The purpose of these experiments is to study the effects of Chinese herbal medicinal ingredients (CHMIs) on peripherallymphocyte proliferation and serum antibody titer in chicken vaccinated with Newcastle disease (ND) vaccine. Nine CHMIswere chosen for the experiments. Astragalus polysaccharide (APS), Isatis root polysaccharide (IRPS), Epimedium flavone(EF), Propolis flavone (PF), Astragalosides (AS) and Ginsenosides (GS) could promote lymphocyte proliferation andantibody titer, while Epimedium polysaccharide (EPS) mainly stimulated cellular immune responses. Chinese angelicapolysaccharide (CAPS) and Propolis polysaccharide (PPS) exerted weaker effects on promoting immune responses. APS,IRPS, PPS and PF in promoting lymphocyte proliferation, and IRPS, PPS, EF and PF in promoting humoral immunity inhigher dose were significantly stronger than in lower dose. Our results indicated that almost all of the nine CHMIs couldpromote both humoral and cellular immune responses and would be expected as the component drug of a new-typeimmunopotentiator.

In order to compare the adjuvant activity of compound Chinese herbal medicinal ingredients (cCHMIs) and filtrate best prescription anddose, two initial screened cCHMIs were prepared and mixed with Newcastle disease (ND) vaccine respectively at three doses to vaccinate17-day-old chickens, taking the non-adjuvant and oil adjuvant vaccines as controls. On day 7, 14, 21, 28, 35 and 42 after vaccination, thedynamic changes of peripheral lymphocyte proliferation and serum antibody titers in the chickens were determined by means of MTT methodand hemagglutination inhibition test. The results showed that two cCHMIs could promote peripheral lymphocyte proliferation and enhanceserum antibody titer, of which adjuvant activity was similar to and even stronger than oil adjuvant in promoting lymphocyte proliferation atsome time points, and there was a certain dose-effect relationship. The best adjuvant activity of cCHMIs 2 in promoting cellular immunityoccurred at middle dose, and cCHMIs 1 in enhancing humoral immunity, at high dose. Based on good synergistic effects of their components,two cCHMIs would be expected as new-type immunologic adjuvants to substitute for oil adjuvant.

用新城疫Ⅳ系疫苗免疫雏鸡，在免疫前、后分别注射高、低剂量的当归多糖、黄芪多糖、板蓝根多糖、淫羊藿多糖、蜂胶多糖、淫羊藿黄酮、蜂胶黄酮、黄芪皂甙和人参皂甙等9种中药成分，用β微量法监测血清中血凝抑制抗体效价的动态变化。结果表明，9种中药成分均能不同程度地提高抗体效价，且与给药时间、剂量和免疫接种次数有一定关系。

将安全浓度范围内的黄芪多糖、当归多糖、蜂胶多糖、淫羊藿多糖、板蓝根多糖、黄芪黄酮、蜂胶黄酮、淫羊藿黄酮、黄芪皂苷和人参皂苷等10种中药成分分别加入已培养24h、刚形成单层的鸡胚成纤维细胞（CEF）中，在加药后12，24，36，48 和60h用MTT法测定它们对CEF增殖的影响。结果表明，黄芪皂苷、黄芪多糖、板蓝根多糖和蜂胶黄酮主要表现为促进增殖; 淫羊藿多糖主要具抑制效应; 其他5种中药成分在某些浓度和时间点能促进增殖，而在某些浓度和时间点则抑制增殖，并有一定的量效和时效关系。

分别将5种浓度的黄芪多糖（APS）、当归多糖（CAPS）、淫羊藿多糖（EPs）、板蓝根多糖（IRPS）、蜂胶多糖（PPS）、黄芪黄酮（AF）、淫羊藿黄酮（EF）、蜂胶黄酮（PF）、黄芪皂甙（AS）和人参皂甙（GS）10种中药成分加入到培养24 h的鸡胚成纤维细胞（CEF）中，再培养12 h后接种新城疫病毒（NDV），于病毒接种后72h用中性红染料吸收法测定CEF活性以评价各中药成分对细胞增殖及其抵抗病毒感染的影响。结果表明，10种中药成分均能不同程度地促进细胞增殖和抵抗病毒感染，前者以AF、APs、IRPs、PF和Gs的作用较强，后者以Gs和IRPs的作用较强，部分中药成分在两方面作用中各有所长，且有一定的量效关系。