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A total of 985 bacterial isolates were obtained from rice leaves and other grasses. The isolates were cultared in nutrient broth (NB) medium. After Centrifugated, 8000r/min for 10min, the supernatants of each bacterial NB cultures were tested for their suppressive effects on the germination and appressorium formation of Magnaporthe grisea. Of the tested isolates, 13 isolates showed suppressive effects. Three isolates identified as Psendomonas fluorescens (Xh216), Bacillus sp. (Xh222) and Pseudomonas. Sp. (Xh240), suppressed the disease severities on the detached rice leaves with the disease severity reduction 72.3%, 70.5% and 67.4%, respectively.

Temperature sensitive mutants (ts-mutants) are godd material for studying cell division and differentiation. Two hundred and seventy-four ts-mutants of Magnaporthe grisea were induced by UV radiation. These mutants could grow at 25℃, but not at 33℃. Pathogenicity test indicated that the pathogenicity of 32 ts-mutants reduced distinctly. Under 33℃ conditions, seven of them did not germinate, 12 stopped growing after germination and no appressorium formed, nine stopped growing after appressorium formed, for mutants formed swell and bunch appressorium, then stopped growing. The ability of perithecia formation of some mutants was changed.