The aim of this study was to investigate the effects of a post-weaning isocaloric hyper-soybean oil diet on later obesity and explore the underlying mechanisms. In the present study, newborn male Wistar rats were weaned on d 24, divided into CON (control), HC and HSO groups. CON was assigned to AIN-93G diet (a hypercarbohydrate diet, for short HC diet) during the entire experiment. HC and HSO were fed with HC and isocaloric hyper-soybean oil (HSO) diet for 3 wk respectively, fed with HC diet for 2 wk successively, finally administrated high fat diet (HF) for 6 wk to induce obesity. On 3,5,11wk, the body weight, body fat content, blood glucose, blood lipid, serum insulin and leptin levels and obesity-related gene (CPT-1, FAS, UCP2, UCP3) expression levels in rats were detected. It was shown that body weight, body fat content, blood glucose and blood lipid, serum insulin and leptin levels in HSO were down-regulated on 3 and 5wk, therefore were significantly reduced on 11wk vs. HC. The CPT-1, UCP2, UCP3 gene expressions were up-regulated but FAS were down-regulated persistently in HSO. The study indicated that an early isocaloric HSO diet may reduce later obesity risk and reduce blood lipid and glucose abnormalities in adulthood via persistently influencing insulin and leptin sensitivity and permanent regulation of obesity-related gene expressions.

目的 了解哈尔滨市居民膳食钙摄入情况以及机体钙营养状况。方法　在哈尔滨市选择代表性社区，以512名健康成年人为调查对象(平均40岁)，分别于2004年和2005年采用食物频率问卷法进行膳食问卷调查，同时采集调查对象血样，测定钙营养相关指标以及骨密度。结果　哈尔滨市居民夏秋季人均膳食钙摄入量为383mg/d，冬春季人均膳食钙摄入量为479mg/d，年平均人均膳食钙摄入量为431mg/d。钙营养相关指标，血清钙、血清磷、血清碱性磷酸酶、降钙素以及1,25(OH)2D3均在正常值范围内，甲状旁腺激素高出正常范围，25(OH)D均值低于正常值范围。逐步多元回归结果显示，膳食钙摄入量与能量摄入的比值和蛋白质摄入量与骨密度Z评分有关，前者呈正相关，后者呈负相关。结论　哈尔滨市居民膳食钙摄入水平远未达到钙的DRI，机体钙营养不充足。补钙的同时应注意能量的摄入不要过高，以能够更有效地补钙。

目的　探讨解偶联蛋白在大鼠抵抗饮食诱导肥胖中的作用。方法　将50只健康雄性SD大鼠随机分为对照组和高脂组，分别用基础饲料和高脂饲料喂养13周，然后根据体重和能量摄入量筛选出饮食诱导肥胖抵抗(diet-induced obesity resistance，DIO-R)和饮食诱导肥胖(diet-induced obesity，DIO)组，观察体重的变化；RT-PCR 法测定大鼠褐色脂肪、白色脂肪及骨骼肌中解偶联蛋白mRNA水平。结果　DIO-R 大鼠体重明显低于DIO 大鼠(P<0105)；高脂饲料可增加DIO-R大鼠褐色脂肪解联偶蛋白(UCP)mRNA、白色脂肪UCP2 mRNA 及骨骼肌UCP3 mRNA 水平。结论　解偶联蛋白表达增加在肥胖抵抗大鼠的能量消耗中起重要作用，是大鼠抵抗肥胖发生的部分原因。