目的 探讨细胞间粘附分子-1（intercellular adhesion molecule-1, ICAM-1）在内耳免疫反应中的作用。方法　采用钥孔 血蓝蛋白激发已全身致敏的26只大鼠内耳，诱发其内耳免疫反应，然后通过免疫组化技术检测内耳的ICAM21的表达。结果 内耳激发后6小时，螺旋轴静脉及其回流小静脉即有ICAM21表达，12小时内淋巴囊及其囊周区出现ICAM21表达，在24～48小时内耳各部位ICAM21表达达最高峰。72小时各种炎性细胞浸润到内耳的各个部位。随后ICAM21表达逐渐减弱，28天完全消失。结论 内耳免疫反应时，ICAM21在炎性细胞从循环系统进入内耳的过程中发挥着重要作用，调控ICAM21表达的细胞因子也可能还来自内淋巴囊以外的其他细胞。

Objectives/Hypothesis: It has been recognized that immunological mechanisms could be involved in the pathogenesis of different inner ear disorders, such as progressive sensorineural hearing loss, Meniere's disease, and even sudden deafness. The endolymphatic sac acts as an immune control organ for the inner ear and has been considered as an effector site of the mucosa-associated lymphoid tissue. The purpose of the study was to determine the potentially immunological relationship between endolymphatic sac and Waldeyer ring equivalent, one of the most important affector organs in mucosa-associated lymphoid tissue. Study Design: Animal model. Methods: Thirty-six rats were employed. Two animals were killed for histological observation of Waldeyer ring equivalent, and another 34 animals were randomly divided into experimental and control groups and received bilateral intranasal immunizations with keyhole limpet hemocyanin or Freund adjuvant, respectively. The ears of immunized animals and control animals were examined for keyhole limpet hemocyanin-positive memory cells and immunoglobulin G-positive plasma cells with the technique of immunohistochemical analysis. The histopathological changes and cell proliferation in those ears were also assessed. Results: There were paired and organized lymphoid tissues in the lateral wall of the first choana in the rat. Keyhole limpet hemocyanin–positive lymphocytes appeared within the endolymphatic sac at 3 days after the first anti-genetic stimulus of the Waldeyer ring equivalent. Endolymphatic hydrops in the cochlea, elevated amounts of immunocompetent cells, and increased activity of cell proliferation within the endolymphatic sac were also considered after four intranasal injections of keyhole limpet hemocyanin. Conclusion: Presumably, endolymphatic sac is supplied with immunocompetent cells from Waldeyer ring equivalent and has an ability of coreaction with Waldeyer ring equivalent. Key Words: Endolymphatic sac, mucosa-associated lymphoid tissue, proliferation.

Objective Toinve stigate the role of peripheral immunocompetent lymphocytes during the immunere sponse in the innerear1 Methods Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen of three groups of donor animals, i1e1, group A immunized intradermally with keyhole limpet hemocyanine (KLH) and both inner ears challenged with KLH, group B received only KLH intradermally without inner ear challenge, and group Cused as control, were isolated and labelled with 51 Cr1 The labelled lymphocytes were injected intravenously into the recipients1 They were immunized intradermally with right inner ears challenged with KLH1 Twenty to 24hlater, the radioactivities in the temporal bones of the recipients were assayed to detect the infiltration of labelled cells1

To explore whetber The immune response of the inner ear could induce heat shock protein 70 (hsp70) in tile guinea pig cochlea, we established a model of autoimmune inner eat" disease (AIED) by systemically immunizing guinea pigs with homologous crude inner ear antigen (CIEAg).The immunized cochleas and normal control cochleas were examined for the expression of hsp70 by immunohistochemical analysis and in situ hybridization. It was found that 10 of 28 ears showed significant increases in hearing thresholds after the animals had been sensitized with CIEAg, and 67.9% of the ears from the immunized animals had modiolar vasculitis. The hsp70-1ike proteins were basically expressed in the normal cochleas, but dramatically increased expressions of hsp70 (perhaps also including hsp70-1ike molecules) and its messenger RNA were observed in the cochleas of the AIED models. The restltS suggest that the immune response of the inner ear induced the expression of hsp70 in The guinea pig cochleas.

Objective To explore whether hypertension can accelerater aging process of the hearing organ, and to understand the relationship between hypertension and aging process. Methods Three-month-old and 12-month-old Wister rats (each group 20), and 3-month-old and 12-month-old SHRSP ratsf (each group 20), with normal tyrnpanic membreane, weighing 200-300g (3-month-old) and 40-600g (12-month-old) (Institute of Cardiovascular Disease, Chinese Academy of Medical Sciences), were selected as two control groups and two experimental groups respectively. The tail artery blood pressure was measured without injury. The threshold of auditory brainstem response (ABR) WAS MEASURED WITH spirit TM evoked potential meter (Nicolet Company)l. The enzyme histochemistry in the inner ear (LDH and ChE) was examined and their optical density were analyzed with HPLAS analytical system. Results It showed no difference in ABR thresholds and activites of LDH and ChE between 3-month-old Wister and SHRSP rats (P>0.05). The mean value of ABR threshold of 12-month-old Wister rats was higher than that of the two 3-month-old groups (P<0.05), LDH and ChE activity of the 12-month-old Wister rats at relevant section were significantly higher and lower respectively than those of the two 3-month-old group (p<0.05), whereas the mean value of ABR threshold of the 12-month-old SHRSP rats and the activities of LDH and ChE at relevant section were significantly higher than those of the wto 3-month-old groups (P<0.01). Conclusions It suggestes that presbycusis exists in 12-month-old Wister rats, and glycogenosis and abnormal secretory of neural transmitter was discerned after hypertension. All above factors may worsen the aging of the hearing system.