Nine hundred and seventy-six bacterial isolates were obtained in this study, among which a bacterial antagonistic isolate, Bacillus subtilis S9 from sugarcane rhizosphere showed cell-lytic effect on the plant pathogens, Rhizoctonia solani, Pythium ultimum and Fusarium oxysporum f. sp. niveum after 4-day-confronting incubation on PDA plates. However, S9 could not cause obvious inhibitory zones on PDA plates during this incubation. Lytic spots of hyphae of R. solani caused by S9 were clearly presented under scanning electronic microscope. The cell-lytic process of S9 is as follows: Bacterial cells of S9 absorb on hypae of R. solani firstly, and grow and reproduce as the fungal hyphae grow, and then secrete some cell-lytic substances to decompose the hyphae. However, this study revealed that S9 hardly interfered with the growth of the fungal antagonists, such as Trichoderma viride, Chaetomium cupreum and Chaetomium globosum. Pot experiments proved that S9 could effectively control root disease of tomato caused by R. solani. It Suggested that S9 mixed with above fungal antagonists could potentially synergistically control plant diseases caused by pathogenic fungi.

MGTAl. A putative fungal Zn (Ⅱ)2CyS6 transcriptional activator-encoding gene, was isolated from rice blast pathogen Magnaporthe grisea. Which is homologous to CLTAI from Colletotrichum lindemuthianum with 51% identity at protein level. MGTAI cassette contains a 2370 bp open reading frame, consisting of 6 exons, coding a 790 amino acid peptide. MGTAI gene exists as a single copy in genomes of 7 stranins of M. grisea, and is expressed in tip hyphae, conidia and mature appressoria of strain Guyl 1.

A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λ TriplEx2 vector by SMARTTM cDNA library containing 2.37×10.6 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660bp. Of 9 randomly selected clones, 2 expressed tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the carly stages of penctration of M. grisea.