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2009年09月27日

【期刊论文】野生扬子鳄生境特征分析

吴孝兵, 吴陆生, 吴孝兵*, 江红星, , 王朝林

生物多样性,2005,13(2):156~161,-0001,():

-1年11月30日

摘要

作者分别于2002和2003年抽样调查了安徽省扬子鳄国家级自然保护区有或曾经有野生扬子鳄(Alligatorsinensis)分布的22个样地,选择了与野生扬子鳄生存有关的8类生态因子,即水域中岛屿情况、水域水面的稳定度、水体pH值、螺类丰富度、岸线植被盖度、岸线土壤质地、苦竹密度和植被类型,运用资源选择函数结合主成分分析方法研究了野生扬子鳄对生境的选择。结果表明岸线植被盖度对野生扬子鳄的生境选择影响最大,其次是水体pH值,再次是螺类丰富度、苦竹密度、水域水面的稳定度和土壤质地;而水域中岛屿情况与植被类型对野生扬子鳄生境选择的影响则较弱。

Alligator sinensis, 生境特征, 资源选择函数, 主成分分析

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2009年09月27日

【期刊论文】从12SrRNA和Cytb基因部分序列研究13种猫科动物的分子系统关系*

吴孝兵, 郑涛, 费荣梅, 吴孝兵*

动物学报,2005,51(4):630~639,-0001,():

-1年11月30日

摘要

为探讨中国猫科动物(Felidae)的系统发生关系,本文对中国产13种猫科动物的12SrRNA基因(约371bp)和细胞色素b基因(Cytb)部分序列(约355bp)进行了分析,并采用“最大简约法”和“最大似然法”构建了分子系统树。结果表明:在Cytb基因序列中,有113个位点存在变异(约为总位点数的31.8%),高于12SrRNA基因序列的44个变异位点(约为总位点数的11.9%);构建的分子系统树显示,猞猁(Lynxlynx)可能是中国最早起源的猫科动物,与其它猫科动物之间的亲缘关系较远,支持将其立为猞猁属(Lynx)的观点;草原斑猫(Felislibyca)、丛林猫(Felischaus)、兔狲(Otocolobusmanu1)和荒漠猫(Felisbieti)具有较近的亲缘关系,支持将兔狲划归于猫属(Felis)的观点;金猫(Caopumatemminckii)、云猫(Pardofelismarmorata)具有较近的亲缘关系,但它们与猫属物种之间的亲缘关系可能较远,不支持将它们划归于猫属:豹猫(Ponailurusribengalensis)、渔猫(Prionailurusviverrinus)具有较近的亲缘关系,支持将它们同归于豹猫属(Ponailurus);云豹(Neofelisnebulosa)、豹(Pantherapardus)、雪豹(Unciauncia)、虎(Pantheratigris)具有较近的亲缘关系,支持将它们同归于豹属(Panthera)的观点[动物学报51(4):630-639,2005]。

中国, 猫科动物, 12SrRNA基因, Cytb基因, 系统发生

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2009年09月27日

【期刊论文】夏秋两季饲养条件下扬子鳄的行为谱和活动节律初步研究

吴孝兵, 张方, 朱家龙, 章松

水生生物学报,2005,29(5):488~494,-0001,():

-1年11月30日

摘要

2003年夏秋两季(7-11月间),采用扫描取样法和目标动物取样法,对饲养条件下扬子鳄的个体行为和活动节律进行了研究。结果表明夏秋两季饲养条件下扬子鳄的个体行为主要包括休息行为(水中休息、陆地休息、晒太阳、挠痒、打哈欠、伸懒腰)运动行为(游泳、潜水、爬行、摆尾);摄食行为(摄食、漱口);冲突行为(打斗、对视);嬉戏行为(嬉戏、堆积);吼叫行为;排泄行为等。夏季,扬子鳄休息行为的时间最多,日变化规律显示;早晨6:00-7:00为休息高峰期;下午12:30-13:30为潜水高峰期。秋季,用于晒太阳的时间最多,秋季早展6:00-7:30为潜水高峰期,且一天中扬子鳄晒太阳活动出现三次高峰即10:30-11:00,13:30-14:00,16:30-17:00。统计结果表明,夏秋两季潜水、晒太阳和休息3种个体行为差异性较为显著。在弋30℃-36℃之间,扬子鳄潜水时间和温度之间呈线性关系。研究结果同时表明长期人工饲养对扬子妈的某些弹性行为如摄食行为等产生了较大影响。在行为描述的基础上,对扬子鳄的有关行为机制进行了探讨。而影响扬子鳄行为表达的生物因素主要包括年龄和食物资源非生物因素主要包括空间和生境等。

扬子鳄, 行为, 活动节律

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2009年09月27日

【期刊论文】棘胸蛙消化道内分泌细胞的免疫组织化学定位*

吴孝兵, 张盛周, 黄徐根, 吴孝兵**

动物学报,2003,49(6):858~864,-0001,():

-1年11月30日

摘要

We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin-peroxidase method) using eight gut hormone antisera. The 5-hydroxytryptamine immunoreactive cells were distributed throughout the digestive tract with the highest density in the stomachus pyloricus, the second highest in the duodenum, fewer in the oesophagus, stomachus cardiacus and rectum. The gastrin immunoreactive cells were located mainly in the stomachus pyloricus and occasionally in different parts of the intestine. The somatostatin immunoreactive cells occured mainly in the stomach, frequently in the stomachus pyloricus, and occasionally in different parts of the intestine. The pancreatic polypeptide immunoreaetive cells were found with the highest density in the duodenum, the second highest in the stomachus cardiacus, and rarely in the rectum. No immunoreactive cells were observed with the antisera to glucagon, substance P, growth hormone and calcitonin. but there were glucagon and substance P mucosal nerve plexus throughout the digestive tract, and both with the highest density in the duodenum [Acta Zoologica Sinica 49(6): 858 - 864, 2003].

棘胸蛙, 消化道, 内分泌细胞, 免疫组织化学

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2009年09月27日

【期刊论文】Genetic variation in captive population of chinese alligator, Alligator sinensis, revealed by random amplified polymorphic DNA (RAPD)

吴孝兵, Xiao-Bing Wu a, b, *, Yi-Quan Wang a, Kai-Ya Zhou a, Wei-Quan Zhu a, Ji-Shan Nie c, Chao-Lin Wang c, Wan-Shu Xie c

Biological Conservation 106(2002)435-441,-0001,():

-1年11月30日

摘要

Chinese alligator (Alligator sinensis) is an endemic species in China. The likely extinction of it in the wild has been recognised. To prevent this species becoming extinct, the Anhui Research Centre of Chinese Alligator Reproduction (ARCCAR) was established in Xuanzhou, Anhui Province in 1979, where has been established the largest captive population of Chinese alligator (XZSP) in the world. Another farm (CXSP) was established by villagers in Changxin, Zhejiang Province. The results of an investigation of the two captive subpopulation structures by genetic analysis are presented in this paper. We examined the genetic variation in the two captive subpopulations using RAPDs. Thirty-one random primers were selected among 199 random primers screened. A total of 193 reproducible RAPD fragments were scored among 43 individuals, of which 21 (10.88%) were polymorphic. The genetic distances between 43 individuals ranged from 0 to 0.0376 with average of 0.0104 0.0055 S. E. The genetic similarity in CXSP (0.9948 0.0029 S. E.) was higher than that in XZSP (0.9894 0.0055 S.E.). The founder effect is a possible explanation for very low genetic variation in CXSP. Analysis of the RAPD data showed that the mean phenotypic band frequencies of each polymorphic loci was 0.6656 0.3730 S. E. The lowest phenotypic band frequency (0.0233) was found in four of those polymorphic loci. There was no genetic difference between the two subpopulations (Dij=0.0009). According to the dendrogram and the distribution of polymorphic fragments in two subpopulations, CXSP originated genetically from XZSP. This paper summarises a preliminary research on genetic structure in populations of Chinese alligator. Although there is higher genetic similary (0.9896 0.0055 S. E.) in captive population of A. sinensis, we did not determine whether or not loss of genetic variation had occurred in relation to a wild control population. The data of malformed offspring will be collected carefully, and wild samples be added to set up a control population in future study.

Alligator sinensis, RAPD, Genetic variation, Ex sito conservation

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    安徽师范大学,安徽

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