目的 研究免疫调节剂咪喹莫特对卵蛋白（OVA）致敏大鼠支气管旁淋巴结（PBLN）细胞培养体系中T辅助淋巴细胞（Th）亚群产生细胞因子的影响，并探讨其作用机制。方法 建立PBLN细胞培养体系，并按不同浓度分为A～F组进行干预。在培养0、3、6、12、24、48h后，各组分别取5孔细胞培养液，用酶联免疫吸附法（ELISA）检测各细胞因子的蛋白质水平，用逆转录聚合酶链反应（RT-PCR）检测细胞沉淀中白细胞介素（IL）-4和干扰素（IFN）-γ等细胞因子的mRNA表达。结果 在A组的各时间点PBLN细胞培养液中，仅可检测到少量IFN-γ。随着培养时间的延长，咪喹莫特浓度为1、10μg/ml的E和F组与B组相比，IL-4及相关mRNA水平增加缓慢，IFN-γ水平增长迅速（均P <0.005）。此作用从培养6h开始，12h达峰值，持续至24h。在培养12-48h时，C组IL-4表达与B组比，差异有统计学意义（P<0.05），而IFN-γ表达无差异（P>0.05）。结论 咪喹莫特对致敏大鼠PBLN细胞培养体系中Th亚群产生细胞因子的最佳作用发生在12h时，提示咪喹莫特可能在支气管哮喘等特异性疾病的迟发炎症反应阶段发挥重要作用。

Background; As a result of the finding that the mutation of Arg into Gly at β2-adrenergic receptor(β2-AR)16 loci could promote the downregulation effect triggered by the β2-agonist, it was supposed that Gly16 might be associated with the downregulation of β2-AR in patients with nocturnal asthma. Objective; It was the aim of this study to analyze the association between β2-AR genetic polymorphisms and nocturnal asthmatic patients of Chinese Han nationality. Methods; A polymerase chain reaction allele-specifi c oligonucleotide hybridization assay was used to determine 16 and 27 loci alleles of β2-AR genetic polymorphisms in 25 nocturnal asthmatic patients(nocturnal asthma group), 22 non-nocturnal asthmatic patients(non-nocturnal asthma group), and 72 healthy people(control group). All people investigated were of Chinese Han nationality. Results; The distribution frequency of genotype Arg/Arg, Arg/Gly, and Gly/Gly at β2-AR 16 loci was 12, 16 and 72% in the nocturnal asthma group; and 27, 41 and 32% in the non-nocturnal asthma group. There was a signifi cant increase in the frequency of genotype Gly/Gly and allele Gly in the nocturnal asthma group compared with the non-nocturnal asthma group(p<0.01). However, there was no signifi cant difference in the frequency of genotype Gly/Gly and allele Gly in the non-nocturnal asthma group, compared withthe control group. There was no signifi cance in the frequency of the genotypes and alleles of β2-AR 27 loci among the three groups(p>0.05). Conclusion; The Gly16 polymorphism of β2-AR was overrepresented in nocturnal asthmatic patients, correlated with nocturnal asthma, and therefore appeared to be an important genetic factor in the expression of this asthmatic phenotype.