The most important non-starch polysaccharides in barley grain are b-glucan and arabinoxylans. Arabinoxylans are partially water-extractable, high-molecular-weight polymers that contribute to viscosity and membrane filterability. Changes to arabinoxylans content and endo-xylanase activity during germination and kilning were studied. This paper was aimed at etermining the composition of total arabinoxylans and water-extractable arabinoxylans in malt and observing their changes during brewing. The water-extractable and total arabinoxylans contents of six barley malts and wheat malt varied in the range of 0.42-0.98% and 3.84-6.90%, respectively. Their L-arabinose-to-D-xylose ratio ranged between 0.49-0.59 (total arabinoxylans) and 0.60-0.85 (water-extractable arabinoxylans). Contents of arabinoxylans in worts mainly originated from the soluble part of arabinoxylans in malt. The use of rice adjunct decreased arabinoxylans contents of wort and beer. The correlation between arabinoxylans and viscosity was investigated. Results showed that water-extractable arabinoxylans in wort have positive and higher correlation value with wort viscosity than those of b-glucan in wort, and both water-extractable arabinoxylans and b-glucan in beer have significant correlation (p<0.01) with beer viscosity.

The inhibitory effects of 1,3-diacylglycerol (DAG) on diet-induced lipid accumulation in liver and abdominal adipose tissue of rats were investigated in the present study. Male Sprague-Dawley rats were given free access to diets containing 7 wt% TAG (low TAG), 20 wt% TAG (high TAG), or 20 wt% DAG (high DAG), respectively, for 8 wk. The body weight of rats in the 20% high-TAG group increased significantly, and the weights of their abdominal adipose tissue and liver also showed a significant increase compared with rats in the low-TAG group. However, the high-DAG diet resulted in both a significant reduction in body weight gain (with a decrease of 70.5%) and an increase in the ratio of abdominal fat to body weight (by 127%) compared with the high-TAG diet. As well, the liver TAG and serum TAG levels of the high-DAG group were significantly lower than those of the high-TAG group. These effects were associated with up-regulation of acyl-CoA carnitine acyltransferase (ACAT) and down-regulation of acyl-CoA DAG acyltransferase (DGAT) in the liver. However, no significant difference was observed in the activities of alanine aminotransferase and aspartate aminotransferase among the groups (P>0.05). The present results indicate that dietary DAG reduced fat accumulation in viscera and body, and these effects may be involved with up-regulation of ACAT and down-regulation of DGAT in the liver.

以菜籽油、甘油为原料，采用无溶剂体系酶法甘油解生产1，3-甘油二酯（1，3-DAG）。研究结果表明，采用硅胶吸附甘油法可明显减少间歇反应中酶活损失，显著提高酶的生产能力和使用寿命。硅胶/甘油的比例不仅影响降低酶活损失的效果，而且与甘油解反应的初速度、转化率、产物组成密切相关。硅胶/甘油为1:1（W/W）时，间歇反应酶的生产能力可提高2.3倍，酶的重复使用次数可达20次。

研究了酒精蒸馏废液全循环对玉米粉液化、糖化和发酵的影响，结果表明，当液化酶用量达到14U/g、糖化酶用量为120U/g（玉米粉）时，蒸馏废液对液化无影响，而对糖化有促进作用；蒸馏废液能一定程度地促进酵母的生长，提高酵母耗糖速率；蒸馏废液经过25次循环发酵，酒精平均浓度在15.07%，平均糖利用率91.7%。蒸馏废液全循环工艺，解决了发酵法酒精生产中的污染问题，实现了发酵法酒精清洁生产。

α-葡萄糖醛酸酶作为木聚糖降解的限速酶之一，在木聚糖类半纤维素的生物转化中起着重要的作用。海栖热袍菌Thermotoga maritima是一个嗜极端高温的厌氧细菌，其产生的极耐热性酶类具有非常可观的工业应用前景。但热袍菌属Thermotoga的基因在大肠杆菌中的表达一般较困难。研究了T-maritima中的极耐热性α-葡萄糖醛酸酶基因在大肠杆菌不同菌株中的表达水平及纯化技术。结果表明，稀有密码子AGA、AGG和AUA限制了该基因在大肠杆菌中的表达，在大肠杆菌BL21-CodonPlus（DE3）-RIL可得到高效表达，重组蛋白表达量达20%，比酶活比野生菌株提高5倍；重组蛋白经热处理和金属Ni2+的亲和层析提纯后，达到了电泳纯，提纯倍数为5.1倍，收率为55.1%。对重组菌诱导表达条件的研究表明，营养丰富的TB培养基有助于重组菌的生长，重组菌生长至OD600为0.7～0.8时添加IPTG诱导5h后重组蛋白的表达量最高。