The roots of several Pulsatilla species (Ranunculaceae) are used for "blood-cooling" and detoxifying effects in traditional Chinese medicine.1 A number of Pulsatilla species, such as P. ambigua, P. chinensis, P. dahurica, P. koreana, and P. turczaninovii, are employed in treating diarrhea, vaginal trichromoniasis, and bacterial infections, and pharmacological investigations have suggested that the triterpene saponins are important bioactive components. 2-6 We have previously reported on the isolation and structural elucidation of new triterpene glycosides such as pulsatillosides A and B from P. chinensis 5 and patensin from P. patens var. multifida.6 Further investigation of the latter plant has now led to the purification of additional saponin constituents. In this paper we describe the isolation of seven saponins, the structures of which were determined with the aid of 1D and 2D NMR (13C, 1H, HMQC, HMBC, and ROESY) techniques, FABMS, and hydrolysis. An extensive search of the literature 7-11 indicated thatcompounds 1-5 are new structures.