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【期刊论文】Microbial community analysis and performance of a phosphate-removing activated sludge
刘燕, Yan Liu, Tong Zhang, Herbert H.P. Fang *
Bioresource Technology 96(2005)1205-1214,-0001,():
-1年11月30日
The microbial community of a phosphate-removing activated sludge was analyzed according to the extracted 16S rDNA sequences. The sludge, which accumulated 5.6% P by weight, was obtained from a sequencing batch reactor treating a fatty-acid rich wastewater containing 108 mg l_1 total organic carbon (TOC), 14.0mg l_1 N and 16.2 mg l_1 P. The reactor at 25℃ and pH 7.6 removed over 96% TOC and 99.9% P from the wastewater. According to the 16S rDNA analysis of the 114 clones developed, the sludge had a diverse population, mainly comprising Proteobacteria (71.0%) and the Cytophaga Flavobacterium Bacteroides group (23.7%), plus a few species of Planctomycetales (2.6%), Verrucomicrobiales (1.8%) and Firmicutes (0.9%). Of the 114 clones, 36 (31.6%) were closely affiliated with Acinetobacter. However, Acinetobacter did not accumulate phosphate judging from the images of sludge samples hybridized with an Acinetobacter-specific probe and stained with a phosphate-specific dye. The identities of the phosphate-removing bacteria remain unclear.
Acinetobacter, DAPI, FISH, Phosphate, Wastewater, 16S rDNA
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刘燕, 李亮, 黄丽, 刘燕*
环境污染治理技术与设备,2004,5(12):1~6,-0001,():
-1年11月30日
回顾了国内外污水厌氧生物处理工艺的发展情况,着重分析了中国城市污水的特点以及厌氧生物处理工艺在城市生活污水处理中的应用情况。
低浓度, 低温, 厌氧处理, 城市污水
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刘燕, 曾次元, 李亮, 赵心越
复旦学报(自然科学版),2006,45(3):348~352,-0001,():
-1年11月30日
研究了电化学氧化法除氨氮(NH3-N)的影响因素。结果表明:电化学氧化过程中,氨氮去除速率与氨氮的初始浓度无关;随着电流密度的增大,氨氮去除速率和能耗增加;高Cl-浓度或中性条件有利于氨氮的去除。城市污水处理实验表明,电解4h后,初沉池出水处理后氨氮浓度从26.8降为6.1mg·L-1;二沉池出水处理后氨氮浓度从19.9降为0.1mg·L-1,优于I类地表水环境质量标准。
氨氮, 电化学氧化, 影响因素, 城市污水
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刘燕, Tong Zhang, Yan Liu, Herbert H.P. Fang
BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 2, OCTOBER 20, 2005,-0001,():
-1年11月30日
Anacetate-rich wastewater, containing 170mg/L of total organic carbon (TOC), 13 mg/L of N, and 15 mg/L of P, wastreated using the enhanced biological phosphate removal (EBPR) process operated in a sequencing batch reactor. A slight change of pH of the mixed liquor from 7.0 to 6.5 led to a complete loss of phosphate-removing capability and a drastic change of microbial populations. The process steadily removed 94% of TOC and 99.9% of P from the wastewater at pH 7.0, but only 93% TOC and 17% of P 14 days after the pH was lowered to pH 6.5. The sludge contained 8.8% P at pH 7.0, but only 1.9% at pH 6.5. Based on 16S rDNA analysis, 64.8% of the clones obtained from the sludge at pH 7.0 were absent in the pH 6.5 sludge. The missing microbes, some of which were likely responsible for the phosphate removal at pH 7.0, included b-Proteobacteria, Actinobacteria, Bacteriodetes/Chlorobi group, plus photosynthetic bacteria and Defluvicoccus of the a-Proteobacteria.Amongthem, the last two groups, which represented 9.3% and 10.1% of the EBPR sludge at pH 7.0, have rarely been reported in an EBPR system.
16S rDNA, EBPR, GAO, PAO, pH, phosphate removal
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【期刊论文】胞外聚合物对浸没式膜-生物反应器膜过滤性能的影响
刘燕, 王雪梅, 华志浩, 郭国良
环境科学学报,2005,25(12):1602~1607,-0001,():
-1年11月30日
通过浸没式膜-生物反应器(SMBR)处理上海市城市内河水的试验,研究了反应器混合液中和2种疏水性不同的膜上污泥胞外聚合物(EPS)的量、组成、污泥特性及对膜过滤性能的影响。结果表明,混合液污泥中的溶解性EPS和固着性EPS比膜上污泥多,不同污泥的固着性EPS组分中蛋白质(EPSP)P多糖(EPSC)的大小顺序依次为混合液污泥<亲水膜污泥<疏水膜污泥。疏水膜污泥中溶解性EPS比亲水膜多,溶解性EPS和固着性EPS量及组分均有很大差异。混合液污泥的EPS流动性大,疏水性膜上污泥EPS的流动性较大。溶解性EPS对污泥特性及膜过滤性能影响较大。SMBR中亲、疏水性膜片上黏附的污泥的相对疏水性分别为53%、59%。比较2种膜片通量的变化发现,疏水性膜污染严重。
浸没式膜-生物反应器, 胞外聚合物, 膜污染, 亲水性膜, 疏水性膜
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