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罗廷荣, 源宣之, 金城俊夫
广西农业生物科学,1999,18(4):261~266,-0001,():
-1年11月30日
本试验利用杆状病毒载体成功地表达狂犬病病毒糖(G)蛋白。从转染重组了日本动物用狂犬病病毒疫苗株RCHL株G蛋白基因的转移载体和野生型杆状病毒DNA的Sf-9细胞中,分离出2个重组杆状病毒克隆。应用印渍法和荧光抗体法(IFA)从重组杆状病毒感染的Sf-9细胞检出了狂犬病毒G蛋白。Sf-9细胞表达的G蛋白与狂犬病毒RCHL株感染NA细胞的G蛋白的分子量一致。35个抗RCHL株G蛋白的单克隆抗体均与重组杆状病毒感染的Sf-9细胞反应,表明表达的G蛋白的抗原性没有发生变异。表达的G蛋白主要分布在Sf-9细胞的膜表面,形成类似环状的荧光,这种荧光与RCHL株感染的NA细胞的荧光相同。
狂犬病病毒, 糖蛋白, 重组杆状病毒
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罗廷荣, 李开鹏, 刘芳, 冯励, 潘艳, 莫全记, 李松, 罗永莉, 魏勇, 余克伦
广西农业生物科学,2005,24(4):287~290,298,-0001,():
-1年11月30日
从广西南宁、百色、柳州市采集了犬脑组织268份,从南宁市、博白县捕获蝙蝠320只,以及从南宁市郊捕获野鼠65只。应用RT-PCR技术对犬脑、蝙蝠和野鼠脑组织进行狂犬病病毒检测,并将阳性材料进行小白鼠脑内接种试验。检测结果表明,犬脑的狂犬病病毒阳性率为1.12%,蝙蝠阳性率为0.94%,野鼠阳性率为3.1%。本调查证明了广西除犬之外,野鼠和蝙蝠等野生动物也携带有狂犬病病毒。
反转录聚合酶链反应, 狂犬病病毒, 犬, 蝙蝠, 野鼠
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罗廷荣, It. Ito, N. Minamoto, H. Goto, T. R. Luo, M. Sugiyama, and T. Kinjo
Arch Virol (1996) 141: 2129-2138,-0001,():
-1年11月30日
The cDNA encoding the VP6 gene of avian rotavirus PO-13 strain was inserted into the bacterial expression vector pET-3a. Upon isopropyl-1-thio-13-D-galactoside induction, the E. coti BL21 (DE3) harboring the vector containing cDNA of the VP6 gene produced an approximately 45-kDa polypeptide, which reacted with rabbit serum against PO-13 strain in Western blotting. To study the antigenic sites on VP6, various deletion mutants were constructed, expressed in E. coli and the reactivity with antigenic site I- and Iispecific MAbs analyzed by Western blotting. Site I, which is shared with all group A mammalian and avian rotaviruses except for chicken rotavirus, was found to be located at amino acid positions 45 to 65, and site II, which probably contributes to an authentic group A antigen common to both mammalian and avian rotaviruses, at amino acid positions 134 to 142.
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罗廷荣, Ting Rong Luo, Nobuyuki Minamoto*, Hiroshi Ito, Hideo Goto, Shinya Hiraga, Naoto Ito, Makoto Sugiyama, Toshio Kinjo
Virus Research 51(1997)35-41,-0001,():
-1年11月30日
We have established a hybridoma producing monoclonal antibody (MAb) against a linear epitope of glycoprotein (G protein) of the RC-HL strain of rabies virus. This MAbl5-13 showed almost the same neutralizing activity to all of five rabies fixed strains, including RC-HL, and reacted to the denatured G protein in western blot analysis. To characterize and map this linear epitope, an antigenic variant NR15-13 was selected from RC-HL strain in the presence of neutralizing MAbl5-13. The variant reacted with MAbl5-13 in an immunofluorescent antibody test but was not neutralized by the antibody and the antibody did not bind to the variant G protein in a Western blot analysis. The variant NR15-13 had an amino acid substitution at position 251 of the G protein, where tryptophan of the parental RC-HL strain was replaced by arginine. Site-directed mutagenesis analysis using the expression system in simian COS7 cells revealed that a single amino acid substitution at 251-tryptophan by arginine on the G protein of the parental RC-HL strain abolished the antigenicity of the epitope for MAbl5-13 in western blot analysis, and the replacement of 251-arginine by tryptophan recovered the activity. These results strongly suggest that tryptophan at position 251 on the G protein is essential for construction of the linear epitope against MAbl5-13.
Rabies virus, Glycoprotein, Monoclonal antibody, Linear epitope
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