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【期刊论文】Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents
李保新, Baoxin Li, Zhujun Zhang*, Yan Jin
Sensors and Actuators B 72 (2001) 115-119,-0001,():
-1年11月30日
A novel chemiluminescence (CL) flow biosensor for hydrogen peroxide is described. It is prepared by immobilizing HRP with the sol-gel method on the flow CL cell, and by electrostatically immobilizing luminol on a strongly basic anion-exchanger resin. Hydrogen peroxide is sensed by the CL reaction with HRP sensitive membrane and luminol bleeding from the ion-exchanger column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 1
Chemiluminescence, Flow biosensor, Sol-gel method, Hydrogen peroxide
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李保新, Baoxin Li, Zhujun Zhang*, Yan Jin
Biosensors and Bioelectronics 17 (2002) 585-589,-0001,():
-1年11月30日
A novel plant tissue-based chemiluminescence (CL) biosensor for dopamine combined with flow injection analysis is presented in this paper. The potato roots act as molecular recognition elements. Dopamine is oxidized by oxygen under the catalysis of polyphenol oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of potato tissue to generate CL signal. The CL emission intensity was linear with dopamine concentration in the range of 1×10-5-1×10-7g/ml and the detection limit was 5.3×10-8g/ml (3σ) with a relative standard deviation of 1.7%. Combined with microdialysis sampling, the biosensor was applied to monitor the variation of dopamine level in the blood of rabbit after the administration of dopamine to demonstrate the favorable resolution and reliability of the system for in vivo on-line monitoring.
Chemiluminescence, Plant tissue-based biosensor, Flow injection, Microdialysis, Dopamine
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【期刊论文】Plant Tissue-Based Chemiluminescence Flow Biosensor for Glycolic Acid
李保新, Baoxin Li, Zhujun Zhang, * and Yan Jin
,-0001,():
-1年11月30日
A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic acid combined with flow injection analysis is proposed in this paper. The spinach tissue acts as the molecular recognition element. Glycolic acid is oxidized by oxygen under the catalysis of glycolate oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4
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