Frequencies of echolocation calls with maximum power of Himalayan leaf-nosed bats and Horsfield's leaf-nosed bats during searching phase were 74.1 and 92.1kHz, respectively. Head-body length, forearm length and body mass of Himalayan leaf-nosed bats were 82.9mm, 89.7mm and 59.1g, respectively; and the corresponding values of Horsfield's leaf-nosed bats were 68.4mm, 61.3mm and 19.7g, respectively. Echolocation frequency and the three parameters of body size, head-body length, forearm length and body mass, were all negatively correlated, and the correlation coefficients were -0.86, -1.58 and 2.19, respectively. This study thereby proved that echolocation frequency and body size were negatively correlated in the two species of hipposiderid bats.

Phylogenetic relations among five species of Hipposideridae and seven species of Rhinolophidae including one endemic species (Rhinolophus rex) were examined by partially sequencing of the mitochondrial cytochrome b gene (528 bp). Analyses of the cytochrome b sequences of Hipposideridae and Rhinolophidae suggest that each formed a monophyletic group. All phylogenetic analyses indicate that Aselliscus should remain as a genus within Hipposideridae, with the mean percentage sequence differences (16.43%) and transition: transversion ratios (2.032) between Aselliscus and Hipposideros. Within Hipposideros, H. armiger shows close affinity to H. larvatus although it possesses superficial similarity morphological characters to H. pratti. Genetic distance values suggest that H. larvatus and H. armiger diverged from each other approximately 1.7-4.3 million years ago, and H. pratti diverged from the larvatus-armiger clade approximately 2.1-5.2 million years ago.

Echolocation calls of Myoti$ frater emitted during the search phase consist of brief frequency-modulated pulses. The sound signals are comprised of three harmonics of which the first one is of the highest intensity and occurs in all echolocation calls. The frequency of this fundamental harmonic ranges from 110.8 to 50.2kHz, and its duration is about 3.5ms. The second and the third harmonics are relatively weak, occurring in 50.0% and 25.0% of echolocation calls respectively, with frequencies ranging from 138.4 to 116.6 kHz for the second harmonic and from 193.6 to 170.8kHz for the third harmonic. We presume that, according to the echolocation call features, Myotis frater probably forages the insects on the ground of complex environments.

The correlation between echolocation sound and morphological features of 18 species of bats, which are subordinated to 3 families including rhinolophidae, hipposideridae and vespertilionidae is studied. Pearson's correlation and regression analysis are adopted to analyze the correlations between noseleaf width (NW) and forearm length (FAL), NW and dominant frequency (DF), FAL and DF. The results show a positive correlation between NW and FAL and a negative correlation between NW and DF in Rhinolophidae. In each family, there is a negative correlation between FAL and DF. Some species have a higher or lower frequency than predicted one according to their body size. There are significant differences of the regression equation of FAL and DF among the three families. For the bats with the same body size, the order in frequency is: hipposiderid bats> rhinolophid bats)" vespertilionid bats. The mechanism of interrelation-ship between FAL and DF of bats, the reason why these deviations occur in correlativity of the three families and the mechanism of the in- teraction between morphological, physiological and ecological characteristics are discussed.