A novel chemiluminescence (CL) flow system for the determination of sulfite is described. It is based on the direct CL reaction of sulfite and silver(Ⅱ) in acid media without any sensitizers. The unstable Ag(Ⅱ) is on-line electrogenerated via a positive Pt-electrode from AgNO3 in HNO3 medium by constant current electrolysis, resulting in the elimination of the inconvenience of its instability to water. The CL intensity emission intensity was linear with sulfite concentration in the range 4×10−7-1×10−5mol/l; the detection limit was 1.7×10−7mol/l (3). The system was applied satisfactorily to the determination of sulfite dioxide in air.

A novel chemiluminescence (CL) flow system for the determination of quinine is described. It is based on the direct chemiluminescence reaction of quinine and cobalt(Ⅲ) in sulfuric acid medium. The unstable Co(Ⅲ) was on-line electrogenerated by constant-current electrolysis. The chemiluminescence intensity was linear with a quinine concentration in the range of 0.1-100μg ml-1. The determination limit was 3.3×10-8g ml-1. The whole process could be completed in 1min. The proposed method is suitable for automatic and continuous analysis, and has been applied successfully to the analysis of quinine in pharmaceutical preparation.

The novel flow chemiluminescence (CL) system for determination of pipemidic acid was proposed, which was based on the sensitizing effect of pipemidic acid on the CL oxidation of sulfite by KMnO4 in acid media. Combined with the technique of ultrafiltration, the flow-injection CL system was applied to study in vitro the bovine serum album (BSA) binding of pipemidic acid. The estimated association constant (K) and the number of the binding site (n) on one molecule of BSA were 8.81

A novel chemiluminscence (CL) flow-through sensor for pipemidic acid is described. It was based on the sensitizing effect of pipemidic acid on the CL oxidation of sulfite by sodium bismuthate in H2SO4 media. The solid-phase sodium bismuthate was mechanicially immobilized on the sponge rubber inside of the CL flow cell as CL oxidant. The calibration graph is linear in the range 0.1-10g/ml with a detection limit of 6.2×10−8g/ml (3σ). A complete analysis couldbe performedin 1 min with a relative standard deviation (R.S.D.) of 2.5% for 2g/ml pipemidic acid (n=8). This methodhas been successfully applied to determine pipemidic acid in pharmaceutical preparation.

A novel chemiluminescence (CL) flow-through sensor for hydrogen peroxide is described. It is prepared by immobilizing hemoglobin (Hb) with the sol-gel method on the flow cell, and by electrostatically immobilizing luminol on strongly basic anion-exchange resin. Hydrogen peroxide is sensed by the CL reaction with Hb sensitive membrane and luminol bleeding from the ion-exchange column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 6×10−5 to 4×10−7 mol l−1 with a detection limit of 1.3×10−7 mol l−1 (3σ). A complete analysis could be performed in 1 min with a relative standard deviation of 1.7% for 5×10−6mol l−1 H2O2 (n=9). The sensor has been applied successfully to the determination of hydrogen peroxide in rainwater.