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鞠熀先, Y.K. Ye a, J.H. Zhao b, F. Yan b, Y.L. Zhu a, H.X. Ju a, *
Biosensors and Bioelectronics 18(2003)1501-1508,-0001,():
-1年11月30日
Sequence-known short-stranded hepatitis B virus (HBV) DNA fragment (181 bps) was obtained by PCR method. The strategy for its electrochemical detection was designed by covalently immobilizing single-stranded HBV DNA on gold electrode surface via carboxylate ester as a linkage between 3'-hydroxy end of DNA and carboxyl group of thioglycolic acid (TGA) self-assembled monolayer. The hybridization reaction on surface was evidenced by electrochemical methods using ferrocenium hexafluorophosphate (FcPF6) as an electroactive indicator. The interactions of Fc+with single-stranded (ss) and double-stranded (ds) HBV DNA immobilized on TGA monolayer were studied. The difference between the responses of Fc+at ss-and ds-DNA/Au electrodes suggested that this hybridization biosensor could be conveniently used to monitor DNA hybridization with a high sensitivity. AC impedance and XPS techniques have been employed to characterize the immobilization of ss-DNA on the gold surface.
Hepatitis B virus, PCR, Self-assembled monolayer, Biosensors, Ferrocenium hexafluorophosphate
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鞠熀先, Dan Du a, Feng Yan b, Shengli Liu c, Huangxian Ju a, *
Journal of Immunological Methods 283(2003)67-75,-0001,():
-1年11月30日
We describe a novel electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) based on the immobilization of CA19-9 with titania sol-gel on a graphite electrode (GE) by vapor deposition. The CA19-9 membrane was characterized using scanning electron microscopy and proved to be chemically clean, porous and homogeneous. The incubation of the immunosensor in a solution containing horseradish peroxidase (HRP)-labeled CA19-9 antibody led to the binding of HRP-labeled antibody with the immobilized antigen. The immobilized HRP catalyzed the oxidation of catechol by H2O2 and this provided a competitive method for the measurement of serum CA19-9. The response current decreased with increasing CA19-9 concentration in the incubation solution. The effects of pH, amount of HRP-labeled antibody, incubation time and temperature were explored to provide optimum analytical performance. Under optimal conditions, the current decrease of the immunosensor was proportional to CA19-9 concentrations in the range of 3-20 U/ml with a detection limit of 2.68 U/ml at a current decrease of 10%. The detection of CA19-9 in two serum samples obtained from clinically diagnosed patients with pancreatic carcinoma showed acceptable accuracy. The proposed immunosensor provides a new promising tool for the clinical immunoassay of CA19-9.
Immunosensor, Electrochemical sensors, Biosensors, Enzyme-labeled antibody, Carbohydrate antigen 19-9, CA19-9, Titania sol-gel, Pancreatic carcinoma
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鞠熀先, Zong Dai, †, Feng Yan, Jin Chen, † and Huangxian Ju*
Anal. Chem. 2003, 75, 5429-5434,-0001,():
-1年11月30日
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鞠熀先, Songqin Liu and Huangxian Ju*
Analyst, 2003, 128, 3-7,-0001,():
-1年11月30日
0.1 to 9.7 μmol dm-3 and a detection limit of 0.06 μmol dm-3 at 3σ. The amperometric determination of high concentrations of NO2-based on the irreversible reduction of NO could be performed at pH 4.0 with a linear range from 0.1 to 1.2 mmol dm-3. The surface of biosensor could be renewed quickly and reproducibly by a simple polish step. The biosensor has been used satisfactorily for nitrite determination in native water samples.
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鞠熀先, Jiuhong Yu, Songqin Liu, Huangxian Ju*
Biosensors and Bioelectronics 19(2003)401-409,-0001,():
-1年11月30日
A novel amperometric glucose sensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared with a vapor deposition method. The sol-gel film was uniform, porous and showed a very low mass transport barrier and a regular dense distribution of GOD. Titania sol-gel matrix retained the native structure and activity of entrapped enzyme and prevented the cracking of conventional sol-gel glasses and the leaking of enzyme out of the film. With ferrocenium as a mediator the glucose sensor exhibited a fast response, a wide linear range from 0.07 to 15mM. It showed a good accuracy and high sensitivity as 7.2 μAcm-2 mM-1. The general interferences coexisted in blood except ascorbic acid did not affect glucose determination, and coating Nafion film on the sol-gel film could eliminate the interference from ascorbic acid. The serum glucose determination results obtained with a flow injection analysis (FIA) system showed an acceptable accuracy, a good reproducibility and stability and indicated the sensor could be used in FIA determination of glucose. The vapor deposition method could fabricate glucose sensor in batches with a very small amount of enzyme.
Biosensors, Electrocatalysis, Sol-gel, Titania, Vapor deposition, Glucose, Glucose oxidase, Flow injection analysis
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