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李春, Chun Li, Ying-Jin Yuan*, Jin-Chuan Wu & Zong-Ding Hu
Biotechnology Letters 25: 1335~1343, 2003.,-0001,():
-1年11月30日
A structured kinetic model was established to describe the process of Taxol formation in suspension cultures of Taxus chinesis var. mairei induced by an oligosaccharide from Fusarium oxysporum. In this model, the role of intracellular starch as a storage carbon source had to be taken into account. Substrate uptake, culture growth, cell respiration, and secondary metabolites, predicted by the model, agreed with those obtained experimentally. The effective factors of oligosaccharide elicitation, ηe,j, defined as the ratio of the parameter values in the system with oligosaccharide to those in control, reflected the effects of the oligosaccharide on cell growth and Taxol production.
oligosaccharide,, plant cell culture,, structured kinetic model,, Taxol,, Taxus chinesis var., mairei
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李春, Ying-Jin Yuan*, Chun Li, Jin-Chuan Wu & Zong-Ding Hu
Biotechnology Letters 24: 407~412, 2002.,-0001,():
-1年11月30日
A non-linear cascade model is proposed to describe the signal transduction pathway in suspension cultures of Taxus chinensis var. mairei induced by an oligosaccharide from Fusarium oxysporum. The oxidative burst intensity, which was defined as the amount of the free radicals including superoxide anion (O2·), H2O2 and OH− and measured by ESR spectrometry, was used as the signal characteristic and the theory of electronic signal transduction cascade was then applied to derive the initial model. The model shows that the signal transduction cascade process is composed of three cascade nets, represented by the phosphorylation of a G-protein, activation of an ion channel and phospholipase C, and phosphorylation of protein kinase C. The three reactions were calculated as beginning at 30.5, 48.8 and 141.4min after elicitation, and the maximum variation rates of the signal intensity in the three nets occurred at 43.2, 80.6 and 192.6min, respectively. The validity of the model predictions was verified by experimental data.
oligosaccharide,, signal transduction,, suspension culture,, Taxol,, Taxus chinensis var., mairei
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李春, Ying-Jin Yuan*, Chun Li, Zong-Ding Hu, Jin-Chuan Wu
Enzyme and Microbial Technology 30(2002)774~778,-0001,():
-1年11月30日
The effects of oligosaccharide from Fusarium oxysprum on secondary metabolisms in suspension cultures of Taxus chinensis var. mairei were investigated by detecting the oxidative burst (OB) signals using an Electron Paramagnetic Resonance (ESR) spectrometer. The oxidative burst signals induced by oligosaccharide gradually increased to a maximum at 4h. Phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) activities were respectively 2 and 4 times higher than those of control, and large amount of phenolics were accumulated in culture medium. Two times of obvious increase in taxol production was observed, which was in accordance with the two times of elicitation of oxidative burst signals. The dependence of taxol production on the intensity of OB signals obeyed a modified Logistic curve. It was inferred that the syntheses of the side chain and nucleus of taxol were enhanced at low and high intensities of OB signals, respectively.
Oligosaccharide, Fungus, Oxidative burst, Taxus chinensis var., mairei, Taxol, Suspension culture
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