A structured kinetic model was established to describe the process of Taxol formation in suspension cultures of Taxus chinesis var. mairei induced by an oligosaccharide from Fusarium oxysporum. In this model, the role of intracellular starch as a storage carbon source had to be taken into account. Substrate uptake, culture growth, cell respiration, and secondary metabolites, predicted by the model, agreed with those obtained experimentally. The effective factors of oligosaccharide elicitation, ηe,j, defined as the ratio of the parameter values in the system with oligosaccharide to those in control, reflected the effects of the oligosaccharide on cell growth and Taxol production.

Signal transduction pathway for oxidative burst and taxol production was studied in suspension cultures of Taxus chinensis var. mairei induced by oligosaccharide from Fusarium oxysprum. Suspension cells exhibited an oxidative burst approximately 4h after challenge with oligosaccharide. The secondary metabolism including accumulation of extracellular phenolics and taxol production were enhanced, while the alkalization of the outer medium and activity of phenylalanine ammonia lyase (PAL) was promoted significantly. Pretreatment with the tyrosine (Tyr) kinase inhibitor herbimycin A abolished the oxidative burst and the subsequent induction of taxol production within 15 min after addition of elicitor. Suramin, a G-protein inhibitor, inhibited the oxidative burst if added up to 30 min after oligosaccharide treatment. The oxidative burst was also induced by the ion channel generator amphotericin B. In contrast, the ion channel blocker anthracene-9-carboxylate acid and Ca2 channel blocker nifedipine inhibited the oxidative burst within 45min after treatment. Oligosaccharide rapidly induced Tyr phosphrylation of receptor, which was inhibited by herbimycin A and anthracene-9-carboxylate acid. These responses were also inhibited by phospholipase C-neomycin. The response time of oligosaccharide to phospholipase C pathway was ca at 60 min. These data suggested that the activation of ion channels and phospholipase C follow Tyr kinase and G-protein in the signal pathway leading to the oxidative burst.

利用热带假丝酵母（Candida tropicalis）突变株SP-UV-56进行十三碳二元酸发酵，研究了pH值控制模式对产酸的影响。低pH值对维持菌体活性有利，但不利于菌体产酸；高pH值能促进菌体产酸，但菌体活性有所下降。在研究中定义了3个参数pHe值、Cp，e和ΔpHe。菌体的产酸与ΔpHe有关，通过实验建立了pHe值与产酸量Cp,e之间的关联式，提出了pH值梯度控制策略，并确定了适宜的ΔpHe。以ΔpHe=012为推动力，建立了pH值连续控制方程。应用pH值连续控制方程进行发酵，转化120h产酸量达到145.2g·L-1，较对照组的产酸量提高了16%，适宜的pH值控制范围为715～812，为长链二元酸发酵技术实现pH值连续自动控制奠定了理论基础。

运用顺磁共振、电镜、能谱等手段研究了寡聚糖对红豆杉细胞氧化还原生。理态势的影响。结果表明，诱导前红豆杉细胞处于相对稳定的生长态势，细胞完整，细胞器发达；氧化还原电势较低，活性氧相关酶系统相对稳定, 细胞初生代谢旺盛，紫杉醇合成速率很低。诱导后红豆杉细胞向产物合成态势转移，SOD酶活性迅速提高，CAT和POD酶活被强烈抑制；细胞出现活性氧迸发，4h达最大值；细胞的氧化还原电势增高，细胞膜上离子通道被激活，H+和Ca2+ 内流，Cl-外流；液胞内出现大量含Ca2+离子的高电子致密区并呈规律性变化，培养环境碱化；细胞生长被抑制，紫杉醇合成速率升高，其产量提高了515倍，达7611mg·L-1。

Separation of l-glutamine (l-Gln) from Gln fermentation broth by nanofiltration (NF) was investigated with NTR7450 NF membrane. The effects of various experimental parameters such as transmembrane pressure, pH and concentration of broth on the rejection of l-Gln and l-glutamate (l-Glu) showed that NTR7450 was able to effectively separate l-Gln and l-Glu when the appropriate conditions were chosen. Moreover, the rejection of l-Gln and l-Glu in a single amino acid solution was also studied with the variation of pH, concentration, and physicochemical parameters. It was demonstrated that deviations in the rejection of fermentation broth from a single l-Gln or l-Glu solution were mainly caused by the complex ionic composition of the real fermentation broth.