Microfluidics has been considered as a potential technology to miniaturize the conventional equipments and technologies. It offers advantages in terms of small volume, low cost, short reaction time and high-throughput. The applications in biology and medicine research and related areas are almost the most extensive and profound. With the appropriate scale that matches the scales of cells, microfluidics is well positioned to contribute significantly to cell biology. Cell culture, fusion and apoptosis were successfully performed in microfluidics. Microfluidics provides unique opportunities for rare circulating tumour cells isolation and detection from the blood of patients, which furthers the discovery of cancer stem cell biomarkers and expands the understanding of the biology of metastasis. Nucleic acid amplification in microfluidics has extended to single-molecule, high-throughput and integration treatment in one chip. DNA computer which is based on the computational model of DNA biochemical reaction will become practice from concept in the future. In addition, microfluidics offers a versatile platform for protein-protein interactions, protein crystallization and high-throughput screening. Although microfluidics is still in its infancy, its great potential has already been demonstrated and will provide novel solutions to the high-throughput applications.

We proposed a multi-frequency sandwich type vibration energy harvester to widen the effective frequency range of vibration energy harvester. The harvester is composed of three resonant structures with different natural frequencies. The resonant structures are two cantilevers each with bi-layer coils and a plane spring with a magnet. The maximum peak-peak voltages of the three different frequencies are 172 mV, 104 mV and 112 mV at the frequencies of 235 Hz, 330 Hz and 430 Hz, respectively. The first maximum voltage is much higher than the others, because the coils in both cantilevers can produce voltages.

Circulating tumor cells (CTCs) from peripheral blood are emerging as a useful tool for the detection of malignancy, monitoring disease progression, and measuring response to therapy. We describe a unique microfluidic chip that was capable of efficient and selective separation of CTCs from peripheral whole blood samples. The ability of microfluidic chip to capture CTCs from PBS and whole blood samples was tested.Sixty-eight peripheral blood samples from 68 colorectal cancer patients were investigated for the presence of CTCs by microchip technology. The frequency of CTCs was analyzed statistically for correlation with relevant clinical data. We also examined samples from 20 healthy individuals as controls. The calculated capture efficiency was 85.7% and decreased significantly at flow rates above 2.0 ml/h. The number of CTCs isolated ranged from 3 to 236/ml for colorectal patients [99 ± 64 (mean ± SD) CTCs/ml]. None of the 20 healthy subjects had any identifiable CTCs. We identified CTCs in 46 (67.65%) of the 68 patients: in two of nine (22.22%) Dukes A, in 10 of 24 (41.67%) Dukes B, in 21 of 22 (95.45%) Dukes C, and in all 13 Dukes D patients. The detection rate in Dukes C and D patients was much higher than in Dukes A and B patients (97.73% vs. 36.36%) (p < 0.01). A significant correlation between detection of CTCs and clinical stage (r = 0.792, p < 0.01) was found, which was higher than carcinoembryonic antigen (r = 0.285, p > 0.01), carbohydrate antigen 19-9 (r = 0.258, p > 0.01), α-fetoprotein (r = 0.096, p > 0.01), and cancer antigen 125 (r = 0.134, p > 0.01). Microfluidic chip provides a novel method for capturing CTCs. The presence of CTCs correlated with clinical stage. It is important to evaluate CK-positive and DAPI-stained tumor cells together to determine the role of CTCs in tumor behavior and disease progression.

癌症是影响人类健康的重大疾病之一, 如何快速、方便地分离癌细胞已经成为制约临床医学和科学研究的重要问题。本文将磁珠技术与MEMS技术相结合, 提出了一种等边三角排列的N i微磁柱结构, 在磁场作用下使用CD326免疫磁珠捕获结肠癌细胞。本文对MEMS细胞捕获芯片进行了设计、加工和封装, 采用该芯片对与磁珠结合的结肠癌细胞进行了捕获, 0. 1m l/h流速下捕获效率达92%,证明该芯片具有对结肠癌细胞的捕获能力。

设计了一种新型的脑电图干电极, 主要用于采集脑电信号以进行基于脑电图的警觉度分析, 替代传统的湿电极. 采用柔性衬底微电子机械系统( MEMS) 加工技术, 在柔性衬底上制备出具有化学稳定性与生物相容性的微针状干电极阵列. 通过铜牺牲层实现干电极微针的悬臂梁结构, 利用聚二甲基硅氧烷( PDMS) 剥离层实现器件从玻璃基底的完全释放, 并经平面电极的多层组装实现了立体电极阵列. 实验中干电极以聚酰亚胺作为柔性衬底, 其针端及导线部分材料为镍, 针端部分表面镀金. 采用Neuroscan 的脑电信号采集放大器对干电极性能进行了测试, 测得阻抗约为10k 8, 其时域和频域信号与传统湿电极基本一致. 所制备的干电极成品率高、尺寸小、屏蔽佳、装配简单、可靠性好、机械强度大, 符合快速、无痛的脑电信号采集要求。