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郑从义, Liu Yuwena, Zhang Henga, Wang Cunxin a, Li Jiea, Li Huitin a, Li Zhaoyang b, Wu Jibin b, Zheng Congyi b, ∗
Thermochimica Acta 407(2003)113-116,-0001,():
-1年11月30日
In this study, microcalorimetric measurements were carried out on PK-15 cell line persistently infected by cholera swine fever virus (CSFV) and BHK-21 cell line infected by foot and mouth disease virus (FMDV). The aim of this investigation was to investigate the difference of energy metabolism between the different kinds of virus infections-persistent infection and cytocidal infection. The thermogenesis curves determined by using a LKB 2277 Bioactivity Monitor were significantly different. From these thermogenic curves, the maximum heat production rate Pm and total heat output Q are obtained. The results show that energy metabolism is different between persistent infection and cytocidal infection.
Virus, Microcalorimetry, Metabolism, Persistent infection, Cytocidal infection
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【期刊论文】丙肝病毒全基因组cDNA克隆侵染细胞培养体系的建立
郑从义, 姚相杰①, 郭佳①, 郑从义①*, 方呈祥①, 屈三甫①, 陈震球②, 李中红②, 李信墙②*, 李卫云②
科学通报,2004,49(10):965~970,-0001,():
-1年11月30日
以含有丙肝病毒全基因组 cDNA的重组质粒(pHCV)为材料, 通过基因转染、重组痘病毒辅助使之在HeLa细胞中表达HCV病毒体,建立了一种新的HCV体外细胞培养系统。采用RT-PCR,荧光定量RT-PCR,链特异性RT-PCR,免疫印迹,免疫电子显微镜和电子显微镜负染技术跟踪检测HCV的增殖效率,结果显示,该培养体系中有HCV正链RNA的合成和HCV结构蛋白、非结构蛋白的表达,并组装成直径约47nm的HCV病毒体,病毒体可被偶联有胶体金的抗HCV结构蛋白E2的单抗标记;该体系产生的HCV病毒体滴度达107基因拷贝/mL,远远高于病人血清中的HCV基因拷贝数,也高于迄今报道的任何一种HCV细胞培养体系的病毒滴度,检测结果证明,转染细胞裂解上清中的HCV病毒体可以感染肝癌细胞系Huh7,并在感染细胞中增殖和合成负链RNA中间体,病毒滴度达106基因拷贝/mL。
丙型肝炎病毒(, HCV), , 全基因组, 侵染性cDNA, 克隆, 重组痘病毒, 细胞培养体系
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郑从义, Feng Liang, a Ping Wang, a Xiang Zhou, a Tao Li, b Zhaoyang Li, c Huakuan Lin, d, Dongzhao Gao, d Congyi Zhengc and Chengtai Wua, *
Bioorganic & Medicinal Chemistry Letters 14(2004)1901-1904,-0001,():
-1年11月30日
The stability constants for the formation of nickle(II) and cobalt(II) complexes of the ligand [1,4,7]triazecan-9-ol (L) were presented. Antitumor activity of two complexes was reported. Nuclei of [NiL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to cell cycle, and optimal induction of apoptosis was found by Flow-Cytometric analysis. But CoL complex did not exhibit introduction effects to BEL-7402 cells apoptosis; and could not perturb cell cycle. NiL and CuL complexes could cleave supercoiled DNA (pBR 322 DNA) to nicked and linear DNA, and DNA of cells treated with NiL or CuL complex was obviously damaged; while CoL complex only could cleave supercoiled DNA (pBR 322 DNA) to nicked DNA, and DNA of cells treated with CoL complex had no significant difference with control.
Macrocyclic polyamines, Metal complexes, Antitumor activity, DNA cleavage.,
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郑从义, XINCHEN ZHANG, HUSHENG ZHANG*, CONGYI ZHENG, CHAOYANG LI, XINSONG ZHANG and WEI XIONG
Cell Biology International 2002, Vol. 26, No.7, 599-603,-0001,():
-1年11月30日
Extremely low frequency (ELF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100T • M1, pulse width of 20-200 ms and frequency of 0.16-1.34Hz treatment of mice can inhibit murine malignant tumour growth, as seen from analyses at different hierarchical levels, from organism, organ, to tissue, and down to cell and macromolecules. Such magnetic fields induce apoptosis of cancer cells, and arrest neoangiogenesis, preventing a supply developing to the tumour. The growth of sarcomas might be amenable to such new method of treatment.
extremely low frequency (, ELF), pulsed-gradient magnetic field, malignant tumour, inhibitory, biological levels.,
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【期刊论文】Copper Complex of Hydroxyl-Substituted Triazamacrocyclic Ligand and Its Antitumor Activity
郑从义, Feng Liang, a Chengtai Wu, a, * Huakuan Lin, b Tao Li, c Dongzhao Gao, b Zhaoyang Li, d Jun Wei, d Congyi Zhengd and Mengxiang Sund
Bioorganic & Medicinal Chemistry Letters 13(2003)2469-2472,-0001,():
-1年11月30日
The protonation constants and the stability constants for the formation of copper (II) complex of the ligand [1,4,7] Triazecan-9-ol (L) were presented. Antitumor activity of CuL complex was reported. Preliminary pharmacological tests showed that it had antitumor activity against HXO-RB44 and BEL-7402 cell lines in vitro. Nuclei of [CuL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to BEL-7402 cell lines cycle and further studies showed that it could cleave supercoiled DNA (pBR 322) to nicked and linear DNA.
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