

The electrochemical behavior of breast cancer cells was studied on a graphite electrode by cyclic voltammetry (CV) and potentiometric stripping analysis (PSA). In both cases, only one oxidative peak at approximately +0.75V was observed. The peak area in PSA was used to study the growth of the cells and the effect of diosgenin on MCF-7 cells. The results showed that diosgenin can effectively inhibit the viability and proliferation of the breast cancer cells.

用水稻幼穗为外植体进行组织培养，研究了影响其体细胞胚胎发生的有关因素，建立了高频率体细胞胚胎发生的培养程序。结果表明不同基因型之间体细胞胚胎发生率的差异达到612%；生长素2，4D对诱导水稻体细胞胚起重要的调控作用，细胞分裂素BA有一定的协同促进作用。干燥处理可提高愈伤组织体细胞胚胎发生率，愈伤组织含水量在60%～80%范围的培养效果较好；外源DNA溶液浸泡发育早期的心状体细胞胚，其进一步发育时异常胚的频率显著增加，成苗率降低。