朊病毒病是由一种新型蛋白感染因子引起的人和动物可转移神经退化性疾病， 又称海绵状脑病， 最终可导致患者死亡。作者就朊病毒的致病机理， 从朊病毒学说， 致病性、生物学特性进行阐述， 并从病原基因遗传结构特点， 致病蛋白的结构特点和形成机制作以综述， 为该类疾病的防治提供理论依据。

根据已报道的哺乳动物朊病毒基因序列设计引物对，采用PCR方法扩增了大熊猫的朊病毒基因，将其克隆到T-Easy 载体，序列测定及分析表明所克隆的大熊猫PRNP基因（GeneBank 收录号为AF327449）片段为795bp，编码264个氨基酸的前体蛋白，推测其分子量约28.5ku。与已报道的牛（GeneBank 收录号为AF455119）、绵羊（GeneBank 收录号为AF367623）的相应序列作比较分析，核苷酸序列同源性分别为99%和83%，其编码的氨基酸同源性均为100%。在所克隆的大熊猫PRNP基因中未发现与朊病毒敏感性连锁的氨基酸多态性位点。

Determination of tissue-specific expression of cellular prion protein (prpc) is essential for understanding its poorly explained role in organisms. Herein we report on quantification of prp Mrna in golden hamsters,a popular experimental model for studying mechanisms of transmissible spongiform encephalopahies (TSE), by real-time RT-PCR.Total RNA was isolated from four different regions of the brain and six peripheral organs of eight golden hamsters Prp Mrna copy number were determined using absolute standard curve method with real-time quantitative PCR instrument.I was found that high Mrna levels were present in all four regions of the brain examined,ingunal lymph node showed high level of the expression similar to that in overall brain; spleen, heart, liver, and lung showed moder-ate levels of the expression; and kidney showed the lowest expression. Our result is consist-ent with the potential involveoment of different organs in prion diseases and offers essentialdata for further study of TSE mechanism in this animalmodel.

The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9h and 48h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

以朊蛋白单抗AH6和碱性磷酸酶标记的酶标马抗鼠二抗建立了疯牛病和羊痒病的Western Blot检测方法。对Western Blot各种反应条件进行摸索，并确定了最佳工作条件，结果表明：当匀浆缓冲液为RIPA时最佳反应条件为浓缩胶内电泳电压为恒压90V，分离胶电泳电压为恒压160V，转印的最佳电压和时间为恒压100V 1.5小时；封闭液为3%BSA时，封闭15分钟，封闭效果最好；AH6的最佳稀释浓度为1：4000，4℃下孵育过夜，马抗鼠二抗的最佳稀释浓度1：1000，室温下孵育30 分钟。采用已确立的反应条件对样品进行检测并与Prionics®-Check WESTERN进口试剂盒的检测结果比较，发现其敏感性为100%，特异性为99.4%，而进口试剂盒分别为100%，100%，与进口试剂盒无显著差异，这为在该基础上建立国产试剂盒提供了条件